Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy

ARIBAS, Olgun Kadir; Esme, Hidir; Arslan Kasdoagan, Zumrut Ela; CALIK, Mustafa; Calik, Saniye Goknil; AVUNDUK, MUSTAFA CIHAT

doi:10.3906/sag-1802-187

Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy

Saniye ÇALIK, (KTO Karatay Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Acil ve İlk Yardım Anabilim Dalı, Konya, Türkiye)

Mustafa ÇELİK, (Konya Eğitim ve Araştırma Hastanesi, Sağlık Bilimleri Üniversitesi, Göğüs Cerrahisi Anabilim Dalı, Konya, Türkiye)

Zümrüt Ela ARSLAN KASDOĞAN, (Sağlık Bilimleri Üniversitesi, Konya Eğitim ve Araştırma Hastanesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Konya, Türkiye)

Mustafa Cihat AVUNDUK, (Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Patoloji Anabilim Dalı, Konya, Türkiye)

Olgun Kadir ARIBAŞ, (Gazi Üniversitesi, Tıp Fakültesi, Göğüs Cerrahisi Anabilim Dalı, Ankara, Türkiye)

Hidir ESME (KTO Karatay Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Acil ve İlk Yardım Anabilim Dalı, Konya, Türkiye)

Turkish Journal of Medical Sciences

4 0

Yıl: 2021 Cilt: 51 Sayı: 2 Sayfa Aralığı: 857 - 863 Metin Dili: İngilizce DOI: 10.3906/sag-1802-187 İndeks Tarihi: 25-01-2022

Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy

Öz:

Background/aim: We aimed to investigate the topical application of mitomycin-C (MMC) after the conventional tracheostomy in a rabbit model. Materials and methods: Twenty-four male New Zealand White rabbits were randomly divided among 3 equal groups (n: 8). Tracheostomies were performed on 16 subjects. Group 1 which served as a control for all tracheal measurements. After tracheostomy, we applied sterile saline (group 2) or MMC at 0.8 mg/mL (group 3) around the tracheotomy site for 5 min. At the 3rd week after surgery, all tracheas were subjected to morphometric and histopathological examinations, including tracheal lumen diameter (LD), number of capillary vessels (CV), subepithelial tissue thickness (SETT), fibroblasts, and inflammatory cells (IC). Results: There was a statistically significant difference between the two tracheostomy groups themselves and the control group for LD (p= 0.035), CV (p = 0.006), SETT, fibroblasts, and IC (p < 0.001). Histopathological analysis showed the decreased LD, CV, SETT, IC, and fibroblasts compared to MMC with tracheostomy groups. MMC was more effective than saline for LD, CV, SETT, IC, and fibroblasts. Conclusion: Wound healing modulation may prevent scar formation. Fibrosis decreased following tracheostomy in the group treated with MMC. Fibroblasts appear to be key cells mediating these effects.Key words: Tracheostomy, tracheal epithelia, rabbits, mitomycin-C (MMC)

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Cardone G, Lepe M. Tracheostomy: complications in fresh postoperative and late postoperative settings. Clinical Pediatric Emergency Medicine 2010; 11 (2): 122-130. doi: 10.1016/j. cpem.2010.04.001
2. Lissauer M.E. Benefit, timing, and technique of tracheostomy. Current Problems in Surgery 2013; 50 (10): 494-499. doi: 10.1067/j.cpsurg.2013.08.015
3. Tobler WD, Agarwal S. Tracheostomy current surgical therapy: expert consult. Cameron JL, Cameron AM (editors). 11th ed. 1241. Philadelphia, PA, USA: Elsevier Saunders 2014. p.1238.
4. Coyle MJ, Shrimpton A, Perkins C, Fasanmade A, Godden D. First do no harm: should routine tracheostomy after oral and maxillofacial oncological operations be abandoned? British Journal of Oral and Maxillofacial Surgery 2012; 50 (8): 732-735. doi: 10.1016/j.bjoms.2012.01.003
5. Arbağ H, Avunduk MC, Özer B, Öztürk K, Ülkü CH. Increased expression of epidermal growth factor receptors in the tracheal epithelia after topical mitomycin-C in rabbits. Auris Nasus Larynx 2005; 32: 65-70. doi: 10.1016/j. anl.2004.11.011
6. Veen EJD, Dikkers FG. Topical use of MMC in the upper aerodigestive tract: a review on the side effects. European Archives of Oto-Rhino-Laryngology 2010; 267 (3): 327-334. doi: 10.1007/s00405-009-1151-0
7. Iñiguez-Cuadra R, San Martín Prieto J, Iñiguez-Cuadra M, Zúñiga Erranz S, Jofré Pavez D et al. Effect of mitomycin in the surgical treatment of tracheal stenosis. Archives of Otolaryngology – Head & Neck Surgery 2008; 134 (7): 709-714. doi: 10.1001/archotol.134.7.709
8. Leary S, Underwood W, Anthony R, Cartner S, Corey D et. al. AVMA Guidelines for the Euthanasia of Animals: 2013 ed. Schaumburg, IL, USA: American Veterinary Medical Association; 2013. p. 1102
9. Lewis S, Earley M, Rosenfeld R, Silverman J. Systematic review for surgical treatment of adult and adolescent laryngotracheal stenosis. Laryngoscope 2017; 127 (1): 191-198. doi: 10.1002/ lary.26151
10. Şen S, Meteoğlu I, Ogurlu M, Şen S, Derincegöz OO et al. Topical heparin: a promising agent for the prevention of tracheal stenosis in airway surgery. Journal of Surgical Research 2009; 157 (1): 23-29. doi: 10.1016/j.jss.2009.01.028
11. Herrak L, Ahid S, Abouqal R, Lescot B, Gharbi N. Tracheal stenosis after intubation and/or tracheostomy. Egyptian Journal of Chest Diseases and Tuberculosis 2014; 63 (1): 233-237. doi: 10.1016/j.ejcdt.2013.10.015
12. Cincik H, Güngör A, Çakmak A, Ömeroğlu A, Poyrazoğlu E et al. The effects of mitomycin C and 5-fluorouracil/triamcinolone on fibrosis/scar tissue formation secondary to subglottic trauma (experimental study). American Journal of Otolaryngology 2005; 26: 45-50. doi: 10.1016/j.amjoto.2003.07.002
13. Tsakiridis K, Darwiche K, Visouli AN, Zarogoulidis P, Machairiotis N et al. Management of complex benign post-tracheostomy tracheal stenosis with bronchoscopic insertion of silicon tracheal stents, in patients with failed or contraindicated surgical reconstruction of trachea. Journal of Thoracic Disease 2012; 4 (1): 32-40. doi: 10.3978/j.issn.2072-1439. 2012.s002
14. Fatimi SH, Qasim Raza M, Ghani A, Shah N, Ashfaq A. Tracheal reconstruction for complex acute tracheal stenosis annals of medicine and surgery. Annals of Medicine and Surgery (London) 2013; 4-2 (2): 57-59. doi: 10.1016/S2049- 0801(13)70038-4
15. Grillo HC. Stents and sense. Annals of Thoracic Surgery 2000; 70: 1142. doi: 10.1016/s0003-4975(00)01796-3
16. Marel M, Pekarek Z, Spasova I, Pafko P, Schutzner J et al. Management of benign stenosis of the large airways in the university hospital in Prague, Czech Republic, in 1998-2003. Respiration 2005; 72 (6): 622-628. doi: 10.1159/000089578

APA	Calik S, CALIK M, Arslan Kasdoagan Z, AVUNDUK M, ARIBAS O, Esme H (2021). Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy. , 857 - 863. 10.3906/sag-1802-187
Chicago	Calik Saniye Goknil,CALIK Mustafa,Arslan Kasdoagan Zumrut Ela,AVUNDUK MUSTAFA CIHAT,ARIBAS Olgun Kadir,Esme Hidir Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy. (2021): 857 - 863. 10.3906/sag-1802-187
MLA	Calik Saniye Goknil,CALIK Mustafa,Arslan Kasdoagan Zumrut Ela,AVUNDUK MUSTAFA CIHAT,ARIBAS Olgun Kadir,Esme Hidir Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy. , 2021, ss.857 - 863. 10.3906/sag-1802-187
AMA	Calik S,CALIK M,Arslan Kasdoagan Z,AVUNDUK M,ARIBAS O,Esme H Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy. . 2021; 857 - 863. 10.3906/sag-1802-187
Vancouver	Calik S,CALIK M,Arslan Kasdoagan Z,AVUNDUK M,ARIBAS O,Esme H Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy. . 2021; 857 - 863. 10.3906/sag-1802-187
IEEE	Calik S,CALIK M,Arslan Kasdoagan Z,AVUNDUK M,ARIBAS O,Esme H "Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy." , ss.857 - 863, 2021. 10.3906/sag-1802-187
ISNAD	Calik, Saniye Goknil vd. "Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy". (2021), 857-863. https://doi.org/10.3906/sag-1802-187

APA	Calik S, CALIK M, Arslan Kasdoagan Z, AVUNDUK M, ARIBAS O, Esme H (2021). Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy. Turkish Journal of Medical Sciences, 51(2), 857 - 863. 10.3906/sag-1802-187
Chicago	Calik Saniye Goknil,CALIK Mustafa,Arslan Kasdoagan Zumrut Ela,AVUNDUK MUSTAFA CIHAT,ARIBAS Olgun Kadir,Esme Hidir Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy. Turkish Journal of Medical Sciences 51, no.2 (2021): 857 - 863. 10.3906/sag-1802-187
MLA	Calik Saniye Goknil,CALIK Mustafa,Arslan Kasdoagan Zumrut Ela,AVUNDUK MUSTAFA CIHAT,ARIBAS Olgun Kadir,Esme Hidir Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy. Turkish Journal of Medical Sciences, vol.51, no.2, 2021, ss.857 - 863. 10.3906/sag-1802-187
AMA	Calik S,CALIK M,Arslan Kasdoagan Z,AVUNDUK M,ARIBAS O,Esme H Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy. Turkish Journal of Medical Sciences. 2021; 51(2): 857 - 863. 10.3906/sag-1802-187
Vancouver	Calik S,CALIK M,Arslan Kasdoagan Z,AVUNDUK M,ARIBAS O,Esme H Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy. Turkish Journal of Medical Sciences. 2021; 51(2): 857 - 863. 10.3906/sag-1802-187
IEEE	Calik S,CALIK M,Arslan Kasdoagan Z,AVUNDUK M,ARIBAS O,Esme H "Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy." Turkish Journal of Medical Sciences, 51, ss.857 - 863, 2021. 10.3906/sag-1802-187
ISNAD	Calik, Saniye Goknil vd. "Effects of topical mitomycin-C on the tracheal epithelia of rabbits following tracheostomy". Turkish Journal of Medical Sciences 51/2 (2021), 857-863. https://doi.org/10.3906/sag-1802-187