Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway

Sahin, Taha Koray; Guner, Gurkan; Aktepe, Oktay Halit; yalcin, suayib; arık, zafer

doi:10.3906/sag-2005-413

Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway

Oktay Halit AKTEPE, (Hacettepe Üniversitesi, Tıp Fakültesi, Tıbbi Onkoloji Anabilim Dalı, Ankara, Türkiye)

Taha Koray ŞAHİN, (Hacettepe Üniversitesi, Tıp Fakültesi İç Hastalıkları Anabilim Dalı, Ankara, Türkiye)

Gürkan GÜNER, (Hacettepe Üniversitesi, Tıp Fakültesi, Tıbbi Onkoloji Anabilim Dalı, Ankara, Türkiye)

Zafer ARIK, (Hacettepe Üniversitesi, Tıp Fakültesi, Tıbbi Onkoloji Anabilim Dalı, Ankara, Türkiye)

Şuayip YALÇIN (Hacettepe Üniversitesi, Tıp Fakültesi, Tıbbi Onkoloji Anabilim Dalı, Ankara, Türkiye)

Turkish Journal of Medical Sciences

11 0

Yıl: 2021 Cilt: 51 Sayı: 1 Sayfa Aralığı: 368 - 374 Metin Dili: İngilizce DOI: 10.3906/sag-2005-413 İndeks Tarihi: 26-01-2022

Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway

Öz:

Background/aim: Lycopene is associated with anticancer effects in various tumor types. However, the exact underlying mechanisms of action of lycopene in human cervical cancer remain to be determined. This study aimed to determine anticancer efficacy and mechanism of lycopene in human cervical carcinoma (HeLa) cells. Materials and methods: HeLa cells were treated with cisplatin (1 µM) alone, lycopene (10 µM) alone, and in combination for 72 h. The cell viability of HeLa cells was assessed via MTS assay. Western blot was used to analyze the expression levels of the nuclear factor-kappa B (NF-κB), B-cell-associated X protein (Bax), nuclear factor erythroid 2-related factor (Nrf2), and B-cell lymphoma 2 (Bcl-2). Results: We found that lycopene acts as a synergistic agent with cisplatin in preventing the growth of HeLa cells. The rates of HeLa cells’ viability were 65.6% and 71.1% with lycopene and cisplatin treatment alone compared to the control group, respectively (P < 0.001). The inhibitory effect of cisplatin was enhanced with lycopene addition by declining the cell viability to 37.4% (P < 0.0001). Lycopene treatment significantly increased Bax expression (P < 0.0001) and decreased Bcl-2 expression (P < 0.0001) in HeLa cells. Furthermore, lycopene markedly activated the Nrf2 expression (P < 0.001) and suppressed the NF-κB signaling pathway (P < 0.0001). Conclusion: Lycopene increases the sensitization of cervical cancer cells to cisplatin via inhibition of cell viability, up-regulation of Bax expression, and down-regulation of Bcl-2 expression. Furthermore, the anticancer effect of lycopene might be also associated with suppression of NF-κB-mediated inflammatory responses, and modulation of Nrf2-mediated oxidative stress. The results of the present study suggest that lycopene and concurrent cisplatin chemotherapy might have a role in improving the treatment of cervical cancer.Key words: Lycopene, cervix cancer, cancer treatment, chemotherapy

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APA	Aktepe O, Sahin T, Guner G, arık z, yalcin s (2021). Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway. , 368 - 374. 10.3906/sag-2005-413
Chicago	Aktepe Oktay Halit,Sahin Taha Koray,Guner Gurkan,arık zafer,yalcin suayib Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway. (2021): 368 - 374. 10.3906/sag-2005-413
MLA	Aktepe Oktay Halit,Sahin Taha Koray,Guner Gurkan,arık zafer,yalcin suayib Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway. , 2021, ss.368 - 374. 10.3906/sag-2005-413
AMA	Aktepe O,Sahin T,Guner G,arık z,yalcin s Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway. . 2021; 368 - 374. 10.3906/sag-2005-413
Vancouver	Aktepe O,Sahin T,Guner G,arık z,yalcin s Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway. . 2021; 368 - 374. 10.3906/sag-2005-413
IEEE	Aktepe O,Sahin T,Guner G,arık z,yalcin s "Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway." , ss.368 - 374, 2021. 10.3906/sag-2005-413
ISNAD	Aktepe, Oktay Halit vd. "Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway". (2021), 368-374. https://doi.org/10.3906/sag-2005-413

APA	Aktepe O, Sahin T, Guner G, arık z, yalcin s (2021). Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway. Turkish Journal of Medical Sciences, 51(1), 368 - 374. 10.3906/sag-2005-413
Chicago	Aktepe Oktay Halit,Sahin Taha Koray,Guner Gurkan,arık zafer,yalcin suayib Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway. Turkish Journal of Medical Sciences 51, no.1 (2021): 368 - 374. 10.3906/sag-2005-413
MLA	Aktepe Oktay Halit,Sahin Taha Koray,Guner Gurkan,arık zafer,yalcin suayib Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway. Turkish Journal of Medical Sciences, vol.51, no.1, 2021, ss.368 - 374. 10.3906/sag-2005-413
AMA	Aktepe O,Sahin T,Guner G,arık z,yalcin s Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway. Turkish Journal of Medical Sciences. 2021; 51(1): 368 - 374. 10.3906/sag-2005-413
Vancouver	Aktepe O,Sahin T,Guner G,arık z,yalcin s Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway. Turkish Journal of Medical Sciences. 2021; 51(1): 368 - 374. 10.3906/sag-2005-413
IEEE	Aktepe O,Sahin T,Guner G,arık z,yalcin s "Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway." Turkish Journal of Medical Sciences, 51, ss.368 - 374, 2021. 10.3906/sag-2005-413
ISNAD	Aktepe, Oktay Halit vd. "Lycopene sensitizes the cervical cancer cells to cisplatin via targeting nuclear factorkappa B (NF-κB) pathway". Turkish Journal of Medical Sciences 51/1 (2021), 368-374. https://doi.org/10.3906/sag-2005-413