Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle
Yıl: 2021 Cilt: 45 Sayı: 3 Sayfa Aralığı: 380 - 387 Metin Dili: İngilizce DOI: 10.3906/vet-2011-92 İndeks Tarihi: 31-01-2022
Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle
Öz: Abstract: MyoD1 gene is a member of the myogenic differentiation gene family, which plays a crucial role in growth and development. There is a lack of information about the MyoD1variants and their phenotypic influences in Turkish Grey Steppe cattle. Therefore the present study aimed to determine the genetic variability of MyoD1 g.782G>A polymorphism and to evaluate its effects on carcass characteristics in Turkish Grey Steppe cattle. Analyses were conducted on a total of 142 Turkish Grey Steppe bulls. The PCR-RFLP technique was used for genotyping of the g.782G>A polymorphism in exon 1 of the MyoD1 gene. The population genetic parameters including compatibility with Hardy–Weinberg equilibrium, heterozygosity, number of effective alleles, the polymorphism information content, and the fixation index were calculated. Statistical analysis was carried out using the least square methods of the general linear model procedure. Results revealed that BB was the preponderant genotype (41.55%) but the AA genotype exhibited a remarkable close frequency (39.44%). Accordingly, allelic frequencies were very close to each other (A:0.49; B:0.51). The χ2 test revealed that the corresponding MyoD1 locus did not conform to Hardy–Weinberg equilibrium (p < 0.001). Heterozygosity, number of effective alleles, and the polymorphism information content values were 0.4998, 1.9992, and 0.3749, respectively. Moreover, the fixation index value was found to be 0.6195. These results indicated that MyoD1 g.782G>A polymorphism is a mildly informative genetic marker for Turkish Grey Steppe cattle. Statistical analysis indicated that the studied MyoD1 locus was significantly associated with chilling loss, carcass bone content, and carcass length (p < 0.05). The AA genotype was characterized by a higher chilling loss percentage but lower carcass length and bone content compared to BB and heterozygous genotypes. This study may provide valuable information regarding cattle carcass assessment and improvement through marker-assisted selection.Key words: Cattle, MyoD1, carcass characteristics, SNP
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Hayes B, Goddard ME. The distribution of the effects of genes affecting quantitative traits in livestock. Genetics Selection Evolution 2001; 33: 209-229. doi: 10.1186/1297-9686-33-3-209
- 2. Ibeagha-Awemu EM, Kgwatalala P, Zhao X. A critical analysis of production-associated DNA polymorphisms in the genes of cattle, goat, sheep, and pig. Mammalian Genome 2008; 19: 591- 617. doi: 10.1007/s00335-008-9141-x
- 3. Bhuiyan M, Kim N, Cho Y, Yoon D, Kim KS et al. Identification of SNPs in MYOD gene family and their associations with carcass traits in cattle. Livestock Science 2009; 126: 292-297. doi: 10.1016/j.livsci.2009.05.019
- 4. Te Pas M, Soumillion A, Harders F, Verburg F, Van den Bosch T et al. Influences of myogenin genotypes on birth weight, growth rate, carcass weight, backfat thickness, and lean weight of pigs. Journal of Animal Science 1999; 77 (9): 2352-2356. doi: 10.2527/1999.7792352x
- 5. Ujan J, Zan L, Wang H, Ujan S, Adoligbe C et al. Lack of an association between a single nucleotide polymorphism in the bovine myogenic determination 1 (MyoD1) gene and meat quality traits in indigenous Chinese cattle breeds. Geneticsand Molecular Research 2011; 10 (3): 2213-2222. doi: 10.4238/ vol10-3gmr1141
- 6. Zhao X, Yu Q, Huang L, Liu QX. Patterns of positive selection of the myogenic regulatory factor gene family in vertebrates. PLoS One 2014; 9: e92873. doi: 10.1371/journal.pone.0092873
- 7. Du X, Gan Q, Yuan Z, Gao X, Zhang L et al. Polymorphism of MyoD1 and Myf6 genes and associations with carcass and meat quality traits in beef cattle. Geneticsand Molecular Research 2013; 12 (4): 6708-6717. doi: 10.4238/2013.December.13.4
- 8. Casas E, Shackelford S, Keele J, Koohmaraie M, Smith T et al. Detection of quantitative trait loci for growth and carcass composition in cattle. Journal of Animal Science 2003; 81 (12): 2976-2983. doi: 10.2527/2003.81122976x
- 9. Świerczek B, Ciemerych MA, Archacka K. From pluripotency to myogenesis: a multistep process in the dish. Journal of Muscle Research and Cell Motility 2015; 36: 363-375. doi: 10.1007/ s10974-015-9436-y
- 10. Zhou D, Xu H, Chen W, Wang Y, Zhang M et al. Study on the transcriptional regulatory mechanism of the MyoD1 gene in Guanling bovine. Royal Society of Chemistry Advances 2018; 8: 12409-12419. doi: 10.1039/C7RA11795G
- 11. Pariset L, Mariotti M, Nardone A, Soysal MI, Ozkan E et al. Relationships between Podolic cattle breeds assessed by single nucleotide polymorphisms (SNPs) genotyping. Journalof Animal Breeding Genetics 2010; 127 (6): 481-488. doi: 10.1111/j.1439- 0388.2010.00868.x
- 12. Soysal M, Kök S. The last survivors of Grey cattle resisting extinction. A case study of characteristics and sustainability of traditional systems of native Grey cattle breeds. Options Méditerranéenes A 2006; 78: 55-63.
- 13. Trukhachev V, Skripkin V, Telegina E, Yatsyk O, Golovanova N et al. Associations between newly discovered polymorphisms of the MyoD1 gene and body parameters in Stavropol breed rams. Bulgarian Journal of Veterinary Medicine 2018; 21 (1): 28-39. doi: 10.15547/bjvm.1069
- 14. Dong X, Cao H, Mao H, Hong Q, Yin Z. Association of MyoD1 gene polymorphisms with meat quality traits in domestic pigeons (Columba livia). The Journal of Poultry Science 2018; 56 (1): 20-26. doi: 10.2141/jpsa.0170182
- 15. Li W, Zhang Y, Wang D. The polymorphism of MYOD1 gene exon 4 and its association with body size in cherry valley duck. Genomics and Applied Biology 2015; 34 (5): 950-954.
- 16. Lee E, Kim J, Lim K, Ryu Y, Jeon W et al. Effects of variation in porcine MYOD1 gene on muscle fiber characteristics, lean meat production, and meat quality traits. Meat Science 2012; 92 (1): 36-43. doi: 10.1016/j.meatsci.2012.03.018
- 17. Li JF, Liu D, Yu H. The SNPs analysis of the exons of three genes of MyoD gene family in seven swine breeds (line). Agricultural Sciences in China 2005; 4: 949-952.
- 18. Liu M, Peng J, Xu DQ, Zheng R, Li FE et al. Association of MYF5 and MYOD1 gene polymorphisms and meat quality traits in Large White × Meishan F2 pig populations. Biochemical Genetics 2008; 46: 720-732. doi: 10.1007/s10528-008-9187-1
- 19. Verner J, Humpolíček P, Knoll A. Impact of MYOD family genes on pork traits in Large White and Landrace pigs. Journal of Animal Breeding and Genetics 2007; 124 (2): 81-85. doi: 10.1111/j.1439-0388.2007.00639.x
- 20. Tian L, Xu SZ, Yue WB, Li JY, Gao X et al. Relationship between genotypes at MyoD locus and carcass traits in cattle. Yi Chuan 2007; 29 (3): 313-318. doi: 10.1360/yc-007-0313.
- 21. Pfuhl R, Bellmann O, Kühn C, Teuscher F, Ender K et al. Beef versus dairy cattle: a comparison of feed conversion, carcass composition, and meat quality. Archives Animal Breeding 2007; 50 (1): 59-70.
- 22. Ardicli S, Dincel D, Blaci F. Determining the yields and percentages of retail cuts from holstein bull carcasses marketed in South Marmara Region of Turkey. Kocatepe Veterinary Journal 2018; 11 (3): 223-231. doi: 10.30607/kvj.390226
- 23. Green M, Sambrook J. Isolation of high-molecular-weight DNA from mammalian cells using proteinase K and phenol. In: Molecular Cloning: A Laboratory Manual. 4th ed. Cold Spring Harbor, New York, USA: Cold Spring Harbor Laboratory Press; 2012. pp. 47-48.
- 24. Kalinowski ST, Taper ML, Marshall TC. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Molecular Ecology 2007; 16 (5): 1099-1106. doi: 10.1111/j.1365- 294X.2007.03089.x
- 25. Nei M, Roychoudhury A. Sampling variances of heterozygosity and genetic distance. Genetics 1974; 76: 379-390.
- 26. Botstein D, White RL, Skolnick M, Davis RW. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics 1980; 32: 314-331.
- 27. Falconer DS, Mackay TFC. Introduction to quantitative genetics. Harlow, England: Pearson Education Ltd; 1996.
- 28. Trakovická A, Moravčíková N, Kasarda R. Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle. Acta Biochimica Polonica 2013; 60 (4): 783-787. doi: 10.18388/abp.2013_2058
- 29. Ardicli S, Samli H, Vatansever B, Soyudal B, Dincel Det al. Comprehensive assessment of candidate genes associated with fattening performance in Holstein–Friesian bulls. Archives Animal Breeding 2019; 62 (1): 9-32. doi: 10.5194/aab-62-9- 2019
- 30. Chu M, Yan P, Liang C, Guo X, Pei J et al. Association between polymorphism of MyoD1 gene and growth traits in Yak. China Animal Husbandry & Veterinary Medicine 2012; 29: 111-113.
- 31. Rexroad CE, Bennett GL, Stone RT, Keele JW, Fahrenkrug SC et al. Comparative mapping of BTA15 and HSA11 including a region containing a QTL for meat tenderness. Mammalian Genome 2001; 12: 561-565. doi: 10.1007/s003350020028
| APA | Ardicli S, OZDEN C (2021). Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle. , 380 - 387. 10.3906/vet-2011-92 |
| Chicago | Ardicli Sena,OZDEN COBANOGLU Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle. (2021): 380 - 387. 10.3906/vet-2011-92 |
| MLA | Ardicli Sena,OZDEN COBANOGLU Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle. , 2021, ss.380 - 387. 10.3906/vet-2011-92 |
| AMA | Ardicli S,OZDEN C Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle. . 2021; 380 - 387. 10.3906/vet-2011-92 |
| Vancouver | Ardicli S,OZDEN C Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle. . 2021; 380 - 387. 10.3906/vet-2011-92 |
| IEEE | Ardicli S,OZDEN C "Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle." , ss.380 - 387, 2021. 10.3906/vet-2011-92 |
| ISNAD | Ardicli, Sena - OZDEN, COBANOGLU. "Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle". (2021), 380-387. https://doi.org/10.3906/vet-2011-92 |
| APA | Ardicli S, OZDEN C (2021). Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle. Turkish Journal of Veterinary and Animal Sciences, 45(3), 380 - 387. 10.3906/vet-2011-92 |
| Chicago | Ardicli Sena,OZDEN COBANOGLU Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle. Turkish Journal of Veterinary and Animal Sciences 45, no.3 (2021): 380 - 387. 10.3906/vet-2011-92 |
| MLA | Ardicli Sena,OZDEN COBANOGLU Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle. Turkish Journal of Veterinary and Animal Sciences, vol.45, no.3, 2021, ss.380 - 387. 10.3906/vet-2011-92 |
| AMA | Ardicli S,OZDEN C Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle. Turkish Journal of Veterinary and Animal Sciences. 2021; 45(3): 380 - 387. 10.3906/vet-2011-92 |
| Vancouver | Ardicli S,OZDEN C Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle. Turkish Journal of Veterinary and Animal Sciences. 2021; 45(3): 380 - 387. 10.3906/vet-2011-92 |
| IEEE | Ardicli S,OZDEN C "Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle." Turkish Journal of Veterinary and Animal Sciences, 45, ss.380 - 387, 2021. 10.3906/vet-2011-92 |
| ISNAD | Ardicli, Sena - OZDEN, COBANOGLU. "Genetic variation in the bovine myogenic determination factor 1 (g.782G>A polymorphism) and its influence on carcass traits in Turkish Grey Steppe cattle". Turkish Journal of Veterinary and Animal Sciences 45/3 (2021), 380-387. https://doi.org/10.3906/vet-2011-92 |
