Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat
Yıl: 2021 Cilt: 45 Sayı: 3 Sayfa Aralığı: 411 - 418 Metin Dili: İngilizce DOI: 10.3906/vet-2008-90 İndeks Tarihi: 31-01-2022
Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat
Öz: Abstract: The accurate estrus detection is essential for successful reproduction. Many scientists reported the importance of pheromones for accurate detection of estrus. In addition, the choice of the appropriate device and the appropriate extraction solvent is important for obtaining quickly and more estrus-specific pheromones. In this study, sweat samples were collected in heat cows and volatile organic compounds (VOCs) in the gas chromatography mass spectrometry were evaluated using three different solvents (diethyl ether, dichloromethane, and hexane) for extraction. VOCs with different numbers and different ratios were identified in all three extraction solvents. As the solvent, 9 compounds were detected in diethyl ether, 8 compounds in dichloromethane, and 12 compounds in hexane. Volatile odor compounds common to all three extraction solvents were L-Proline, 1-[O-(1-oxohexyl)–N-[N-[N6-(1-oxohexyl)-N2- [N-(1-oxohexyl)–L-valyl]–L-lysyl]–L-valyl]–L-tyrosyl]-, methyl ester/Tetradecane; 1,2-Benzenedicarboxylic acid, bis (2-methylpropyl) ester; Phenol, 2,2’–methylenebis [6-(1,1-dimethylethyl)–4–methyl-; Palmitin,1,2–di-. According to the results of the analysis, the most volatile compounds were detected when using hexane as the extraction solvent.Key words: GC-MS, estrus, solvent, sweat, volatile compounds
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Baker LB. Sweating rate and sweat sodium concentration in athletes: a review of methodology and intra/interindividual variability. Sports Medicine 2017; 47 (1): 111-128. doi: 10.1007/ s40279-017-0691-5
- 2. Phillips M, Cataneo RN, Ditkoff BA, Fisher P, Greenberg J et al. Prediction of breast cancer using volatile biomarkers in the breath. Breast Cancer Research and Treatment 2006; 99: 19-21. doi: 10.1007/s10549-006-9176-1
- 3. Monedeiro F, dos Reis RB, Peria FM, Sares CTG, De Martinis BS. Investigation of sweat VOC profiles in assessment of cancer biomarkers using HS-GC-MS. Journal of Breath Research 2019; 99 (1): 19-2. doi: 10.1088/1752-7163/ab5b3c
- 4. Nordéus K, Båge R, Gustafsson H, Glinwood R, Söderquist L. A small expose on bovine pheromones: with special reference to modifications of the reproductive cycle. Chemical Signals in Vertebrates. 2016; 13: 33-42 doi: 10.1007/978-3-319-22026- 0_4
- 5. Gallardo E, Queiroz JA. The role of alternative specimens in toxicological analysis. Biomedical Chromatography 2008; 22 (8): 795-821. doi: 10.1002/bmc.1009
- 6. Ely MR, Ely BR, Chinevere TD, Lacher CP, Lukaski HC et al. Evaluation of the Megaduct sweat collector for mineral analysis. Physiological Measurement 2012; 33 (3): 385-394. doi: 10.1088/0967-3334/33/3/385
- 7. Ferhat MA, Tigrine-Kordjani N, Chemat S, Meklati BY, Chemat F. Rapid Extraction of volatile compounds using a new simultaneous microwave distillation: solvent extraction device. Chroma 2007; 65: 217-222. doi: 10.1365/s10337-006-0130-5
- 8. Zhao C, He X, Li C, Yang L, Fu Y et al. A microwave-assisted simultaneous distillation and extraction method for the separation of polysaccharides and essential oil from the leaves of Taxus chinensis Var. mairei. Applied Sciences 2016; 6 (2): 19. doi: 10.3390/app6020019
- 9. Wahl JH, Colburn HA. Solvent Extraction of Chemical Attribution Signature Compounds from Painted Wall Board: Final Report 2009; United States: N. p. doi: 10.2172/1008265
- 10. Cai Z, Qu Z, Lan Y, Zhao S, Ma X et al. Conventional, ultrasound-assisted, and accelerated-solvent extractions of anthocyanins from purple sweet potatoes. Food Chemistry 2016; 197: 266-272. doi: 10.1016/j.foodchem.2015.10.110
- 11. Aksu EH, Bozkurt T, Türk G. Farklı senkronizasyon uygulamalari ile senkronize edilen ineklerde üreme performansi üzerine vitamin E’nin etkisi. Foray Üniversitesi Sağlık Bilimleri Dergisi Veterinerlik Fakültesi Dergisi 2010; 24 (2): 71-76 (in Turkish).
- 12. Dransfield MBG, Nebel RL, Pearson RE, Warnick LD. Timing of insemination for dairy cows identified in estrus by a radiotelemetric estrus detection system. Journal of Dairy Science 1998; 81: 1874-1882.
- 13. Kennedy GA. Detecting hypocalcemia using bovine nose sweat. United States Patent 2011; No: 7.964.409.
- 14. Kumar RK, Archunan G, Jeyaraman R, Narasimhan S. Chemical characterization of bovine urine with special reference to estrus. Veterinary Research Communications 2000; 24: 445-454. doi: 10.1023/A:1006495404407
- 15. Sankar R, Archunan G. Identification of putative pheromones in bovine (Bos taurus) faeces in relation to estrus detection. Animal Reproduction Science 2008; 103 (1-2): 149-153. doi: 10.1016/j.anireprosci.2007.04.014
- 16. Baker LB. Physiology of sweat gland function: The roles of sweating and sweat composition in human health. Temperature 2019. doi: 10.1080/23328940.2019.1632145
- 17. Bahrami N, Yonekura L, Linforth R, Carvalho da Silva M, Hill S et al. Comparison of ambient solvent extraction methods for the analysis of fatty acids in non-starch lipids of flour and starch. Journal of the Science of Food and Agriculture 2013; 94 (3): 415-423. doi: 10.1002/jsfa.6449
- 18. Mozūraitis R, Kutra J, Borg-Karlson A-K, Būda V. Dynamics of putative sex pheromone components during heat periods in estrus-induced cows. Journal of Dairy Science 2017; 100 (9): 7686-7695. doi: 10.3168/jds.2016-12376
- 19. Sankar R, Archunan G. Gas chromatographic/mass spectrometric analysis of volatile metabolites in bovine vaginal fluid and assessment of their bioactivity. International Journal of Analytical Chemistry 2011; 1-7. doi: 10.1155/2011/256106
- 20. Ramesh Kumar K, Archunan G, Jeyaraman R, Narasimhan S. Chemical characterization of bovine urine with special reference to oestrus. Veterinary Research Communications 2000; 24 (7): 445-454. doi: 10.1023/A:1006495404407
- 21. Choi MJ, Oh CH. 2nd dimensional GC-MS analysis of sweat volatile organic compounds prepared by solid phase microextraction. Technol Health Care 2014; 22 (3): 481-488. doi: 10.3233/THC-140807
| APA | ANİTAŞ Ö, göncü s (2021). Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat. , 411 - 418. 10.3906/vet-2008-90 |
| Chicago | ANİTAŞ ÖZGÜL,göncü serap Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat. (2021): 411 - 418. 10.3906/vet-2008-90 |
| MLA | ANİTAŞ ÖZGÜL,göncü serap Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat. , 2021, ss.411 - 418. 10.3906/vet-2008-90 |
| AMA | ANİTAŞ Ö,göncü s Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat. . 2021; 411 - 418. 10.3906/vet-2008-90 |
| Vancouver | ANİTAŞ Ö,göncü s Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat. . 2021; 411 - 418. 10.3906/vet-2008-90 |
| IEEE | ANİTAŞ Ö,göncü s "Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat." , ss.411 - 418, 2021. 10.3906/vet-2008-90 |
| ISNAD | ANİTAŞ, ÖZGÜL - göncü, serap. "Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat". (2021), 411-418. https://doi.org/10.3906/vet-2008-90 |
| APA | ANİTAŞ Ö, göncü s (2021). Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat. Turkish Journal of Veterinary and Animal Sciences, 45(3), 411 - 418. 10.3906/vet-2008-90 |
| Chicago | ANİTAŞ ÖZGÜL,göncü serap Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat. Turkish Journal of Veterinary and Animal Sciences 45, no.3 (2021): 411 - 418. 10.3906/vet-2008-90 |
| MLA | ANİTAŞ ÖZGÜL,göncü serap Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat. Turkish Journal of Veterinary and Animal Sciences, vol.45, no.3, 2021, ss.411 - 418. 10.3906/vet-2008-90 |
| AMA | ANİTAŞ Ö,göncü s Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat. Turkish Journal of Veterinary and Animal Sciences. 2021; 45(3): 411 - 418. 10.3906/vet-2008-90 |
| Vancouver | ANİTAŞ Ö,göncü s Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat. Turkish Journal of Veterinary and Animal Sciences. 2021; 45(3): 411 - 418. 10.3906/vet-2008-90 |
| IEEE | ANİTAŞ Ö,göncü s "Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat." Turkish Journal of Veterinary and Animal Sciences, 45, ss.411 - 418, 2021. 10.3906/vet-2008-90 |
| ISNAD | ANİTAŞ, ÖZGÜL - göncü, serap. "Comparison of different extraction solvents used in GC-MS analysis for detecting volatile odor compounds in heat cow sweat". Turkish Journal of Veterinary and Animal Sciences 45/3 (2021), 411-418. https://doi.org/10.3906/vet-2008-90 |
