Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients

AHMAD, Shafiq; ALI, Ayaz; ALI, Arshad

doi:10.14744/ejmo.2021.25927

Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients

Arshad ALI, (Northwestern Polytechnical University Xian, School of Life Science, China)

Ayaz ALI, (Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, University Institute of Biochemistry and Biotechnology, Pakistan)

Shafiq AHMAD (Kohat University of Science & Technology, Department of Biotechnology & Genetic Engineering, Pakistan)

Eurasian Journal of Medicine and Oncology

1 2

Yıl: 2021 Cilt: 5 Sayı: 3 Sayfa Aralığı: 249 - 259 Metin Dili: İngilizce DOI: 10.14744/ejmo.2021.25927 İndeks Tarihi: 06-02-2022

Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients

Öz:

Objectives: Alteration in the Glutathione (GSH) and Glutathione S-Transferase (GST) family lead to various disordersincluding breast cancer. However, the role of GSH and GSTM1 in the onset of breast cancer is still not fully elucidated. Inthe present study we observed considerable deficiency in the levels of glutathione and genetic mutation in the GSTM1enzyme that influence susceptibility to breast cancer metastasis and invasion via EMT pathway.Methods: GSTM1 genotype was identified by multiplex polymerase chain reaction (PCR), real time (RT)-PCR and west ern blotting in breast tumor samples and adjacent normal control tissue (ANCT) samples. The endogenous glutathi one levels were determined by high performance liquid chromatography (HPLC).The tumor metastasis, invasion andepithelial-mesenchymal transition (EMT) biomarkers were determined by RT-PCR. Results: In present study genotyping analysis of GST investigated that genetic mutation in GSTM1 was detected inbreast cancer tissue samples. mRNA and protein expression of GSTM1 was significantly downregulated in tumor sam ples as compared to adjacent normal control tissue (ANCT) samples in breast cancer patients. Significant reductionof total glutathione (GSHt P<0.05) was observed among correlation with patient ages, stages and histological gradesof breast cancer patients. Additionally, downregulation of GSTM1 promotes EMT pathway that leads to enhanced theexpression of tumor proliferation, invasion and metastasis in breast cancer patients (p<0.05). Conclusion: The present findings suggest that GSTM1 genotype could be a potential biomarker for breast cancer pathogenesis.

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APA	ALI A, ALI A, AHMAD S (2021). Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients. , 249 - 259. 10.14744/ejmo.2021.25927
Chicago	ALI Arshad,ALI Ayaz,AHMAD Shafiq Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients. (2021): 249 - 259. 10.14744/ejmo.2021.25927
MLA	ALI Arshad,ALI Ayaz,AHMAD Shafiq Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients. , 2021, ss.249 - 259. 10.14744/ejmo.2021.25927
AMA	ALI A,ALI A,AHMAD S Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients. . 2021; 249 - 259. 10.14744/ejmo.2021.25927
Vancouver	ALI A,ALI A,AHMAD S Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients. . 2021; 249 - 259. 10.14744/ejmo.2021.25927
IEEE	ALI A,ALI A,AHMAD S "Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients." , ss.249 - 259, 2021. 10.14744/ejmo.2021.25927
ISNAD	ALI, Arshad vd. "Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients". (2021), 249-259. https://doi.org/10.14744/ejmo.2021.25927

APA	ALI A, ALI A, AHMAD S (2021). Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients. Eurasian Journal of Medicine and Oncology, 5(3), 249 - 259. 10.14744/ejmo.2021.25927
Chicago	ALI Arshad,ALI Ayaz,AHMAD Shafiq Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients. Eurasian Journal of Medicine and Oncology 5, no.3 (2021): 249 - 259. 10.14744/ejmo.2021.25927
MLA	ALI Arshad,ALI Ayaz,AHMAD Shafiq Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients. Eurasian Journal of Medicine and Oncology, vol.5, no.3, 2021, ss.249 - 259. 10.14744/ejmo.2021.25927
AMA	ALI A,ALI A,AHMAD S Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients. Eurasian Journal of Medicine and Oncology. 2021; 5(3): 249 - 259. 10.14744/ejmo.2021.25927
Vancouver	ALI A,ALI A,AHMAD S Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients. Eurasian Journal of Medicine and Oncology. 2021; 5(3): 249 - 259. 10.14744/ejmo.2021.25927
IEEE	ALI A,ALI A,AHMAD S "Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients." Eurasian Journal of Medicine and Oncology, 5, ss.249 - 259, 2021. 10.14744/ejmo.2021.25927
ISNAD	ALI, Arshad vd. "Alterations of Glutathione and GSTM1 Mutation Induce TumorMetastasis and Invasion Via EMT Pathway in Breast Cancer Patients". Eurasian Journal of Medicine and Oncology 5/3 (2021), 249-259. https://doi.org/10.14744/ejmo.2021.25927