Objective: Coronavirus disease-2019 (Covid-19) pandemic is thought to increase the workload of the healthcare professionals and the level of stress they are exposed to. The aim of this study is to examine the level of anxiety, depression, difficulty in emotion regulation and sleep quality in healthcare professionals during the pandemic. Method: Healthcare professionals were invited to this prospective study via online tools. The Beck Anxiety Scale, Beck Depression Scale, Emotional Regulation Difficulty Scale and SCOPA Sleep Scale were directed to all participants. The participants were divided into two groups according to their working (group 1) or not-working (group 2) in a Covid-19 area. Results: The study recruited 426 subjects with a mean age of 35.5 ± 7.9 years and 275 of them were in group 1. The rates of having anxiety (severe anxiety), depression (severe depression), daytime sleepiness, night insomnia and any sleep-associated pathology were detected as 33.1% (14.0%), 20,9% (3,5%), 33,1%, 30.5% and 48.0%, respectively. Anxiety and depression related parameters were similar between two groups. Night insomnia was more common in group 1, while other sleep-related pathologies were similar between the two groups. Rather than all other subscales of the Emotional Regulation Difficulty Scale, the impulse subscale score was higher in group 1. In a multivariate regression analysis, it was seen that working in a Covid-19 area had a significant effect only on the development of night insomnia. Conclusion: It was determined that sleep pathologies were more common in healthcare professionals who were working in a Covid-19 area, and those had more difficulty in controlling their impulsive behavior while experiencing negative emotions.