TY  - JOUR
TI  - A Comparative Study of MTT and WST-1 Assays in Cytotoxicity Analysis
AB  - Introduction: Cytotoxicity assays are frequently used in cell culture and drug development studies. Some of these cyto- toxicity assays may give incorrect results due to interactions of chemicals used in the assays. This study aimed to compare the results between the 3-(4 ,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and (2-(4-iodophenyl)-3-(4- nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) (WST-1) assays, using Black Sea propolis extract (BSPE) and caffeic acid phenethyl ester (CAPE) on HCT-116 and DLD-1 colorectal cancer cell lines. Methods: HCT-116 and DLD-1 cells were treated with different doses of CAPE and BSPE. The cell viability was analyzed by MTT and WST-1 cytotoxicity assays comparatively. Afterward, cell death was examined morphologically by acridine orange/ ethidium bromide staining (AO/EB) and quantitatively by Annexin V/7AAD apoptosis detection method. Results: MTT and WST-1 assays showed different viability results on two cell lines with the same doses of BSPE. However, there was no significant difference between the results of two assays on CAPE treatment with the same doses. AO/EB stain- ing confirmed cell death following BSPE and CAPE treatment. All results from Annexin V/7AAD assay were consistent with the results of the WST-1 assay, particularly for BSPE treatment. Discussion and Conclusion: In the determination of the cytotoxic effects of BSPE, WST-1 assay reflected more precise results than MTT assay. However, two assays showed similar results when the cytotoxic effects of CAPE were determined. Conse- quently, WST-1 assay is found to be more reliable than MTT assay in the cytotoxicity analysis of a natural product such as BSPE.
AU  - KOLAYLI, Sevgi
AU  - CELEP EYÜPOĞLU, Figen
AU  - SARI, CEREN
DO  - 10.14744/hnhj.2019.16443
PY  - 2021
JO  - Haydarpaşa Numune Medical Journal
VL  - 61
IS  - 3
SN  - 2630-5720
SP  - 281
EP  - 288
DB  - TRDizin
UR  - http://search/yayin/detay/489054
ER  -