In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells

Duydu, Yalçın; İPEK, Seda; ÜSTÜNDAG, AYLIN; YILMAZ, MURAT; AYDIN, CUMHUR; Chinheya, Rosa Mhlanga

doi:10.29228/jrp.53

In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells

Rosa Mhlanga CHINHEYA, (Ankara Üniversitesi, Sağlık Bilimleri Fakültesi, Adli Kimya ve Toksikoloji Anabilim Dalı, Ankara, Türkiye)

Murat YILMAZ, (Sağlık Bilimleri Üniversitesi, Gülhane Diş Hekimliği Fakültesi, Endodonti Anabilim Dalı, Ankara, Türkiye)

Aylin ÜSTÜNDAĞ, (Ankara Üniversitesi, Eczacılık Fakültesi, Farmasötik Toksikoloji Anabilim Dalı, Ankara, Türkiye)

Seda İPEK, (Ankara Üniversitesi, Eczacılık Fakültesi, Farmasötik Toksikoloji Anabilim Dalı, Ankara, Türkiye)

Yalçın DUYDU, (Ankara Üniversitesi, Eczacılık Fakültesi, Farmasötik Toksikoloji Anabilim Dalı, Ankara, Türkiye)

Cumhur AYDIN (Sağlık Bilimleri Üniversitesi, Gülhane Diş Hekimliği Fakültesi, Endodonti Anabilim Dalı, Ankara, Türkiye)

Journal of research in pharmacy (online)

13 0

Yıl: 2021 Cilt: 25 Sayı: 5 Sayfa Aralığı: 616 - 625 Metin Dili: İngilizce DOI: 10.29228/jrp.53 İndeks Tarihi: 11-02-2022

In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells

Öz:

This study evaluated the in vitro cytotoxic, apoptotic and genotoxic effects of MTA, TheraCal and Dermabond (2-octyl cyanoacrylate) on L929 mouse fibroblast cells. Culture medium was exposed to the materials for 24 hours, 1 week and 2 weeks. Cells were seeded in 96 well plates with 5x103 cells/100 μL media per well for MTT assay, 6 well plates with 2x105 cells /2 mL media per well for comet assay,12-well plates with 2x105 cells/well for apoptosis assay and cells were exposed to the material eluates. The cell viability, genotoxicity and apoptotic status were evaluated by MTT, comet and apoptosis assays respectively at three different times (24 hours, 1 week and 2 weeks). There was no statistically significant difference (p˃0.05) between MTA and Dermabond at 24 hours, 1 week and 2 weeks exposures. However, TheraCal significantly reduced the cell viability at 1-week exposure to 66.70% (p˂0.05). TheraCal caused DNA damage at all exposures and there was a statistically significant difference (p˂0.05). According to flow cytometry results, TheraCal at 24-hour and 1-week showed significantly higher cytotoxic effects compared to MTA and Dermabond. No significant differences were detected between MTA and Dermabond. As observed from the current study it can be concluded that MTA and Dermabond are biocompatible capping materials in vital endodontic treatments. Since TheraCal exhibited cytotoxic and genotoxic effects caution should be exercised when it is being used.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

[1] Ahn DK, Sims CD, Randolpf MA, O’Connor D, Butler PEM, Amarante MTJ. Craniofacial skeletal fixation using biodegradable plates and cyanoacrylate glue. Plast Reconstr Surg. 1997; 99: 1508-1515.
[2] Akbulut MB, Arpaci PU, Eldeniz AU. Effects of four novel root-end filling materials on the viability of periodontal liga-ment fibroblasts. Restor Dent Endod. 2018; 43(3): e24.
[3] Azevedo CL, Marques MM, Bombana AC. Cytotoxic effects of cyanoacrylates used as retrograde filling materials: an in vitro analysis. Pesqui Odontol Bras. 2003; 17(2): 113-118.
[4] Balaji DVR, Manikandan DD, Abirami DG, Nirmal J. Cyanoacrylate - Stick Instead Of Stitch. World J Adv Sci Res. 2019; 2(2), 31-38.
[5] Mizrahi B, Stefanescu CF, Yang C, Lawlor MW, Ko D, Langer R, Kohane DS. Elasticity and safety of alkoxyethyl cyanoacrylate tissue adhesives. Acta Biomater. 2011; 7(8): 3150–3157.
[6] Braz MG, Camargo EA, Salvadori DM, Marques ME, Ribeiro DA. Evaluation of genetic damage in human peripheral lymphocytes exposed to mineral trioxide aggregate and Portland cements. J Oral Rehabil. 2006; 33: 234-239.
[7] Cooper JM, Paige KT. Primary and revision cleft lip repairs using octyl-2-cyanoacrylate. J Craniofac Surg. 2006; 17(2): 340-343.
[8] da Silva GN, Braz MG, de Camargo EA, Salvadori DM, Ribeiro DA. Genotoxicity in primary human peripheral lymphocytes after exposure to regular and white mineral trioxide aggregate. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 102: e50-54.
[9] Hanning M, Hanning C. Nanomaterials in preventive dentistry. Nat Nanotechnol. 2010; 5: 565–569.
[10] Park HD, Kim S, Ahn K-D, Yong KE, Kim Y, Keun HD. In vitro degradation and cytotoxicity of alkyl 2‐cyanoacrylate polymers for application to tissue adhesives. J Appl Polym Sci. 2003; 89: 3272-3278.
[11] Hilton TJ. Keys to clinical success with pulp capping: a review of the literature. Oper Dent, 2009; 34(5): 615-625.
[12] Lakshmanan I, Batra SK. Protocol for Apoptosis Assay by Flow Cytometry Using Annexin V Staining Method. Bio Protoc. 2013; 3(6): e374.
[13] Lee YJ, Jung GB, Choi S, Lee G, Kim JH, Son HS, Bae H, Park H-K. Biocompatibility of a novel cyanoacrylate based tissue adhesive: Cytotoxicity and biochemical property evaluation. PLoS One. 2013; 8(11): e79761.
[14] Lessa FCR, Aranha AMF, Hebling J, Cost de Souza CA. Cytotoxic effects of White-MTA and MTA-Bio cements on odontoblast-like cells (MDPC-23). Braz Dent J. 2010; 21(1): 24-31.
[15] Collado-Gonzalez M, Garcia-Bernal D, Onate-Sanchez RE, Ortaloni-Setenerid PS, Alvarez-Muro T, Lozano A, Forner L, Moraleda JM, Rodriguez FJ. Cytotoxicity and bioactivity of various pulpotomy materials on stem cells from human exfoliated primary teeth. Int Endod J. 2017; 50: e19-e30.
[16] Naghavi N, Ghoddusi J, Sadeghnia HR, Asadpour E, Asgary S. Genotoxicity and cytotoxicity of mineral trioxide aggregate and calcium enriched mixture cements on L929 mouse fibroblast cells. Dent Mater J. 2014; 33(1): 64–69.
[17] Nilsen BW, Jensen E, Ortengren U, Michelsen VB. Analysis of organic components in resin-modified pulp capping materials: Critical considerations. Eur J Oral Sci. 2017; 125, 183–194.
[18] Poggio C, Matteo C, Alberto D, Riccardo B, Marco C, Marco C. In vitro cytotoxicity evaluation of different pulp capping materials. Arh Hig Rada Toksikol. 2015; 66: 181-188.
[19] Rattan SI, Hormesis in aging. Ageing Res Rev. 2008; 7: 63–78.
[20] Ribeiro DA, Sugui MM, Matsumoto MA, Duarte MA, Marques ME, Salvadori DM. Genotoxicity and cytotoxicity of mineral trioxide aggregate and regular and white Portland cements on Chinese hamster ovary (CHO) cells in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 101: 258- 261.
[21] Ribeiro DA, Matsumoto MA, Duarte MA, Marques ME, Salvadori DM. In vitro biocompatibility tests of two commercial types of mineral trioxide aggregate. Braz Oral Res. 2005; 19: 183-187.
[22] Ribeiro DA, Bazo AP, Franchi CAS, Marques MEA, Salvadori DMF. Chlorhexidine induces DNA damage in rat peripheral leukocytes and oral mucosal cells. J Periodontal Res. 2004; 39: 358-361.
[23] Thom DC, Davies JE, Santerre JP, Friedman S. The hemolytic and cytotoxic properties of a zeolite-containing root filling material in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003; 95: 101–108.
[24] Torabinejad M, Parirokh M, Dummer PMH. Mineral trioxide aggregate and other bioactive endodontic cements: an updated overview–part II: other clinical applications and complications. Int Endod J. 2018; 51(3): 284-317.
[25] Tsuchiya T, Studies on the standardization of cytotoxicity tests and new standard reference materials useful for evaluating the safety of biomaterials. J Biomater Appl. 1994; 5: 139-157.
[26] Zakerzadeh A, Esnaashari E, Dadfar S. In Vitro Comparison of Cytotoxicity and Genotoxicity of Three Vital Pulp Capping Materials. Iran Endod J. 2017; 12(4): 419–425.
[27] Zeferino EG, Bueno CE, Oyama LM, Ribeiro DA. Ex vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxide. Int Endod J. 2010; 43: 843-848.

APA	Chinheya R, YILMAZ M, ÜSTÜNDAG A, İPEK S, Duydu Y, AYDIN C (2021). In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells. , 616 - 625. 10.29228/jrp.53
Chicago	Chinheya Rosa Mhlanga,YILMAZ MURAT,ÜSTÜNDAG AYLIN,İPEK Seda,Duydu Yalçın,AYDIN CUMHUR In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells. (2021): 616 - 625. 10.29228/jrp.53
MLA	Chinheya Rosa Mhlanga,YILMAZ MURAT,ÜSTÜNDAG AYLIN,İPEK Seda,Duydu Yalçın,AYDIN CUMHUR In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells. , 2021, ss.616 - 625. 10.29228/jrp.53
AMA	Chinheya R,YILMAZ M,ÜSTÜNDAG A,İPEK S,Duydu Y,AYDIN C In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells. . 2021; 616 - 625. 10.29228/jrp.53
Vancouver	Chinheya R,YILMAZ M,ÜSTÜNDAG A,İPEK S,Duydu Y,AYDIN C In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells. . 2021; 616 - 625. 10.29228/jrp.53
IEEE	Chinheya R,YILMAZ M,ÜSTÜNDAG A,İPEK S,Duydu Y,AYDIN C "In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells." , ss.616 - 625, 2021. 10.29228/jrp.53
ISNAD	Chinheya, Rosa Mhlanga vd. "In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells". (2021), 616-625. https://doi.org/10.29228/jrp.53

APA	Chinheya R, YILMAZ M, ÜSTÜNDAG A, İPEK S, Duydu Y, AYDIN C (2021). In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells. Journal of research in pharmacy (online), 25(5), 616 - 625. 10.29228/jrp.53
Chicago	Chinheya Rosa Mhlanga,YILMAZ MURAT,ÜSTÜNDAG AYLIN,İPEK Seda,Duydu Yalçın,AYDIN CUMHUR In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells. Journal of research in pharmacy (online) 25, no.5 (2021): 616 - 625. 10.29228/jrp.53
MLA	Chinheya Rosa Mhlanga,YILMAZ MURAT,ÜSTÜNDAG AYLIN,İPEK Seda,Duydu Yalçın,AYDIN CUMHUR In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells. Journal of research in pharmacy (online), vol.25, no.5, 2021, ss.616 - 625. 10.29228/jrp.53
AMA	Chinheya R,YILMAZ M,ÜSTÜNDAG A,İPEK S,Duydu Y,AYDIN C In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells. Journal of research in pharmacy (online). 2021; 25(5): 616 - 625. 10.29228/jrp.53
Vancouver	Chinheya R,YILMAZ M,ÜSTÜNDAG A,İPEK S,Duydu Y,AYDIN C In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells. Journal of research in pharmacy (online). 2021; 25(5): 616 - 625. 10.29228/jrp.53
IEEE	Chinheya R,YILMAZ M,ÜSTÜNDAG A,İPEK S,Duydu Y,AYDIN C "In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells." Journal of research in pharmacy (online), 25, ss.616 - 625, 2021. 10.29228/jrp.53
ISNAD	Chinheya, Rosa Mhlanga vd. "In vitro investigation of the cytotoxic, apoptotic and genotoxic effects of pulp capping materials on L929 mouse fibroblast cells". Journal of research in pharmacy (online) 25/5 (2021), 616-625. https://doi.org/10.29228/jrp.53