Objective: This study was carried out to determine the anxiety levels of mothers whose babies were hospitalized in neonatal intensive care unit. Material and Methods: The study was a descriptive study and after the necessary permissions were obtained, it was carried out with 148 volunteered mothers whose babies were hospitalized in neonatal intensive care unit of a university hospital between November 2015 and May 2016. The data were collected with “Parent and Baby Information Form” and “State and Trait Anxiety Scale”. Results: It was determined that 56.1% (n=83) of the mothers’ participating in the study had a cesarean section (n=112; 75.7%), 35.8% (n=53) of them had graduated from high school and did not work (n=105; 70.9%). It was found that State anxiety point scores were 40.43±4.31 and Parmanent anxiety point score were 41.43±4.89. It was found that there was a statistically significant difference between maternal brestfeeding status mode of delivery and feeling ready to be discharged and State anxiety point scores; maternal working status, mode of delivery and parmanent anxiety scores. Conclusion: It was determined that the mothers’ State and Parmanent anxity scores were mild in the study.