The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines

HOTİ, Berna; KILIÇ, Ceyda Sibel; GÜRBÜZ, İlhan; Günbatan, Tuğba; BAKAR ATEŞ, Filiz; DUMAN, HAYRI

doi:10.1515/tjb-2020-0225

The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines

Filiz BAKAR ATEŞ, (Ankara Üniversitesi, Eczacılık Fakültesi, Biyokimya Bölümü, Ankara, Türkiye)

Berna HOTİ, (Ankara Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye)

İlhan GÜRBÜZ, (Gazi Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Ankara, Türkiye)

Tuğba GÜNBATAN, (Gazi Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Ankara, Türkiye)

Hayri DUMAN, (Gazi Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Ceyda Sibel KILIÇ (Ankara Üniversitesi, Eczacılık Fakültesi, Farmasötik Botanik Anabilim Dalı, Ankara, Türkiye)

Türk Biyokimya Dergisi

8 1

Yıl: 2021 Cilt: 46 Sayı: 3 Sayfa Aralığı: 281 - 291 Metin Dili: İngilizce DOI: 10.1515/tjb-2020-0225 İndeks Tarihi: 20-02-2022

The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines

Öz:

Background: The studies investigating the anticancer activities of natural products have accelerated to produce new solutions in the face of increasing cancer cases. Various Ferulago species are reported to exhibit antioxi dant, antiulcer and antimicrobial activities. Objective: This study aimed to evaluate the cytotoxic and apoptotic activities of ethanolic extracts of roots of five Ferulago species on various human cancer cell lines. Material and methods: HPLC analyses were performed by HP Agilent 1,100. The cytotoxicity were determined by MTT assay. The cell cycle arrest and Annexin V binding analyses were performed by Muse Cell Analyzer (Millipore). Results: All examined species except F. setifolia inhibited cell viability in PC3 and SW480 cells at 0.01 mg/mL and higher concentrations (p<0.05). Ferulago species inhibited cell cycle at different stages for treated cell lines. The ethanolic extracts of Ferulago species also increased Annexin V binding significantly, resulted in apoptosis (p<0.05%). In this context, F. syriaca showed the highest apoptotic activity in MCF-7 cells by increasing the apoptotic cell population to 23.54 ± 2.10% (p<0.0001). Conclusion: The findings of present study have shown that Ferulago species included in the study have potent anticancer effects and this work have the potential to result in further studies.

Anahtar Kelime:

Ferulago W. Koch Türlerinin Çeşitli Kanser Hücre Hatlarında Sitotoksik ve Apoptotik Etkinlikleri

Öz:

Amaç: Doğal ürünlerin antikanser aktivitelerini araştıran çalışmalar, artan kanser vakaları karşısında yeni çözümler üretmek için giderek hız kazanmıştır. Çeşitli Ferulago tür lerinin antioksidan, antiülser ve antimikrobiyal aktiviteler sergilediği bildirilmiştir. Bu çalışmada, beş Ferulago türünün köklerinin etanolik ekstraktlarının çeşitli insan kanser hücre hatları üzerindeki sitotoksik ve apoptotik aktivitelerinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: HPLC analizleri, HP Agilent 1,100 ile yapıldı. Sitotoksisite, MTT testi ile belirlendi. Hücre döngüsü tutulumu ve Annexin V bağlanma analizleri, Muse Cell Analyzer (Millipore) ile gerçekleştirildi. Bulgular: Elde edilen bulgulara göre, F. setifolia dışında incelenen tüm türler, PC3 ve SW480 hücrelerinde 0,01 mg/mL ve daha yüksek konsantrasyonlarda hücre canlılığını inhibe etmiştir (p<0,05). Ferulago türlerinin, ekstre uygulanan hücre hatları için farklı aşamalarda hücre döngüsünü inhibe ettiği gözlenmiştir. Ferulago türlerinin etanolik ekstreleri, Annexin V bağlanmasını önemli ölçüde artırmış ve apoptozu indüklediği belirlenmiştir (p<%0,05).Bu kapsamda, F. syriaca’nın, apoptotik hücre popülasyo nunu % 23.54 ± 2.10′a çıkararak MCF-7 hücrelerinde en yüksek apoptotik aktiviteyi gösterdiği belirlenmiştir (p<0,0001). Sonuç: Bu çalışmanın bulguları, çalışmaya dahil edilen Ferulago türlerinin güçlü antikanser etkilere sahip oldu ğunu ve bu çalışmanın daha ileri çalışmalara dayanak oluşturma potansiyeline sahip olduğunu göstermiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BAKAR ATEŞ F, HOTİ B, GÜRBÜZ İ, Günbatan T, DUMAN H, KILIÇ C (2021). The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines. , 281 - 291. 10.1515/tjb-2020-0225
Chicago	BAKAR ATEŞ Filiz,HOTİ Berna,GÜRBÜZ İlhan,Günbatan Tuğba,DUMAN HAYRI,KILIÇ Ceyda Sibel The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines. (2021): 281 - 291. 10.1515/tjb-2020-0225
MLA	BAKAR ATEŞ Filiz,HOTİ Berna,GÜRBÜZ İlhan,Günbatan Tuğba,DUMAN HAYRI,KILIÇ Ceyda Sibel The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines. , 2021, ss.281 - 291. 10.1515/tjb-2020-0225
AMA	BAKAR ATEŞ F,HOTİ B,GÜRBÜZ İ,Günbatan T,DUMAN H,KILIÇ C The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines. . 2021; 281 - 291. 10.1515/tjb-2020-0225
Vancouver	BAKAR ATEŞ F,HOTİ B,GÜRBÜZ İ,Günbatan T,DUMAN H,KILIÇ C The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines. . 2021; 281 - 291. 10.1515/tjb-2020-0225
IEEE	BAKAR ATEŞ F,HOTİ B,GÜRBÜZ İ,Günbatan T,DUMAN H,KILIÇ C "The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines." , ss.281 - 291, 2021. 10.1515/tjb-2020-0225
ISNAD	BAKAR ATEŞ, Filiz vd. "The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines". (2021), 281-291. https://doi.org/10.1515/tjb-2020-0225

APA	BAKAR ATEŞ F, HOTİ B, GÜRBÜZ İ, Günbatan T, DUMAN H, KILIÇ C (2021). The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines. Türk Biyokimya Dergisi, 46(3), 281 - 291. 10.1515/tjb-2020-0225
Chicago	BAKAR ATEŞ Filiz,HOTİ Berna,GÜRBÜZ İlhan,Günbatan Tuğba,DUMAN HAYRI,KILIÇ Ceyda Sibel The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines. Türk Biyokimya Dergisi 46, no.3 (2021): 281 - 291. 10.1515/tjb-2020-0225
MLA	BAKAR ATEŞ Filiz,HOTİ Berna,GÜRBÜZ İlhan,Günbatan Tuğba,DUMAN HAYRI,KILIÇ Ceyda Sibel The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines. Türk Biyokimya Dergisi, vol.46, no.3, 2021, ss.281 - 291. 10.1515/tjb-2020-0225
AMA	BAKAR ATEŞ F,HOTİ B,GÜRBÜZ İ,Günbatan T,DUMAN H,KILIÇ C The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines. Türk Biyokimya Dergisi. 2021; 46(3): 281 - 291. 10.1515/tjb-2020-0225
Vancouver	BAKAR ATEŞ F,HOTİ B,GÜRBÜZ İ,Günbatan T,DUMAN H,KILIÇ C The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines. Türk Biyokimya Dergisi. 2021; 46(3): 281 - 291. 10.1515/tjb-2020-0225
IEEE	BAKAR ATEŞ F,HOTİ B,GÜRBÜZ İ,Günbatan T,DUMAN H,KILIÇ C "The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines." Türk Biyokimya Dergisi, 46, ss.281 - 291, 2021. 10.1515/tjb-2020-0225
ISNAD	BAKAR ATEŞ, Filiz vd. "The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines". Türk Biyokimya Dergisi 46/3 (2021), 281-291. https://doi.org/10.1515/tjb-2020-0225