TY  - JOUR
TI  - The cytotoxic and apoptotic effects of Ferulago W. Koch extracts on various cancer cell lines
AB  - Background: The studies investigating the anticancer activities of natural products have accelerated to produce new solutions in the face of increasing cancer cases. Various Ferulago species are reported to exhibit antioxi dant, antiulcer and antimicrobial activities. Objective: This study aimed to evaluate the cytotoxic and apoptotic activities of ethanolic extracts of roots of five Ferulago species on various human cancer cell lines. Material and methods: HPLC analyses were performed by HP Agilent 1,100. The cytotoxicity were determined by MTT assay. The cell cycle arrest and Annexin V binding analyses were performed by Muse Cell Analyzer (Millipore). Results: All examined species except F. setifolia inhibited cell viability in PC3 and SW480 cells at 0.01 mg/mL and higher concentrations (p<0.05). Ferulago species inhibited cell cycle at different stages for treated cell lines. The ethanolic extracts of Ferulago species also increased Annexin V binding significantly, resulted in apoptosis (p<0.05%). In this context, F. syriaca showed the highest apoptotic activity in MCF-7 cells by increasing the apoptotic cell population to 23.54 ± 2.10% (p<0.0001). Conclusion: The findings of present study have shown that Ferulago species included in the study have potent anticancer effects and this work have the potential to result in further studies.
AU  - HOTİ, Berna
AU  - KILIÇ, Ceyda Sibel
AU  - GÜRBÜZ, İlhan
AU  - Günbatan, Tuğba
AU  - BAKAR ATEŞ, Filiz
AU  - DUMAN, HAYRI
DO  - 10.1515/tjb-2020-0225
PY  - 2021
JO  - Türk Biyokimya Dergisi
VL  - 46
IS  - 3
SN  - 1303-829X
SP  - 281
EP  - 291
DB  - TRDizin
UR  - http://search/yayin/detay/492541
ER  -