Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins

Başkol, Mevlüt; KILIÇ, Eser; HELVACI, Nazlı; GÜÇLÜ, Kenan; baskol, gulden; Özel, Merve

doi:10.1515/tjb-2019-0397

Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins

Merve ÖZEL, (Erciyes Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Kayseri, Türkiye)

Kenan GÜÇLÜ, (Kırşehir Ahievran Üniversitesi Eğitim ve Araştırma Hastanesi, Biyokimya Bölümü, Kırşehir, Türkiye)

Nazlı HELVACI, (Erciyes Üniversitesi, Tıp Fakültesi, Biyokimya Bölümü, Kayseri, Türkiye)

Eser KILIÇ, (Erciyes Üniversitesi, Tıp Fakültesi, Biyokimya Bölümü, Kayseri, Türkiye)

Mevlüt BAŞKOL, (Erciyes Üniversitesi, Tıp Fakültesi, Gastroenteroloji Anabilim Dalı, Kayseri, Türkiye)

Gülden BAŞKOL (Erciyes Üniversitesi, Tıp Fakültesi, Biyokimya Bölümü, Kayseri, Türkiye)

Türk Biyokimya Dergisi

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Yıl: 2021 Cilt: 46 Sayı: 3 Sayfa Aralığı: 299 - 305 Metin Dili: İngilizce DOI: 10.1515/tjb-2019-0397 İndeks Tarihi: 20-02-2022

Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins

Öz:

Background: Hepatic fibrosis is a complex and dynamic process similar to “wound healing” that results in the pro gressive accumulation of connective tissue. We aimed to investigate the epigenetic control of liver fibrosis and Hip po pathway in human hepatic stellate cell (HSC) line. We examined the effect of Suberoylanilide hydroxamic acid (SAHA), a histone deacetylase inhibitor on the LX2 cell line. Material and methods: 2.5 μM SAHA was treated to LX2 cell line for 2 days. Cell proliferation and apoptosis measurement were performed by Muse Cell Analyzer. Yes-Associated Protein/Transcrıptional Coactivator With Pdz-Binding Motif (YAP/TAZ) and alpha-smooth muscle actin (α-SMA) protein expression levels were measured by western blotting. Results: In our study, we observed that the SAHA treatment reduced cell viability and induced apoptosis of LX2 cells statistically. We found that SAHA treatment decreased α-SMA, YAP and TAZ proteins levels statistically. Conclusion: Decreased cell viability could be due to physiological, autophagical and also related to the apop totical mechanisms. We thought that SAHA plays an important role in the creation of the fates of the LX2 cell line.

Anahtar Kelime:

Suberoylanilid hidroksamik asit, PDZ bağlayıcı motif proteinleri ile yes ilişkili protein/ transkripsiyonel koaktivatör azaltarak LX2 hücrelerinin proliferasyonunu inhibe eder

Öz:

Amaç: Hepatik fibrozis, bağ dokusunun birikimi ile sonuçlanan “yara iyileşmesine” benzer karmaşık ve dina mik bir süreçtir. Bu çalışmada, insan hepatik stellat hücre (HSC) hattında karaciğer fibrozisinin ve Hippo yolağının epigenetik kontrolünü araştırmayı amaçladık. Histon dea setilaz inhibitörü olan Suberoylanilid hidroksamik asit (SAHA)’nın LX2 hücre hattı üzerindeki etkisini inceledik. Materyal ve metod: LX2 hücrelerine 2 gün süreyle 2.5 μM SAHA uygulandı. Hücre proliferasyonu ve apoptoz ölçüm leri, Muse Cell Analyzer cihazı ile gerçekleştirildi. Yes-asso ciated protein/ transcriptional coactivator with PDZ-binding motif protein (YAP/TAZ) ve α-düz kas aktin (α-SMA) protein ekspresyon seviyeleri, western blot analizi ile belirlendi. Bulgular: Çalışmamızda SAHA’nın, hücre canlılığını azalttığını ve LX2 hücrelerinin apopitozunu istatistiksel olarak indüklediğini gözlemledik. SAHA ‘nın α-SMA, YAP ve TAZ protein düzeylerini istatistiksel olarak düşürdüğünü bulduk. Sonuç: Hücre canlılığının azalması; fizyolojik, otofajik ve aynı zamanda apoptotik mekanizmalardan kaynaklanabilir. SAHA’nın LX2 hücrelerinin kaderinin belirlenmesinde önemli bir rol oynadığını düşünmekteyiz.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Özel M, GÜÇLÜ K, HELVACI N, KILIÇ E, Başkol M, baskol g (2021). Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins. , 299 - 305. 10.1515/tjb-2019-0397
Chicago	Özel Merve,GÜÇLÜ Kenan,HELVACI Nazlı,KILIÇ Eser,Başkol Mevlüt,baskol gulden Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins. (2021): 299 - 305. 10.1515/tjb-2019-0397
MLA	Özel Merve,GÜÇLÜ Kenan,HELVACI Nazlı,KILIÇ Eser,Başkol Mevlüt,baskol gulden Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins. , 2021, ss.299 - 305. 10.1515/tjb-2019-0397
AMA	Özel M,GÜÇLÜ K,HELVACI N,KILIÇ E,Başkol M,baskol g Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins. . 2021; 299 - 305. 10.1515/tjb-2019-0397
Vancouver	Özel M,GÜÇLÜ K,HELVACI N,KILIÇ E,Başkol M,baskol g Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins. . 2021; 299 - 305. 10.1515/tjb-2019-0397
IEEE	Özel M,GÜÇLÜ K,HELVACI N,KILIÇ E,Başkol M,baskol g "Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins." , ss.299 - 305, 2021. 10.1515/tjb-2019-0397
ISNAD	Özel, Merve vd. "Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins". (2021), 299-305. https://doi.org/10.1515/tjb-2019-0397

APA	Özel M, GÜÇLÜ K, HELVACI N, KILIÇ E, Başkol M, baskol g (2021). Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins. Türk Biyokimya Dergisi, 46(3), 299 - 305. 10.1515/tjb-2019-0397
Chicago	Özel Merve,GÜÇLÜ Kenan,HELVACI Nazlı,KILIÇ Eser,Başkol Mevlüt,baskol gulden Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins. Türk Biyokimya Dergisi 46, no.3 (2021): 299 - 305. 10.1515/tjb-2019-0397
MLA	Özel Merve,GÜÇLÜ Kenan,HELVACI Nazlı,KILIÇ Eser,Başkol Mevlüt,baskol gulden Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins. Türk Biyokimya Dergisi, vol.46, no.3, 2021, ss.299 - 305. 10.1515/tjb-2019-0397
AMA	Özel M,GÜÇLÜ K,HELVACI N,KILIÇ E,Başkol M,baskol g Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins. Türk Biyokimya Dergisi. 2021; 46(3): 299 - 305. 10.1515/tjb-2019-0397
Vancouver	Özel M,GÜÇLÜ K,HELVACI N,KILIÇ E,Başkol M,baskol g Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins. Türk Biyokimya Dergisi. 2021; 46(3): 299 - 305. 10.1515/tjb-2019-0397
IEEE	Özel M,GÜÇLÜ K,HELVACI N,KILIÇ E,Başkol M,baskol g "Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins." Türk Biyokimya Dergisi, 46, ss.299 - 305, 2021. 10.1515/tjb-2019-0397
ISNAD	Özel, Merve vd. "Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins". Türk Biyokimya Dergisi 46/3 (2021), 299-305. https://doi.org/10.1515/tjb-2019-0397