TY  - JOUR
TI  - Suberoylanilide hydroxamic acid inhibits LX2 cells proliferation via decreasing yes-associated protein/transcriptional coactivator with PDZ-binding motif proteins
AB  - Background: Hepatic fibrosis is a complex and dynamic process similar to “wound healing” that results in the pro gressive accumulation of connective tissue. We aimed to investigate the epigenetic control of liver fibrosis and Hip po pathway in human hepatic stellate cell (HSC) line. We examined the effect of Suberoylanilide hydroxamic acid (SAHA), a histone deacetylase inhibitor on the LX2 cell line. Material and methods: 2.5 μM SAHA was treated to LX2 cell line for 2 days. Cell proliferation and apoptosis measurement were performed by Muse Cell Analyzer. Yes-Associated Protein/Transcrıptional Coactivator With Pdz-Binding Motif (YAP/TAZ) and alpha-smooth muscle actin (α-SMA) protein expression levels were measured by western blotting. Results: In our study, we observed that the SAHA treatment reduced cell viability and induced apoptosis of LX2 cells statistically. We found that SAHA treatment decreased α-SMA, YAP and TAZ proteins levels statistically. Conclusion: Decreased cell viability could be due to physiological, autophagical and also related to the apop totical mechanisms. We thought that SAHA plays an important role in the creation of the fates of the LX2 cell line.
AU  - Başkol, Mevlüt
AU  - KILIÇ, Eser
AU  - HELVACI, Nazlı
AU  - GÜÇLÜ, Kenan
AU  - baskol, gulden
AU  - Özel, Merve
DO  - 10.1515/tjb-2019-0397
PY  - 2021
JO  - Türk Biyokimya Dergisi
VL  - 46
IS  - 3
SN  - 1303-829X
SP  - 299
EP  - 305
DB  - TRDizin
UR  - http://search/yayin/detay/492543
ER  -