The role of the delta neutrophil index in determining the etiology of neonatal sepsis

SANCAK, Banu; YURDAKÖK, Murat; ÇELİK, Hasan Tolga; PORTAKAL, Oytun; BÜYÜKEREN, MELEK; yigit, sule

doi:10.1515/tjb-2020-0030

The role of the delta neutrophil index in determining the etiology of neonatal sepsis

Melek BÜYÜKEREN, (Hacettepe Üniversitesi, Tıp Fakültesi, Pediatri Bölümü, Neonatoloji Anabilim Dalı, Ankara, Türkiye)

Hasan Tolga ÇELİK, (Hacettepe Üniversitesi, Tıp Fakültesi, Pediatri Bölümü, Neonatoloji Anabilim Dalı, Ankara, Türkiye)

Şule YİĞİT, (Hacettepe Üniversitesi, Tıp Fakültesi, Pediatri Bölümü, Neonatoloji Anabilim Dalı, Ankara, Türkiye)

Oytun PORTAKAL, (Hacettepe Üniversitesi, Tıp Fakültesi, Biyokimya Bölümü, Ankara, Türkiye)

Banu SANCAK, (Hacettepe Üniversitesi, Tıp Fakültesi, Klinik Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)

Murat YURDAKÖK (Hacettepe Üniversitesi, Tıp Fakültesi, Pediatri Bölümü, Neonatoloji Anabilim Dalı, Ankara, Türkiye)

Türk Biyokimya Dergisi

2 0

Yıl: 2021 Cilt: 46 Sayı: 1 Sayfa Aralığı: 73 - 79 Metin Dili: İngilizce DOI: 10.1515/tjb-2020-0030 İndeks Tarihi: 21-02-2022

The role of the delta neutrophil index in determining the etiology of neonatal sepsis

Öz:

Objectives: To demonstrate immature granulocyte (IG) count and delta neutrophil index (DNI) values (novel po tential predictive marker for neonatal sepsis) for neonates. Methods: This prospective controlled clinical study was con sisted of 208 patients (77 in the study group and 131 in the control group) who were delivered between January 2016 and January 2018 at the Hacettepe University Neonatal Intensive CareUnitinAnkara, Turkey. In this study, we evaluated value of DNIin diagnosing neonatal sepsis by comparing the DNI values in culture positive septic neonates with healthy neonates. Results: In our study, the median interquartile range (IQR = 25–75%) DNI was 0.1% (0.0–0.3%) in the control group and 1.5% (1.0–2.45%) in the sepsis group (p < 0.05). In our ROC curve analysis, the cut-off value for the DNI as a sepsis marker was 0.65%, with 96.2% specificity and 97.4% sensitivity. Those patients with gram-negative isolates had significantly higher DNI and IG counts when compared to those patients with gram-positive bacteria (p < 0.05). Conclusions: Our findings indicated that the DNI counts are significant diagnostic biomarkers for neonatal sepsis. They may also have utility in determining the sepsis eti ology (differentiating between gram-positive and gram negative agents).

Anahtar Kelime:

Neonatal sepsis etiolojisinin belirlenmesinde delta nötrofil indeksinin rolü

Öz:

Amaç: Yenidoğanlarda olgunlaşmamış granülosit (IG) sayısı ve delta nötrofil indeks (DNI) değerlerini (yenido ğan sepsisi için yeni potansiyel prediktif belirteç) göstermek. Gereç ve Yöntem: Bu prospektif kontrollü klinik çalış mada, Ocak 2016–Ocak 2018 tarihleri arasında Hacettepe Üniversitesi Yenidoğan Yoğun Bakım Ünitesinde yatan 208 hasta (çalışma grubunda 77 ve kontrol grubunda 131) değerlendirildi. Bu çalışmada, kültür kanıtlı sepsis tanısı alan yenidoğanların DNI değerlerini, sağlıklı yenido ğanlarla karşılaştırarak; neonatal sepsis tanısı için DNI ölçümlerini değerlendirdik. Bulgular: Çalışmamızda, DNI değerleri; ortanca (% 25– 75 persentil) kontrol grubunda% 0.1 (% 0.0−0.3) ve sepsis grubunda % 1.5 (% 1.0−2.45) idi (p < 0.05). ROC eğrisi analizinde, sepsis markeri olarak DNI için cut-off değeri % 0.65 (% 96.2 özgüllük ve % 97.4 duyarlılık) idi. Gram-negatif bakteriler ile sepsis olan hastalar, gram pozitif bakteriler ile sepsis olan hastalara kıyasla önemli ölçüde daha yüksek DNI ve IG sayılarına sahipti (p < 0.05). Sonuç: Bulgularımız DNI değerinin yenidoğan sepsisi için önemli bir tanısal biyobelirteç olabileceğini göstermiştir. Ayrıca sepsis etiyolojisinin belirlenmesinde de faydalı olabilir (Gram-pozitif ve gram-negatif etkenler arasında ayrım yapabilir).

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BÜYÜKEREN M, ÇELİK H, yigit s, PORTAKAL O, SANCAK B, YURDAKÖK M (2021). The role of the delta neutrophil index in determining the etiology of neonatal sepsis. , 73 - 79. 10.1515/tjb-2020-0030
Chicago	BÜYÜKEREN MELEK,ÇELİK Hasan Tolga,yigit sule,PORTAKAL Oytun,SANCAK Banu,YURDAKÖK Murat The role of the delta neutrophil index in determining the etiology of neonatal sepsis. (2021): 73 - 79. 10.1515/tjb-2020-0030
MLA	BÜYÜKEREN MELEK,ÇELİK Hasan Tolga,yigit sule,PORTAKAL Oytun,SANCAK Banu,YURDAKÖK Murat The role of the delta neutrophil index in determining the etiology of neonatal sepsis. , 2021, ss.73 - 79. 10.1515/tjb-2020-0030
AMA	BÜYÜKEREN M,ÇELİK H,yigit s,PORTAKAL O,SANCAK B,YURDAKÖK M The role of the delta neutrophil index in determining the etiology of neonatal sepsis. . 2021; 73 - 79. 10.1515/tjb-2020-0030
Vancouver	BÜYÜKEREN M,ÇELİK H,yigit s,PORTAKAL O,SANCAK B,YURDAKÖK M The role of the delta neutrophil index in determining the etiology of neonatal sepsis. . 2021; 73 - 79. 10.1515/tjb-2020-0030
IEEE	BÜYÜKEREN M,ÇELİK H,yigit s,PORTAKAL O,SANCAK B,YURDAKÖK M "The role of the delta neutrophil index in determining the etiology of neonatal sepsis." , ss.73 - 79, 2021. 10.1515/tjb-2020-0030
ISNAD	BÜYÜKEREN, MELEK vd. "The role of the delta neutrophil index in determining the etiology of neonatal sepsis". (2021), 73-79. https://doi.org/10.1515/tjb-2020-0030

APA	BÜYÜKEREN M, ÇELİK H, yigit s, PORTAKAL O, SANCAK B, YURDAKÖK M (2021). The role of the delta neutrophil index in determining the etiology of neonatal sepsis. Türk Biyokimya Dergisi, 46(1), 73 - 79. 10.1515/tjb-2020-0030
Chicago	BÜYÜKEREN MELEK,ÇELİK Hasan Tolga,yigit sule,PORTAKAL Oytun,SANCAK Banu,YURDAKÖK Murat The role of the delta neutrophil index in determining the etiology of neonatal sepsis. Türk Biyokimya Dergisi 46, no.1 (2021): 73 - 79. 10.1515/tjb-2020-0030
MLA	BÜYÜKEREN MELEK,ÇELİK Hasan Tolga,yigit sule,PORTAKAL Oytun,SANCAK Banu,YURDAKÖK Murat The role of the delta neutrophil index in determining the etiology of neonatal sepsis. Türk Biyokimya Dergisi, vol.46, no.1, 2021, ss.73 - 79. 10.1515/tjb-2020-0030
AMA	BÜYÜKEREN M,ÇELİK H,yigit s,PORTAKAL O,SANCAK B,YURDAKÖK M The role of the delta neutrophil index in determining the etiology of neonatal sepsis. Türk Biyokimya Dergisi. 2021; 46(1): 73 - 79. 10.1515/tjb-2020-0030
Vancouver	BÜYÜKEREN M,ÇELİK H,yigit s,PORTAKAL O,SANCAK B,YURDAKÖK M The role of the delta neutrophil index in determining the etiology of neonatal sepsis. Türk Biyokimya Dergisi. 2021; 46(1): 73 - 79. 10.1515/tjb-2020-0030
IEEE	BÜYÜKEREN M,ÇELİK H,yigit s,PORTAKAL O,SANCAK B,YURDAKÖK M "The role of the delta neutrophil index in determining the etiology of neonatal sepsis." Türk Biyokimya Dergisi, 46, ss.73 - 79, 2021. 10.1515/tjb-2020-0030
ISNAD	BÜYÜKEREN, MELEK vd. "The role of the delta neutrophil index in determining the etiology of neonatal sepsis". Türk Biyokimya Dergisi 46/1 (2021), 73-79. https://doi.org/10.1515/tjb-2020-0030