Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae)

Emine DUMAN ERBAŞ, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)
Hülya ALTUNTAŞ (Eskişehir Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)
Karadeniz Fen Bilimleri Dergisi
2 0
Yıl: 2021 Cilt: 11 Sayı: 1 Sayfa Aralığı: 18 - 28 Metin Dili: İngilizce DOI: 10.31466/kfbd.834252 İndeks Tarihi: 29-07-2022

Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae)

Öz:
Reducing the usage of chemical pesticides used to control pests in agriculture and stored products is an important phenomenon. Also, eco-friendly biopesticides have become highly important for use in the management of the pest control programs to overcome the negative influences of other synthetic chemicals. In this study oxidative stress changes induced by one important phytochemical compound, juglone, in Galleria mellonella larvae were investigated. For this purpose, effective concentrations (LC10: 0.5 mg; LC30: 1.5 mg; LC50: 2.3 mg) of juglone were added into the 2 g diet of the first instar larvae. Hemolymph samples from the last instar larvae were used to analyses of antioxidant enzyme activity (glutathione peroxidases – GPx, glutathione-S transferase - GST, catalase - CAT, and superoxide dismutase - SOD) and malondialdehyde (MDA) assays. Activities of antioxidant enzymes (SOD, CAT, and GST) and MDA level in the hemolymph of last instar G. mellonella larvae exposed to dietary juglone changed dose-dependently when compared to the control larvae. However, there was no change detected in GPx enzyme activity in both treated and untreated larvae. Consequently, effective doses of juglone are toxic to the model and pest insect G. mellonella.
Anahtar Kelime:

Juglonun Büyük Balmumu Güvesi Galleria mellonella L. (Lepidoptera: Pyrelidae)’nın Larval Hemolenfinde Antioksidan Metabolizma Üzerine Etkileri

Öz:
Tarımsal alanlarda ve depolanan ürünlerde zararlı böcekler ile mücadele amacıyla kullanılan kimyasal pestisitlerin uygulamalarının azaltılması önemli bir olgudur. Bununla birlikte, çevre dostu biyopestisitler zararlı kontrol programlarının yönetiminde diğer sentetik kimyasalların olumsuz etkilerinin üstesinden gelmek için önemli hale gelmiştir. Bu çalışmada, önemli bir fitokimyasal bileşik olan Juglonun Galleria mellonella larvalarında neden olduğu oksidatif stres değişiklikleri incelenmiştir. Bu amaçla, juglonun etkili konsantrasyonları (LC10: 0.5 mg; LC30: 1.5 mg; LC50: 2.3 mg) birinci evre larvaların 2 gram besini içerisine ilave edildi. Son dönem larvaların hemolenf örnekleri, antioksidan enzim aktivitesi (glutatyon peroksidazlar - GPx, glutatyon-S transferaz - GST, katalaz - CAT ve süperoksit dismutaz - SOD) ve malondialdehit (MDA) testlerinin analizlerinde kullanıldı. Besinsel juglona maruz kalan son dönem G. mellonella larvalarının hemolenfinde antioksidan enzim aktiviteleri (SOD, CAT ve GST) ve MDA miktarı kontrol larvalarıyla karşılaştırıldığında doza bağlı olarak değişmiştir. Bununla birlikte, uygulama yapılan ve yapılmayan larvalara ait GPx enzim aktivitesinde değişiklik belirlenmedi. Sonuç olarak, juglonun etkili dozları model ve zararlı böcek G. mellonella için toksiktir.
Anahtar Kelime: Galleria mellonella

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Akhtar, Y., Isman, M. B., Niehaus, L. A., Lee, C. H. and Lee, H. S. (2012). Antifeedant and toxic effects of naturally occurring and synthetic quinones to the cabbage looper, Trichoplusia ni. Crop Protection, 31, 8-14.
  • Altuntaş, H. (2015a). Determination of gibberellic acid (GA3)-induced oxidative stress in a model organism Galleria mellonella L. (Lepidoptera: Pyralidae). Environmental Entomology (Physiology, 44 (1), 100- 105.
  • Altuntaş, H. (2015b). Effects of ethephon on the hemolymph metabolites of the greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae). Acta Physica Polonica A, 128, 182-183.
  • Altuntaş, H., Duman, E. and Kılıç, G. (2020). Juglone induced oxidative and genotoxic stress in the model insect Galleria mellonella L. (Pyralidae: Lepidoptera). International Journal of Tropical Insect Science, 40, 611-619.
  • Altuntaş, H., Duman, E., Şanal-Demirci, S. N. and Ergin, E. (2016). Toxicological and physiological effects of ethephon on the model organism, Galleria mellonella L. 1758 (Lepidoptera: Pyralidae). Türkiye Entomoloji Dergisi, 40 (4), 413-423.
  • Aslantürk, A., Kalender, S., Uzunhisarcıklı, M. and Kalender, Y. (2011). Effects of methidathion on antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae. Journal of the Entomological Research Society, 13 (3), 27-38.
  • Babula, P., Adam, V., Havel, L. and & Kizek, R. (2009). Noteworthy Secondary Metabolites Naphthoquinones - their Occurrence, Pharmacological Properties and Analysis. Current Pharmaceutical Analysis, 5, 47- 68.
  • Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principal of protein-dye binding. Annu. Biochem., 72, 248-254.
  • Büyükgüzel, E., Büyükgüzel, K., Snela, M., Erdem, M., Radtke, K., Ziemnicki, K. and Adamski, Z. (2013). Effect of boric acid on antioxidant enzyme activity, lipid peroxidation and ultrastructure of midgut and fat body of Galleria mellonella. Cell Biology and Toxicology, 29, 117-129.
  • Büyükgüzel, E., Hyršl, P. and Büyükgüzel, K. (2010). Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 156 (2),176-183.
  • Cespedes, C. L., Lina-Garcia, L., Kubo, I., Salazar, J. R., Ariza-Castolo, A., Alarcon, J., Aqueveque, P., Werner, E. and Seigler, D. S. (2016). Calceolaria integrifolia s. l. complex, reduces feeding and growth of Acanthoscelides obtectus, and Epilachna varivestis. A new source of bioactive compounds against dry bean pests. Industrial Crops & Products, 89, 257-267.
  • Chance, B. and Maehly, A. C. (1955). Assay of catalase and peroxidases. Methods Enzymology, 2, 764-775.
  • Duman-Erbaş, E. and Altuntaş, H. (2020). Lethal and Sublethal Effects of Juglone on the Life-History Traits of Galleria mellonella L. (Lepidoptera: Pyralidae). Acta Zool. Bulg., 72 (1), 43-48.
  • Emam, A. M., Swelam, E. S. and Megally, N. Y. (2009). Furocoumarin and quinoloune alkaloid with larvicidal and antifeedant activities isolated from Ruta chalepensis leaves. Journal of Natural Products, 2, 10-22.
  • Felton, G. W. and Duffey, S. S. (1991). Protective action of midgut catalase in lepidopteran larvae against oxidative plant defenses. Journal of Chemical Ecology, 17, 1715-1732.
  • Felton, G. W. and Summers, C. B. (1995). Antioxidant systems in insects. Archives of Insects Biochemistry and Physiology, 29 (2), 187-197.
  • Felton, G. W., Donato, K. K., Del-Vecchio, R. J. and Duffey, S. S. (1989). Activation of plant foliar oxidases by insect feeding reduces nutritive quality of foliage for noctuid herbivores. Journal of Chemical Ecology, 15, 2667-2694.
  • Gaeta, L. M., Tozzi, G., Pastore, A., Federici, G. and Piemonte, F. (2002). Determination of superoxide dismutase and glutathione peroxidase activities in blood of healthy pediatric subjects. Clinica Chimica Acta, 322, 117-120.
  • Hu, W., Du, W., Bai, S., Lv, S. and Chen, G. (2017). Phenoloxidase, an effective bioactivity target for botanical insecticide screening from green walnut husks. Nat Prod Res.
  • Islam, A. K. M. M. and Widhalm, J. R. (2020). Agricultural Uses of Juglone: Opportunities and Challenges. Agronomy, 10, 1500.
  • Jeyasankar, A., Raja, N. and Ignacimuthu, S. (2010). Antifeedant and growth inhibitory activities of Syzygium lineare Wall (Myrtaceae) against Spodoptera litura Fab (Lepidoptera: Noctuidae). Current Research Journal of Biological Sciences, 2 (3), 173-177.
  • Kazzaz, J. A., Xu, J., Palaia, T. A., Mantell, L., Fein, A. M. and Stuart-Horowitz, S. (1996). Cellular Oxygen Toxicity. The Journal of Biological Chemistry, 271 (25), 15182-15186.
  • Krishnan, N. and Kodrik, D. (2006). Antioxidant enzymes in Spodoptera littoralis (Boisduval): Are they enhanced to protect gut tissues during oxidative stres. Journal of Insect Physiology, 52, 11-20.
  • Lee, K. (1991). Glutathione S-transferase activities in phytophagous insects: Induction and inhibition by plant phototoxins and phenols. Insect Biochemistry, 21, 353.
  • Lindroth, R. L., Anson, B. D. and Weisbrod, A. V. (1990). Effects of protein and Juglone on Gypsy moths: Growth performance and detoxification enzyme activity. Journal of Chemical Ecology, 16 (8), 2533- 2547.
  • Lv, S. T., Du, X., Bai, S. M. and Chen, G. (2018). Insecticidal effect of juglone and its disturbance analysis in metabolic profiles of Aphis gossypii glover using 1 H NMR-based metabonomics approach. Phytoparasitica, 46, 521-531.
  • Paul, D. and Sohkhlet, M. D. (2012). Anti-feedant, repellent and growth regulatory effects of four plants extracts on Pieris brassiceae larvae (Lepidoptera: Pieridae). Open Access Scientific Reports, 1 (10), 1- 5.
  • Peric´-Mataruga, V., Blagojevic, D., Spasic, M. B., Ivanovic, J. and Jankovic-Hladni, M. (1997). Effect of the host plant on the antioxidative defence in the midgut of Lymantria dispar L. caterpillars of different population origins. Journal of Insect Physiology, 43, 101-106.
  • Piskorski, R., Ineichen, S. and Dorn, S. (2011). Ability of the oriental fruit moth Grapholita molesta (Lepidoptera: Tortricidae) to detoxify Juglone, the main secondary metabolite of the non-host plant walnut. Journal of Chemical Ecology, 37, 1110-1116.
  • Rattan, R. S. and Sharma, A. (2011). Plant secondary metabolites in the sustainable diamondback moth (Plutella xylostella L.) management. Indian Journal of Fundamental and Applied Life Sciences, 1 (3), 295-309.
  • Sankarapandi, S. and Zweier, J. L. (1999). Evidence against the Generation of Free Hydroxyl Radicals from the Interaction of Copper, Zinc-Superoxide Dismutase and Hydrogen Peroxide. The Journal of Biological Chemistry, 274 (49), 34576-34583.
  • Scheline, R. R. (1978). Mammalian metabolism of plant xenobitics. Academic Press, New York. Sezer-Tuncsoy, B., Tuncsoy, M., Gomes, T., Sousa, V., Teixeira, M. R., Bebianno, M. J. and Ozalp, P. (2019). Effects of Copper Oxide Nanoparticles on Tissue Accumulation and Antioxidant Enzymes of Galleria mellonella L. Bulletin of Environmental Contamination and Toxicology, 102, 341-346.
  • Sorokin, N. and Whitaker, J. (2008). The impacts of selected natural plant chemicals on terrestrial invertebrates. Secondary Metabolites in Soil Ecology Chapter 12 (Ed) Karlovsky P, Berlin, Springer.
  • Sun, M., Wang, Y., Song, Z. and Fang, G. (2007). Insecticidal activities and active components of the alcohol extract from green peel of Juglans mandshurica. Journal of Forestry Research, 18, 62-64.
  • Şeref-Gün, S., Çinbilgel, İ., Öz, E. and Çetin, H. (2011). Bazı Salvia L. (Labiatae) bitki ekstraktlarının, sivrisinek Culex pipiens L. (Diptera: Culicidae)’e karşı larva öldürücü aktivitesi. Kafkas Üniversitesi Veterinerlik Fakültesi Dergisi, 17 (Suppl A), 61-65.
  • Tan, S., Sagara, Y., Liu, Y., Maher, P. and Schubert, D. (1998). The Regulation of Reactive Oxygen Species Production during Programmed Cell Death. The Journal of Cell Biology, 141, 1423-1432.
  • Thiboldeaux, R. L., Lindroth, R. L. and Tracy, J. W. (1994). Differential toxicity of juglone (5-hydroxy-1,4- naphthoquinone) and related naphthoquinones to Saturniid moths. Journal of Chemical Ecology, 20, 1631-1641.
  • Thiboldeaux, R. L., Lindroth, R. L. and Tracy, J. W. (1998). Effects of juglone (5-hydroxy-1,4- naphthoquinone) on midgut morphology and glutathione status in Saturniid moth larvae. Comparative Biochemistry and Physiology, 120, 481-487.
  • Wadleigh, R. W. and Yu, S. J. (1988). Detoxcification of ısothiocyanate allelochemicals by glutathione transferase in three lepidopteraus species. Journal of Chemical Ecology, 14, 1279-1288.
  • Wheeler, G. S., Slansky, F. and Yu, S. J. (1993). Fall armyworm sensitivity to flavone: Limited role of constitutive and induced detoxifying enzyme activity. Journal of Chemical Ecology, 19, 645.
  • Yu, S. J. (1982). Host plant induction of glutathione-S-transferase in the faal armyworm. Pesticide Biochemistry and Physiology, 18, 101-106.
  • Yu, S. J. (1987). Quinone reductase of phytophagous insects and its induction by allelochcmicals. Comparative Biochemistry and Physiology, 87B, 621-624.
  • Yu, S. J. and Abo-Elghar, G. E. (2000). Allelochemicals as Inhibitors of Glutathione S-Transferases in the Fall Armyworm. Pesticide Biochemistry and Physiology, 68, 173-183.
  • Yu, S. J. and Huang, S. W. (2000). Purification and characterization of glutathione S-transferases from the German cockroach, Blattella germanica (L.). Pesticide Biochemistry and Physiology, 67, 36.
APA DUMAN ERBAS E, ALTUNTAS H (2021). Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae). , 18 - 28. 10.31466/kfbd.834252
Chicago DUMAN ERBAS EMINE,ALTUNTAS HULYA Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae). (2021): 18 - 28. 10.31466/kfbd.834252
MLA DUMAN ERBAS EMINE,ALTUNTAS HULYA Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae). , 2021, ss.18 - 28. 10.31466/kfbd.834252
AMA DUMAN ERBAS E,ALTUNTAS H Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae). . 2021; 18 - 28. 10.31466/kfbd.834252
Vancouver DUMAN ERBAS E,ALTUNTAS H Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae). . 2021; 18 - 28. 10.31466/kfbd.834252
IEEE DUMAN ERBAS E,ALTUNTAS H "Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae)." , ss.18 - 28, 2021. 10.31466/kfbd.834252
ISNAD DUMAN ERBAS, EMINE - ALTUNTAS, HULYA. "Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae)". (2021), 18-28. https://doi.org/10.31466/kfbd.834252
APA DUMAN ERBAS E, ALTUNTAS H (2021). Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae). Karadeniz Fen Bilimleri Dergisi, 11(1), 18 - 28. 10.31466/kfbd.834252
Chicago DUMAN ERBAS EMINE,ALTUNTAS HULYA Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae). Karadeniz Fen Bilimleri Dergisi 11, no.1 (2021): 18 - 28. 10.31466/kfbd.834252
MLA DUMAN ERBAS EMINE,ALTUNTAS HULYA Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae). Karadeniz Fen Bilimleri Dergisi, vol.11, no.1, 2021, ss.18 - 28. 10.31466/kfbd.834252
AMA DUMAN ERBAS E,ALTUNTAS H Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae). Karadeniz Fen Bilimleri Dergisi. 2021; 11(1): 18 - 28. 10.31466/kfbd.834252
Vancouver DUMAN ERBAS E,ALTUNTAS H Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae). Karadeniz Fen Bilimleri Dergisi. 2021; 11(1): 18 - 28. 10.31466/kfbd.834252
IEEE DUMAN ERBAS E,ALTUNTAS H "Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae)." Karadeniz Fen Bilimleri Dergisi, 11, ss.18 - 28, 2021. 10.31466/kfbd.834252
ISNAD DUMAN ERBAS, EMINE - ALTUNTAS, HULYA. "Effects of Juglone on the Antioxidant Metabolism in the Larval Hemolymph of the Greater Wax Moth Galleria mellonella L. (Lepidoptera: Pyralidae)". Karadeniz Fen Bilimleri Dergisi 11/1 (2021), 18-28. https://doi.org/10.31466/kfbd.834252
Sayfa Oluşturulma Tarihi
17-04-2024 23:36

İLETİŞİM

TÜBİTAK ULAKBİM TR Dizin

Yüzüncüyıl, İşçi Blokları Mahallesi

Muhsin Yazıcıoğlu Caddesi No:51/C

06530 Çankaya / ANKARA +90 (312) 298 92 00

trdizin@tubitak.gov.tr

TÜBİTAK ULAKBİM Ulusal Akademik Ağ ve Bilgi Merkezi Cahit Arf Bilgi Merkezi © Tüm Hakları Saklıdır. Gizlilik Politikası Kullanım Şartları