EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES

doğan, nurcan; doğan, cemhan

doi:10.23902/trkjnat.960073

EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES

Nurcan DOĞAN, (Bozok Üniversitesi, Boğazlıyan Meslek Yüksekokulu, Gıda Teknolojisi Bölümü, Yozgat, Türkiye)

Cemhan DOĞAN (Bozok Üniversitesi, Boğazlıyan Meslek Yüksekokulu, Gıda Teknolojisi Bölümü, Yozgat, Türkiye)

Trakya University Journal of Natural Sciences

3 1

Yıl: 2021 Cilt: 22 Sayı: 2 Sayfa Aralığı: 245 - 253 Metin Dili: İngilizce DOI: 10.23902/trkjnat.960073 İndeks Tarihi: 29-07-2022

EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES

Öz:

The possible side effects of drugs used in type II diabetes are increasing the tendency to herbal resources that have been used for many years. Senecio vernalis Waldst. & Kit is one of the annual Senecio L. species widely distributed in Turkey and used as a food and folk medicine. In this study, optimization of extraction conditions on the bioactive properties (Total phenolic content (TPC) and antioxidant capacity) of the flowers of S. vernalis and the potential of the plant for α-amylase, α-glucosidase, and lipase inhibitory activity were investigated. The optimum extraction conditions were determined at 69.72% water concentration, 59℃ for 26.15 min, and the highest experimental values of TPC and 2, 2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate (DPPH) scavenging activity were observed as 28.14 mg gallic acid equivalent (GAE) g-1 and 3165.99 mg trolox equivalent (TE)/100 g sample, respectively. Significant inhibition was observed for α-amylase and α-glucosidase which are the key enzymes in type II diabetes, at a concentration of 100 mg mL-1, with 21.32% and 64.16% respectively. The S. vernalis extracts showed no detectable inhibition of lipase. The results showed that S. vernalis, which has high antioxidant capacity also has a significant anti-diabetic effect. It can be concluded that S. vernalis can be considered a natural resource in many industries such as food and pharmaceuticals.

Anahtar Kelime: Extraction optimization Type II diabetes Antioxidant activity Senecio vernalis

Öz:

Tip II diyabette kullanılan ilaçların olası yan etkileri, uzun yıllardır kullanılan bitkisel kaynaklara olan eğilimi arttırmaktadır. Senecio vernalis Waldst. & Kit, Türkiye'de yaygın olarak bulunan, gıda ve halk ilacı olarak kullanılan tek yıllık Senecio L. türlerinden biridir. Bu nedenle, bu çalışmada, S. vernalis çiçeklerinin biyoaktif özellikleri (Toplam fenolik madde miktarı (TPC) ve antioksidan kapasite) ve α-amilaz, α-glukozidaz ve lipaz inhibitör aktivite potansiyeli üzerinde optimizasyon ekstraksiyon koşulları araştırıldı. Optimum ekstraksiyon koşulları %69.72 su konsantrasyonunda, 59℃'de 26.15 dakika olarak belirlenmiş ve TPC ve 2, 2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate (DPPH) süpürme aktivitesinin en yüksek deneysel değerleri sırasıyla 28,14 mg gallik asit eşdeğeri (GAE) g-1 ve 3165.99 mg troloks eşdeğeri (TE)/100 g numune olarak belirlenmiştir. Tip II diyabette anahtar enzim olan α-amilaz, α-glukozidaz için 100 mg mL-1 konsantrasyonunda sırasıyla %21.32 ve %64.16 inhibisyon gözlemlendi. Senecio vernaris ekstraktı, saptanabilir bir lipaz inhibisyonu göstermedi. Sonuçlar, yüksek bir antioksidan kapasiteye sahip olan S. vernalis'in de önemli bir anti-diyabetik etkiye sahip olduğunu göstermiştir. Senecio vernalis'in gıda ve ilaç gibi birçok endüstride doğal bir kaynak olarak değerlendirilebileceği sonucuna varılabilir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	doğan n, doğan c (2021). EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES. , 245 - 253. 10.23902/trkjnat.960073
Chicago	doğan nurcan,doğan cemhan EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES. (2021): 245 - 253. 10.23902/trkjnat.960073
MLA	doğan nurcan,doğan cemhan EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES. , 2021, ss.245 - 253. 10.23902/trkjnat.960073
AMA	doğan n,doğan c EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES. . 2021; 245 - 253. 10.23902/trkjnat.960073
Vancouver	doğan n,doğan c EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES. . 2021; 245 - 253. 10.23902/trkjnat.960073
IEEE	doğan n,doğan c "EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES." , ss.245 - 253, 2021. 10.23902/trkjnat.960073
ISNAD	doğan, nurcan - doğan, cemhan. "EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES". (2021), 245-253. https://doi.org/10.23902/trkjnat.960073

APA	doğan n, doğan c (2021). EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES. Trakya University Journal of Natural Sciences, 22(2), 245 - 253. 10.23902/trkjnat.960073
Chicago	doğan nurcan,doğan cemhan EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES. Trakya University Journal of Natural Sciences 22, no.2 (2021): 245 - 253. 10.23902/trkjnat.960073
MLA	doğan nurcan,doğan cemhan EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES. Trakya University Journal of Natural Sciences, vol.22, no.2, 2021, ss.245 - 253. 10.23902/trkjnat.960073
AMA	doğan n,doğan c EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES. Trakya University Journal of Natural Sciences. 2021; 22(2): 245 - 253. 10.23902/trkjnat.960073
Vancouver	doğan n,doğan c EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES. Trakya University Journal of Natural Sciences. 2021; 22(2): 245 - 253. 10.23902/trkjnat.960073
IEEE	doğan n,doğan c "EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES." Trakya University Journal of Natural Sciences, 22, ss.245 - 253, 2021. 10.23902/trkjnat.960073
ISNAD	doğan, nurcan - doğan, cemhan. "EXTRACTION OPTIMIZATION OF Senecio vernalis Waldst. & Kit AND DETERMINATION OF ANTI-α-AMYLASE/α-GLUCOSIDASE, ANTI-LIPASE AND ANTIOXIDANT ACTIVITIES". Trakya University Journal of Natural Sciences 22/2 (2021), 245-253. https://doi.org/10.23902/trkjnat.960073