In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability
Yıl: 2021 Cilt: 16 Sayı: 2 Sayfa Aralığı: 182 - 188 Metin Dili: İngilizce DOI: 10.17094/ataunivbd.842103 İndeks Tarihi: 29-07-2022
In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability
Öz: Neuroblastoma (SH-SY5Y) is one of the most common solid tumors in children, with aggressive and resistant features. Valproic acid (VPA) is a histone deacetylase inhibitor (HDAC) and is thought to have antitumoral activity. The aim of the current study is the evaluation of valproic acid and cisplatin (CSP) combination antitumor effects on SH-SY5Y. For this aim, the different doses of cisplatin (5, 10, and 15 μg/ml), VPA (5mM), and CSP (5, 10 and 15 μg/ml) + VPA (5 mM) were applied on SH-SY5Y tumor cell culture for 24 hours. For evaluation of cell viability, apoptosis, antioxidant and oxidant status, 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT), Anexin-V-FITC apoptosis, Total Antioxidant Capacity (TAC) and Total Oxidant Status (TOS) tests were done 24 hours after drug administration. According to our results, the combination of 15 μg/ml cisplatin and 5 mM VPA reduced cell viability and increased apoptosis status, compared with the negative control group (P<0.05). Our studies showed valproic acid increased the cisplatin effect and reduced the viability of tumor cells. The combination of VPA + CSP is more effective than only using cisplatin. In the light of these data, it is thought that the efficacy of VPA + CSP combined therapy should be investigated more comprehensively with in vivo studies.
Anahtar Kelime: Valproik Asit ve Cisplatin Kombinasyonunun Nöroblastom Tümörlerinde Canlılık Üzerine Antitümör Etkisinin İn Vitro Olarak Belirlenmesi
Öz: Nöroblastoma (SH-SY5Y), agresif ve dirençli özellikleri ile çocuklarda en sık görülen solid tümörlerden biridir. Valproik asit (VPA) bir histon deasetilaz inhibitörüdür (HDAC) ve antitümoral aktiviteye sahip olduğu düşünülmektedir. Bu çalışmanın amacı valproik asit ve sisplatin (CSP) kombinasyonun SH-SY5Y üzerindeki antitümör etkilerinin değerlendirilmesidir. Bu amaçla farklı dozlarda sisplatin (5, 10 ve 15 μg / ml), VPA (5mM) ve CSP (5, 10 ve 15 μg / ml) + VPA (5 mM) SH-SY5Y'ye hücre hattına 24 saat boyunca uygulanmıştır. Hücre canlılığı, apoptoz, antioksidan ve oksidan durumunun değerlendirilmesi için, 3- (4,5-Dimetiltiyazol-2-Yl) -2,5-Difeniltetrazolyum Bromür (MTT), Anexin-V-FITC apoptoz, Toplam Antioksidan Kapasite (TAC) ve Toplam Oksidan Durum (TOS) testleri ilaç uygulamasından 24 saat sonra yapılmıştır. Sonuçlarımıza göre, 15 μg / ml sisplatin ve 5 mM VPA kombinasyonu, negatif kontrol grubuna kıyasla hücre canlılığını azalttığı ve apoptoz durumunu arttırdığı tespit edilmiştir (P<0.05). Çalışmamızın sonuçları, valproik asidin cisplatin etkisini artırdığını ve kanser hücrelerinin canlılığını azalttığını ve VPA + CSP kombinasyonunun tek başına sispilatin uygulamasına kıyasla daha etkili olduğunu göstermektedir. Bu veriler ışığında VPA + CSP kombine tedavi etkinliği in vivo çalışmalar ile daha geniş kapsamlı araştırılması gerektiği düşünülmektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | TAGHİZADEHGHALEHJOUGHİ A, Genç S, Yeni Y, Hacimuftuoglu A (2021). In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability. , 182 - 188. 10.17094/ataunivbd.842103 |
| Chicago | TAGHİZADEHGHALEHJOUGHİ Ali,Genç Sıdıka,Yeni Yeşim,Hacimuftuoglu Ahmet In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability. (2021): 182 - 188. 10.17094/ataunivbd.842103 |
| MLA | TAGHİZADEHGHALEHJOUGHİ Ali,Genç Sıdıka,Yeni Yeşim,Hacimuftuoglu Ahmet In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability. , 2021, ss.182 - 188. 10.17094/ataunivbd.842103 |
| AMA | TAGHİZADEHGHALEHJOUGHİ A,Genç S,Yeni Y,Hacimuftuoglu A In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability. . 2021; 182 - 188. 10.17094/ataunivbd.842103 |
| Vancouver | TAGHİZADEHGHALEHJOUGHİ A,Genç S,Yeni Y,Hacimuftuoglu A In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability. . 2021; 182 - 188. 10.17094/ataunivbd.842103 |
| IEEE | TAGHİZADEHGHALEHJOUGHİ A,Genç S,Yeni Y,Hacimuftuoglu A "In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability." , ss.182 - 188, 2021. 10.17094/ataunivbd.842103 |
| ISNAD | TAGHİZADEHGHALEHJOUGHİ, Ali vd. "In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability". (2021), 182-188. https://doi.org/10.17094/ataunivbd.842103 |
| APA | TAGHİZADEHGHALEHJOUGHİ A, Genç S, Yeni Y, Hacimuftuoglu A (2021). In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 16(2), 182 - 188. 10.17094/ataunivbd.842103 |
| Chicago | TAGHİZADEHGHALEHJOUGHİ Ali,Genç Sıdıka,Yeni Yeşim,Hacimuftuoglu Ahmet In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16, no.2 (2021): 182 - 188. 10.17094/ataunivbd.842103 |
| MLA | TAGHİZADEHGHALEHJOUGHİ Ali,Genç Sıdıka,Yeni Yeşim,Hacimuftuoglu Ahmet In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol.16, no.2, 2021, ss.182 - 188. 10.17094/ataunivbd.842103 |
| AMA | TAGHİZADEHGHALEHJOUGHİ A,Genç S,Yeni Y,Hacimuftuoglu A In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2021; 16(2): 182 - 188. 10.17094/ataunivbd.842103 |
| Vancouver | TAGHİZADEHGHALEHJOUGHİ A,Genç S,Yeni Y,Hacimuftuoglu A In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2021; 16(2): 182 - 188. 10.17094/ataunivbd.842103 |
| IEEE | TAGHİZADEHGHALEHJOUGHİ A,Genç S,Yeni Y,Hacimuftuoglu A "In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability." Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 16, ss.182 - 188, 2021. 10.17094/ataunivbd.842103 |
| ISNAD | TAGHİZADEHGHALEHJOUGHİ, Ali vd. "In Vitro Determination of Valproic Acid and Cisplatin Combination Antitumor Effect on Neuroblastoma Tumors Viability". Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16/2 (2021), 182-188. https://doi.org/10.17094/ataunivbd.842103 |
