Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats

Öz:

This study aimed to investigate the protective effects of Naringin in Cyclophosphamide (CYP)-induced cardiotoxicity in rats. In the study, forty male adult Sprague Dawley rats weighing approximately 200-250 g were used. Rats were divided into 5 experimental groups as control, CYP, Naringin50+CYP, Naringin100+CYP and Naringin100. Control and CYP groups were administered to intragastric (i.g.) saline for 10 days. Also, the CYP group was given a single dose of CYP (200 mg/kg, intraperitoneal (i.p.)) on the 10th day. Naringin50+CYP and Naringin100+CYP groups were administered i.g. 50 and 100 mg/kg doses of Naringin for 10 days, respectively, and was given a single dose of CYP (200mg/kg, intraperitoneal (i.p.)) on the 10th day. Naringin100 group was administered to Naringin (100mg/kg), i.g.) for 10 days. ECG was recorded under anesthesia, and intracardiac blood samples were taken. Troponin I, creatine kinase (CK) and creatine kinase-MB (CK-MB) parameters were examined in serum samples. As a result of the necropsy, preparations were prepared by routine cardiac tissue follow-up method for histopathological examination. For the detection of DNA damage in the cardiac tissue, 8-OHdG expression was investigated. CYP has been shown to have cardiotoxic effects on ECG values, cardiac enzymes, and cardiac histopathology of rats. In this study, it was determined that Naringin has a protective effect in CYP-induced cardiotoxicity in rats.

Anahtar Kelime: Cardiac enzymes ECG Naringin cyclophosphamide

Sıçanlarda Siklofosfamid ile İndüklenen Kardiyotoksisitede EKG, Kardiyak Enzimler, Kardiyak Histopatoloji ve 8-OHdG Ekspresyonu Üzerine Naringin’in Protektif Etkilerinin Araştırılması

Öz:

Bu çalışmada, sıçanlarda Siklofosfamid (CYP) ile indüklenen kardiyotoksisitede Naringin'in koruyucu etkilerinin araştırılması amaçlandı. Çalışmada, ortalama 200-250 gr ağırlığında kırk adet erkek yetişkin Sprague Dawley sıçan kullanıldı. Sıçanlar kontrol, CYP, Naringin 50+CYP, Naringin100+CYP ve Naringin100 olmak üzere 5 deney grubuna ayrıldı. Kontrol ve CYP gruplarına, 10 gün intragastrik (i.g.) serum fizyolojik uygulandı. Ayrıca, CYP 10. gün tek doz CYP (200 mg/kg, intraperitoneal (i.p.)) verildi. Naringin 50+CYP ve Naringin100+CYP gruplarına, 10 gün boyunca sırasıyla 50 ve 100 mg/kg dozunda i.g. Naringin verildi ve 10. gün tek doz CYP (200 mg/kg, i.p.) verildi. Naringin100 grubuna ise, 10 gün 100 mg/kg dozunda i.g. Naringin verildi. Anestezi altında EKG kaydı yapıldı ve intrakardiyak kan örnekleri alındı. Serum örneklerinde Troponin I, kreatin kinaz (CK) ve kreatin kinaz-MB (CK-MB) parametreleri incelendi. Otopsi sonucunda histopatolojik inceleme için rutin kalp dokusu takip yöntemi ile preparatlar hazırlandı. Kalp dokusunda DNA hasarının tespiti için 8-OHdG ekspresyonu araştırıldı. CYP'nin sıçanların EKG değerleri, kardiyak enzimleri ve kardiyak histopatolojisi üzerinde kardiyotoksik etkileri olduğu gösterilmiştir. Bu çalışmada, sıçanlarda CYP ile indüklenen kardiyotoksisitede Naringin’in koruyucu etkilere sahip olduğu belirlendi.

Anahtar Kelime: Rat

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KOLİKPINAR Y, Dağ Y, Tekin S, yildirim S, Durukan Azman P, ATASEVER A, BOLAT M, LAÇİN B, GÖK M, Erol Polat Ö, Çınar A (2021). Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats. , 196 - 203. 10.17094/ataunivbd.870668
Chicago	KOLİKPINAR YAVUZ SELİM,Dağ Yusuf,Tekin Samet,yildirim Serkan,Durukan Azman Pelin,ATASEVER Aslıhan,BOLAT Merve,LAÇİN Burak Batuhan,GÖK Melahat,Erol Polat Özlem,Çınar Ali Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats. (2021): 196 - 203. 10.17094/ataunivbd.870668
MLA	KOLİKPINAR YAVUZ SELİM,Dağ Yusuf,Tekin Samet,yildirim Serkan,Durukan Azman Pelin,ATASEVER Aslıhan,BOLAT Merve,LAÇİN Burak Batuhan,GÖK Melahat,Erol Polat Özlem,Çınar Ali Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats. , 2021, ss.196 - 203. 10.17094/ataunivbd.870668
AMA	KOLİKPINAR Y,Dağ Y,Tekin S,yildirim S,Durukan Azman P,ATASEVER A,BOLAT M,LAÇİN B,GÖK M,Erol Polat Ö,Çınar A Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats. . 2021; 196 - 203. 10.17094/ataunivbd.870668
Vancouver	KOLİKPINAR Y,Dağ Y,Tekin S,yildirim S,Durukan Azman P,ATASEVER A,BOLAT M,LAÇİN B,GÖK M,Erol Polat Ö,Çınar A Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats. . 2021; 196 - 203. 10.17094/ataunivbd.870668
IEEE	KOLİKPINAR Y,Dağ Y,Tekin S,yildirim S,Durukan Azman P,ATASEVER A,BOLAT M,LAÇİN B,GÖK M,Erol Polat Ö,Çınar A "Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats." , ss.196 - 203, 2021. 10.17094/ataunivbd.870668
ISNAD	KOLİKPINAR, YAVUZ SELİM vd. "Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats". (2021), 196-203. https://doi.org/10.17094/ataunivbd.870668

APA	KOLİKPINAR Y, Dağ Y, Tekin S, yildirim S, Durukan Azman P, ATASEVER A, BOLAT M, LAÇİN B, GÖK M, Erol Polat Ö, Çınar A (2021). Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 16(2), 196 - 203. 10.17094/ataunivbd.870668
Chicago	KOLİKPINAR YAVUZ SELİM,Dağ Yusuf,Tekin Samet,yildirim Serkan,Durukan Azman Pelin,ATASEVER Aslıhan,BOLAT Merve,LAÇİN Burak Batuhan,GÖK Melahat,Erol Polat Özlem,Çınar Ali Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats. Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16, no.2 (2021): 196 - 203. 10.17094/ataunivbd.870668
MLA	KOLİKPINAR YAVUZ SELİM,Dağ Yusuf,Tekin Samet,yildirim Serkan,Durukan Azman Pelin,ATASEVER Aslıhan,BOLAT Merve,LAÇİN Burak Batuhan,GÖK Melahat,Erol Polat Özlem,Çınar Ali Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol.16, no.2, 2021, ss.196 - 203. 10.17094/ataunivbd.870668
AMA	KOLİKPINAR Y,Dağ Y,Tekin S,yildirim S,Durukan Azman P,ATASEVER A,BOLAT M,LAÇİN B,GÖK M,Erol Polat Ö,Çınar A Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2021; 16(2): 196 - 203. 10.17094/ataunivbd.870668
Vancouver	KOLİKPINAR Y,Dağ Y,Tekin S,yildirim S,Durukan Azman P,ATASEVER A,BOLAT M,LAÇİN B,GÖK M,Erol Polat Ö,Çınar A Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2021; 16(2): 196 - 203. 10.17094/ataunivbd.870668
IEEE	KOLİKPINAR Y,Dağ Y,Tekin S,yildirim S,Durukan Azman P,ATASEVER A,BOLAT M,LAÇİN B,GÖK M,Erol Polat Ö,Çınar A "Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats." Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 16, ss.196 - 203, 2021. 10.17094/ataunivbd.870668
ISNAD	KOLİKPINAR, YAVUZ SELİM vd. "Investigation of Protective Effects of Naringin on ECG, Cardiac Enzymes, Cardiac Histopathology and 8-OHdG Expression in Cyclophosphamide-Induced Cardiotoxicity in Rats". Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16/2 (2021), 196-203. https://doi.org/10.17094/ataunivbd.870668