TY  - JOUR
TI  - RATIONAL PREPARATION OF POWDER ANTHOCYANIN INCORPORATED UREASE TEST FOR DETECTION OF K. PNEUMONIAE AND P. MIRABILIS
AB  - In this present study, we developed Brassica oleracea var. Capitata (red cabbage) extract (RCE) powderincorporated colorimetric media for rapid, selective, sensitive detection of urease-postive bacteria, Klebsiella pneumoniae ATCC 10031 and Proteus mirabilis ATCC 25933. The major component of RCE is anthocyanin groups which are in charge of pH dependent color change. The ureas test contains certain concetration of anthocyanin, urea prepated in 0.01 M sodium phosphate buffer and sodium azide. The urea in the test was hydrolyzed by urease enzyme released from K. pneumoniae and P.mirabilis and produce ammonium (NH3) molecules which sequentially made the test media alkaline, caused deprotonation of anthocyanin and induced the color change from pink to green. Also, Escherichia coli ATCC 25922 was used a urease-negative control bacteria. The detection of K. pneumoniae and P. mirabilis was examined as a function of anthocyanin concentration and incubation time. The anthocyanin was used lyophilized powder form in the urease test to discard the in interference of free proton in liquid form owing to addition of hydrochloric acid (HCl). We also performed the digital image analysis, Delta E (ΔE) for quantitative confirmation of the visual results. It was observed that the results showed that the color change from pink to green in 150 and 210 minutes of incubaiton for K. pneumoniae and P. mirabilis, respectively. Here in, we successfully prepared anthocyanin-incorporated urease test and perform colorimetric detection of K. pneumoniae and P. mirabilis with naked eyes and digital image analysis.
AU  - Ildız, Nilay
AU  - ÇELİK, Çağla
AU  - Can Sezgin, Gülten
DO  - 10.34108/eujhs.925890
PY  - 2021
JO  - Sağlık Bilimleri Dergisi
VL  - 30
IS  - 2
SN  - 1018-3655
SP  - 103
EP  - 109
DB  - TRDizin
UR  - http://search/yayin/detay/496989
ER  -