TY  - JOUR
TI  - Melatonin Enhances the Chemosensitivity of Pancreatic Carcinoma Cells (PANC-1) to Cisplatin and Cetuximab through modulation of p21, p27, p53, p57, MDM2 and KRAS Genes
AB  - We investigated the effects of melatonin, cetuximab and cisplatin treatments alone or in combination on PANC-1 cells from a human pancreatic carcinoma of ductal cell origin through cell viability and gene expressions. The cells were left for 48 h incubation after applying chemicals on the PANC-1 cells. The metabolic effects of the substances on cell viability at the end of incubation were measured by MTT assay. The gene expressions of p21, p27, p53, p57, MDM2 and KRAS were determined by RT-PCR. The use of melatonin combined with cisplatin or cetuximab increased p21 and p57 genes and decreased KRAS gene. Furthermore, melatonin combined with cetuximab increased p27 gene expression and decreased the cell viability compared to cetuximab alone. The cell viability was the lowest in cisplatin and cisplatin plus melatonin and/or cetuximab groups. The p53 were highest in the cisplatin groups while cisplatin plus melatonin decreased the p53 gene and its autoregulator MDM2 gene compared to cisplatin alone. In conclusion, melatonin in combinations with cisplatin and cetuximab enhances the tumor suppressor genes p21, p27 and p57 along with a modulation of the oncogenic gene KRAS suggesting the potential of melatonin as a therapeutic approach in combination therapy of pancreatic ductal adenocarcinoma.
AU  - GÜR, Cihan
AU  - ÖZKANLAR, Seçkin
DO  - 10.46810/tdfd.998059
PY  - 2021
JO  - Türk Doğa ve Fen Dergisi
VL  - 10
IS  - 2
SN  - 2149-6366
SP  - 275
EP  - 282
DB  - TRDizin
UR  - http://search/yayin/detay/502514
ER  -