TY  - JOUR
TI  - Effects of shoot tip size on in vitro regeneration and virus elimination of grapevine cv. Superior Seedless
AB  - In this study, we investigate the relation between the explant size and the success of virus elimination in ‘Superior Seedless’ grape variety. Plants were tested by prior to thermotherapy and in vitro culture by using Double Antibody Sandwich-Enzyme-Linked Immunosorbent Assay (DAS-ELISA) and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and verified as infected by different viruses (Grapevine leafroll associated virus -1+3, Grapevine leafroll associated virus -4 Strains, Grapevine rupestris stem pitting associated virus). Different size of shoot tip explants (0.1-0.5-1-5-10 mm) were excised and cultured on C 2 D medium supplemented with 1 mgl -1 BA. All of the explants bigger than 0.1 mm 100% regenerated while 70% of the 0.1 mm explants showed complete regeneration and turned into a whole plant. Plants regenerated from 10 mm shoot tip explants have all three viruses (GLRaV-1+3, GLRaV-4 strains and GRSPaV) as founded in mother plant. Plants regenerated from 5 mm explants have two of them (GLRaV-1+3, GLRaV-4 strains) and plants regenerated from 1 mm explants have one (GLRaV-1+3). Plants regenerated from 0.1 mm explants were found completely virus-free.
AU  - ÖNDER, Serkan
AU  - SAYGAÇ, Simin
DO  - 10.16955/bitkorb.877123
PY  - 2021
JO  - Bitki Koruma Bülteni
VL  - 61
IS  - 3
SN  - 0406-3597
SP  - 5
EP  - 9
DB  - TRDizin
UR  - http://search/yayin/detay/504865
ER  -