Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum
Yıl: 2021 Cilt: 26 Sayı: 4 Sayfa Aralığı: 620 - 627 Metin Dili: İngilizce DOI: 10.5578/flora.20219650 İndeks Tarihi: 17-05-2022
Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum
Öz: Introduction: Many studies have shown the advantages of monitoring wastewater in the evaluation of microbiological pathogens cir culating in the community. It was aimed to detect of SARS-CoV-2 RNA with a simple concentration method in wastewater in this study. Materials and Methods: In our study, 7 wastewater samples were investigated, which were collected from the Urban WastewaterTreatment Plant (WWTP) of Çorum, between October to November 2020. Sorbent bags were left in water for 24 hours. Then theywere used to trap and concentrate the virus. Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA detected by usingreverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays.Results: As a result, 3 of the 7 samples taken were positive for N and ORF1ab target gene regions.Conclusion: This is the first study reporting the detection of SARS-CoV-2 RNA in wastewater with different concentration and capturemethod.
Anahtar Kelime: Türkiye’de Atık Suda SARS-CoV-2 RNA’nın Basit bir Konsantrasyon Yöntemiyle TespitEdilmesi: Çorum’da Pilot Çalışma
Öz: Giriş: Toplumda dolaşan mikrobiyolojik patojenlerin izlenmesinde atık suyu izlemenin avantajları birçok çalışmayla gösterilmiştir. Bizdeçalışmamızda basit bir konsantrasyon metoduyla atık suda SARS-CoV-2 RNA tespit etmeyi amaçladık. Materyal ve Metod: Çalışmamızda Ekim-Kasım 2020 tarihleri arasında Çorum Kentsel Atık Su Arıtma Tesisi’nden toplanan 7 atık sunumunesi incelendi. Sorbent torbalar 24 saat suda bırakıldı. Torbalar virüsü yakalamak ve konsantre etmek için kullanıldı. SARS CoV-2RNA, ters transkripsiyon-kantitatif polimeraz zincir reaksiyonu (RT-qPCR) testleri kullanılarak saptandı. Bulgular: Sonuç olarak, alınan 7 numuneden 3’ü N ve ORF1ab hedef gen bölgeleri için pozitifti. Sonuç: Çalışmamız, farklı konsantrasyon ve yakalama yöntemi ile atık sularda SARS-CoV-2 RNA tespitini bildiren ilk çalışmadır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA | ŞENSÖZ S, Karasartova D, Savci U, GÜRESER A, ARSLAN AKVERAN G, Aktı M, Gürel B, Kocaman Ç, ACAR İ, Koşar N, Xiao L, ÖZKAN A (2021). Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum. , 620 - 627. 10.5578/flora.20219650 |
Chicago | ŞENSÖZ Sabiha,Karasartova Djursun,Savci Unsal,GÜRESER Ayşe Semra,ARSLAN AKVERAN GÖNÜL,Aktı Merve,Gürel Büşra,Kocaman Çağdaş,ACAR İsmail Aytaç,Koşar Nezahat,Xiao Lihua,ÖZKAN Ayşegül Taylan Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum. (2021): 620 - 627. 10.5578/flora.20219650 |
MLA | ŞENSÖZ Sabiha,Karasartova Djursun,Savci Unsal,GÜRESER Ayşe Semra,ARSLAN AKVERAN GÖNÜL,Aktı Merve,Gürel Büşra,Kocaman Çağdaş,ACAR İsmail Aytaç,Koşar Nezahat,Xiao Lihua,ÖZKAN Ayşegül Taylan Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum. , 2021, ss.620 - 627. 10.5578/flora.20219650 |
AMA | ŞENSÖZ S,Karasartova D,Savci U,GÜRESER A,ARSLAN AKVERAN G,Aktı M,Gürel B,Kocaman Ç,ACAR İ,Koşar N,Xiao L,ÖZKAN A Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum. . 2021; 620 - 627. 10.5578/flora.20219650 |
Vancouver | ŞENSÖZ S,Karasartova D,Savci U,GÜRESER A,ARSLAN AKVERAN G,Aktı M,Gürel B,Kocaman Ç,ACAR İ,Koşar N,Xiao L,ÖZKAN A Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum. . 2021; 620 - 627. 10.5578/flora.20219650 |
IEEE | ŞENSÖZ S,Karasartova D,Savci U,GÜRESER A,ARSLAN AKVERAN G,Aktı M,Gürel B,Kocaman Ç,ACAR İ,Koşar N,Xiao L,ÖZKAN A "Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum." , ss.620 - 627, 2021. 10.5578/flora.20219650 |
ISNAD | ŞENSÖZ, Sabiha vd. "Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum". (2021), 620-627. https://doi.org/10.5578/flora.20219650 |
APA | ŞENSÖZ S, Karasartova D, Savci U, GÜRESER A, ARSLAN AKVERAN G, Aktı M, Gürel B, Kocaman Ç, ACAR İ, Koşar N, Xiao L, ÖZKAN A (2021). Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum. Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi, 26(4), 620 - 627. 10.5578/flora.20219650 |
Chicago | ŞENSÖZ Sabiha,Karasartova Djursun,Savci Unsal,GÜRESER Ayşe Semra,ARSLAN AKVERAN GÖNÜL,Aktı Merve,Gürel Büşra,Kocaman Çağdaş,ACAR İsmail Aytaç,Koşar Nezahat,Xiao Lihua,ÖZKAN Ayşegül Taylan Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum. Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi 26, no.4 (2021): 620 - 627. 10.5578/flora.20219650 |
MLA | ŞENSÖZ Sabiha,Karasartova Djursun,Savci Unsal,GÜRESER Ayşe Semra,ARSLAN AKVERAN GÖNÜL,Aktı Merve,Gürel Büşra,Kocaman Çağdaş,ACAR İsmail Aytaç,Koşar Nezahat,Xiao Lihua,ÖZKAN Ayşegül Taylan Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum. Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi, vol.26, no.4, 2021, ss.620 - 627. 10.5578/flora.20219650 |
AMA | ŞENSÖZ S,Karasartova D,Savci U,GÜRESER A,ARSLAN AKVERAN G,Aktı M,Gürel B,Kocaman Ç,ACAR İ,Koşar N,Xiao L,ÖZKAN A Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum. Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi. 2021; 26(4): 620 - 627. 10.5578/flora.20219650 |
Vancouver | ŞENSÖZ S,Karasartova D,Savci U,GÜRESER A,ARSLAN AKVERAN G,Aktı M,Gürel B,Kocaman Ç,ACAR İ,Koşar N,Xiao L,ÖZKAN A Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum. Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi. 2021; 26(4): 620 - 627. 10.5578/flora.20219650 |
IEEE | ŞENSÖZ S,Karasartova D,Savci U,GÜRESER A,ARSLAN AKVERAN G,Aktı M,Gürel B,Kocaman Ç,ACAR İ,Koşar N,Xiao L,ÖZKAN A "Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum." Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi, 26, ss.620 - 627, 2021. 10.5578/flora.20219650 |
ISNAD | ŞENSÖZ, Sabiha vd. "Detection of SARS-CoV-2 RNA with a SimpleConcentration Method in Wastewater in Turkey:A Pilot Study in Çorum". Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi 26/4 (2021), 620-627. https://doi.org/10.5578/flora.20219650 |