The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model

Ozer, ilter; Yıldırım, Emine; Ozer, Hilal; YILDIRIM, Timur; Koyuncu, Adil

doi:10.14744/tjtes.2020.58901

The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model

Emine YILDIRIM, (Department of General Surgery, University of Health Sciences, Gaziosmanpaşa Training and Research Hospital, İstanbul-Turkey)

Hilal ÖZER, (Department of General Surgery, University of Health Sciences Turkey, Ankara Training and Research Hospital, Ankara-Turkey)

İlter ÖZER, (Department of General Surgery, Eskişehir Osmangazi University Faculty of Medicine, Eskişehir-Turkey)

Adil KOYUNCU, (Department of General Surgery, University of Health Sciences, Haseki Training and Research Hospital, İstanbul-Turkey)

Timur YILDIRIM (Department of Orthopedics and Traumatology, University of Health Sciences, Baltalimani Bone Diseases Training and Research Center, İstanbul-Turkey)

Ulusal Travma ve Acil Cerrahi Dergisi

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Yıl: 2021 Cilt: 27 Sayı: 4 Sayfa Aralığı: 389 - 394 Metin Dili: İngilizce DOI: 10.14744/tjtes.2020.58901 İndeks Tarihi: 18-05-2022

The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model

Öz:

BACKGROUND: The free oxygen radicals formed with reperfusion following intestinal ischaemia are extremely toxic for the cells. Glutathione peroxidase, an important enzyme that prevents the formation of reactive oxygen species, requires selenium as a co-factor. This study aims to demonstrate the effects of selenium administration on reducing ischaemia-reperfusion damage. METHODS: In this study, 28 male Wistar rats were separated into four groups. To Groups 3 and 4, sodium selenite at the dose of 10 µg/kg/day was administered intraperitoneally for five days. In Groups 1 and 3, laparotomy was applied, and in Groups 2 and 4, following laparotomy, ischaemia was created by clamping the superior mesenteric artery for 45 mins, then reperfusion was provided for 90 mins. Blood, liver and ileum samples were taken from all the animals for examination of malondialdehyde. For examination of bacterial translocation, liver, spleen and mesenteric lymph node tissue samples were taken. A sample taken from the ileum was examined histopathologically. RESULTS: There was determined to be significantly more bacterial translocation in the mesenteric lymph nodes of the ischaemia-reperfusion group (p<0.05). In the histopathological evaluation, the score in the ischaemia-reperfusion group was significantly higher than the scores in the other groups (p<0.05). Elevated serum, liver and ileum malondialdehyde levels in the ischaemia-reperfusion group were significantly higher than those in the other groups (p<0.05). CONCLUSION: Selenium was seen to have decreased serum and tissue malondialdehyde levels and increased the histopathological damage developing in the intestines with ischaemia-reperfusion and thereby increased bacterial translocation.

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APA	Yıldırım E, Ozer H, Ozer i, Koyuncu A, YILDIRIM T (2021). The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model. , 389 - 394. 10.14744/tjtes.2020.58901
Chicago	Yıldırım Emine,Ozer Hilal,Ozer ilter,Koyuncu Adil,YILDIRIM Timur The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model. (2021): 389 - 394. 10.14744/tjtes.2020.58901
MLA	Yıldırım Emine,Ozer Hilal,Ozer ilter,Koyuncu Adil,YILDIRIM Timur The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model. , 2021, ss.389 - 394. 10.14744/tjtes.2020.58901
AMA	Yıldırım E,Ozer H,Ozer i,Koyuncu A,YILDIRIM T The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model. . 2021; 389 - 394. 10.14744/tjtes.2020.58901
Vancouver	Yıldırım E,Ozer H,Ozer i,Koyuncu A,YILDIRIM T The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model. . 2021; 389 - 394. 10.14744/tjtes.2020.58901
IEEE	Yıldırım E,Ozer H,Ozer i,Koyuncu A,YILDIRIM T "The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model." , ss.389 - 394, 2021. 10.14744/tjtes.2020.58901
ISNAD	Yıldırım, Emine vd. "The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model". (2021), 389-394. https://doi.org/10.14744/tjtes.2020.58901

APA	Yıldırım E, Ozer H, Ozer i, Koyuncu A, YILDIRIM T (2021). The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model. Ulusal Travma ve Acil Cerrahi Dergisi, 27(4), 389 - 394. 10.14744/tjtes.2020.58901
Chicago	Yıldırım Emine,Ozer Hilal,Ozer ilter,Koyuncu Adil,YILDIRIM Timur The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model. Ulusal Travma ve Acil Cerrahi Dergisi 27, no.4 (2021): 389 - 394. 10.14744/tjtes.2020.58901
MLA	Yıldırım Emine,Ozer Hilal,Ozer ilter,Koyuncu Adil,YILDIRIM Timur The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model. Ulusal Travma ve Acil Cerrahi Dergisi, vol.27, no.4, 2021, ss.389 - 394. 10.14744/tjtes.2020.58901
AMA	Yıldırım E,Ozer H,Ozer i,Koyuncu A,YILDIRIM T The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model. Ulusal Travma ve Acil Cerrahi Dergisi. 2021; 27(4): 389 - 394. 10.14744/tjtes.2020.58901
Vancouver	Yıldırım E,Ozer H,Ozer i,Koyuncu A,YILDIRIM T The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model. Ulusal Travma ve Acil Cerrahi Dergisi. 2021; 27(4): 389 - 394. 10.14744/tjtes.2020.58901
IEEE	Yıldırım E,Ozer H,Ozer i,Koyuncu A,YILDIRIM T "The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model." Ulusal Travma ve Acil Cerrahi Dergisi, 27, ss.389 - 394, 2021. 10.14744/tjtes.2020.58901
ISNAD	Yıldırım, Emine vd. "The effects on bacterial translocation and tissue damage of selenium treatment in an experimental intestinal ischaemia-reperfusion model". Ulusal Travma ve Acil Cerrahi Dergisi 27/4 (2021), 389-394. https://doi.org/10.14744/tjtes.2020.58901