Yıl: 2022 Cilt: 9 Sayı: 1 Sayfa Aralığı: 29 - 36 Metin Dili: İngilizce DOI: 10.18596/jotcsa.952065 İndeks Tarihi: 29-07-2022

Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity

Öz:
Laccase is one of the enzymes that catalyze the oxidation of phenolic and non-phenolic substrates and show encouraging potential as a biocatalyst in the synthesis of bioactive compounds. It is known that phenolic acids have an antioxidant effect. Bovine serum albumin (BSA) shows gelling activity, and nutraceutical binding ability but it does not show antioxidant activity. In this study, BSA which has no antioxidant activity using laccase, started to show antioxidant activity with gallic acid (GA) conjugation. The synthesized conjugates were analyzed by polyacrylamide gel electrophoresis (PAGE), ultraviolet–visible spectrophotometry (UV-Vis), and Fourier-transform infrared spectroscopy (FTIR). Radical scavenging capacity for antioxidant activity was measured. GA-functionalized-BSA displayed greatly improved 2,2'-azinobis-(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2- picryl-hydrazyl (DPPH) radical scavenging capacities, compared with the untreated BSA. Protein–flavonoid conjugates can improve the natural properties of proteins, being promising products to be used in medical, food and polymer fields where antioxidant ability is an essential feature.
Anahtar Kelime: bsa Laccase bioconjugate gallic acid antioxidant activity

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Bat Özmatara M, Ertan F (2022). Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity. , 29 - 36. 10.18596/jotcsa.952065
Chicago Bat Özmatara Merve,Ertan Fatma Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity. (2022): 29 - 36. 10.18596/jotcsa.952065
MLA Bat Özmatara Merve,Ertan Fatma Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity. , 2022, ss.29 - 36. 10.18596/jotcsa.952065
AMA Bat Özmatara M,Ertan F Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity. . 2022; 29 - 36. 10.18596/jotcsa.952065
Vancouver Bat Özmatara M,Ertan F Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity. . 2022; 29 - 36. 10.18596/jotcsa.952065
IEEE Bat Özmatara M,Ertan F "Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity." , ss.29 - 36, 2022. 10.18596/jotcsa.952065
ISNAD Bat Özmatara, Merve - Ertan, Fatma. "Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity". (2022), 29-36. https://doi.org/10.18596/jotcsa.952065
APA Bat Özmatara M, Ertan F (2022). Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity. Journal of the Turkish Chemical Society, Section A: Chemistry, 9(1), 29 - 36. 10.18596/jotcsa.952065
Chicago Bat Özmatara Merve,Ertan Fatma Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity. Journal of the Turkish Chemical Society, Section A: Chemistry 9, no.1 (2022): 29 - 36. 10.18596/jotcsa.952065
MLA Bat Özmatara Merve,Ertan Fatma Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity. Journal of the Turkish Chemical Society, Section A: Chemistry, vol.9, no.1, 2022, ss.29 - 36. 10.18596/jotcsa.952065
AMA Bat Özmatara M,Ertan F Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity. Journal of the Turkish Chemical Society, Section A: Chemistry. 2022; 9(1): 29 - 36. 10.18596/jotcsa.952065
Vancouver Bat Özmatara M,Ertan F Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity. Journal of the Turkish Chemical Society, Section A: Chemistry. 2022; 9(1): 29 - 36. 10.18596/jotcsa.952065
IEEE Bat Özmatara M,Ertan F "Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity." Journal of the Turkish Chemical Society, Section A: Chemistry, 9, ss.29 - 36, 2022. 10.18596/jotcsa.952065
ISNAD Bat Özmatara, Merve - Ertan, Fatma. "Laccase-catalyzed conjugation of BSA mediated by gallic acid: Preparation, characterization, and antioxidant activity". Journal of the Turkish Chemical Society, Section A: Chemistry 9/1 (2022), 29-36. https://doi.org/10.18596/jotcsa.952065