Effects of Pycnogenol and Its Combinations with
Cisplatin on Hepatocellular Carcinoma Cell Viability

BECIT KIZILKAYA, Merve; BAŞARAN, NURŞEN; Aydin Dilsiz, Sevtap

Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability

Merve BECİT, (Atatürk Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, Erzurum, Türkiye)

Sevtap AYDIN, (Hacettepe Üniversitesi, Eczacılık Fakültesi, Farmasötik Toksikoloji Anabilim Dalı, Ankara, Türkiye)

Nurşen BAŞARAN (Hacettepe Üniversitesi, Eczacılık Fakültesi, Farmasötik Toksikoloji Anabilim Dalı, Ankara, Türkiye)

FABAD Journal of Pharmaceutical Sciences

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Yıl: 2021 Cilt: 46 Sayı: 1 Sayfa Aralığı: 13 - 22 Metin Dili: İngilizce İndeks Tarihi: 25-05-2022

Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability

Öz:

Many challenges of hepatocellular carcinoma treatment, such as side effects and drug resistance, still remain. Therefore, new improvements with high pharmaceutical function and low toxicity are needed. Recently, the efficacy of the combination therapy of antineoplastic drugs with natural products like pycnogenol has garnered attention. Pycnogenol® is a standardized extract from the bark of Pinus pinaster and consists of phenolic compounds. Pycnogenol is considered complementary in the treatments of some cancer besides its good tolerability and high safety. This study aims to reveal the synergistic effects of pycnogenol combination with cisplatin and its relation to human hepatocellular carcinoma (HepG2) cell viability. Effects of single and combined treatment on cell viability were evaluated in Chinese hamster lung fibroblasts (V79) and HepG2 cells using MTT assay. In HepG2 cells, this combined treatment showed a more cytotoxic effect than singledose groups. Pycnogenol increased the cytotoxicity of cisplatin at 500 μM for 24 h; at 250-500 μM for 48 h in V79 cells; and also, at 125-500 μM for 24 h; at 62.5-500 μM for 48 h in HepG2 cells (p<0.05). Our study is the first study to show that pycnogenol treatment with cisplatin has been combined in the HepG2 cell line. As a result, pycnogenol induced cisplatin cytotoxicity via combined treatment on HepG2 cells and exhibited a synergistic effect with cisplatin. In conclusion, pycnogenol may play a role in the chemotherapy of hepatocellular carcinoma; however, further studies are required to confirm their interactions with cisplatin.

Anahtar Kelime:

Piknogenol ve Sisplatin ile Kombinasyonlarının Hepatoselüler Karsinom Hücre Canlılığı Üzerine Etkileri

Öz:

Hepatoselüler karsinom tedavisinin yan etkiler ve ilaç direnci gibi birçok zorluğu devam etmektedir. Bu nedenle, farmasötik fonksiyonu yükseltecek ve toksisiteyi düşürecek yeni iyileştirmelere ihtiyaç vardır. Son zamanlarda, antineoplastik ilaçların piknogenol gibi doğal ürünlerle kombinasyonel tedavisinin etkinliği dikkat çekmektedir. Pycnogenol®, Pinus pinaster kabuğundan standartlaştırılmış bir ekstrakt olup fenolik bileşiklerden oluşur. Pycnogenolün, iyi tolere edilebilirliği ve yüksek güvenliği yanı sıra bazı kanserlerin tedavisinde de tamamlayıcı olduğu düşünülmektedir. Bu çalışmanın amacı, sisplatin ile piknogenol kombinasyonunun sinerjik etkilerini, bu etkilerin insan hepatosellüler karsinom (HepG2) hücre canlılığı ile nasıl bir ilişki olduğunu ortaya çıkarmaktır. Tek başlarına ve kombine olarak tedavinin hücre canlılığı üzerindeki etkileri, Çin hamsteri akciğer fibroblastlarında (V79) ve HepG2 hücrelerinde MTT kullanılarak değerlendirildi. HepG2 hücrelerindeki bu kombinasyon tedavi, tek doz gruplarından daha fazla sitotoksik etki gösterdi. Piknogenol, sisplatinin sitotoksisitesini V79 hücrelerinde 24 saatlik inkübasyonda 500 μM, 48 saatlik 250-500 μM konsantrasyonda ve ayrıca HepG2 hücrelerinde 24 saat için 125-500 μM, 48 saat için 62.5-500 μM’de anlamlı artırdı (p <0.05). Çalışmamız HepG2 hücre hattında piknogenolün sisplatin ile tedavisinin kombine edildiğini gösteren ilk çalışmadır. Sonuç olarak, Pycnogenol HepG2 hücreleri üzerinde kombine tedavi ile sisplatin sitotoksisitesini indükledi ve sisplatin ile sinerjistik bir etki gösterdi. Sonuç olarak, piknogenol, hepatosellüler karsinomun kemoterapisinde rol oynayabilir; bununla birlikte, sisplatin ile etkileşimlerini doğrulamak için daha ileri çalışmalara ihtiyaç vardır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BECIT KIZILKAYA M, Aydin Dilsiz S, BAŞARAN N (2021). Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability. , 13 - 22.
Chicago	BECIT KIZILKAYA Merve,Aydin Dilsiz Sevtap,BAŞARAN NURŞEN Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability. (2021): 13 - 22.
MLA	BECIT KIZILKAYA Merve,Aydin Dilsiz Sevtap,BAŞARAN NURŞEN Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability. , 2021, ss.13 - 22.
AMA	BECIT KIZILKAYA M,Aydin Dilsiz S,BAŞARAN N Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability. . 2021; 13 - 22.
Vancouver	BECIT KIZILKAYA M,Aydin Dilsiz S,BAŞARAN N Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability. . 2021; 13 - 22.
IEEE	BECIT KIZILKAYA M,Aydin Dilsiz S,BAŞARAN N "Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability." , ss.13 - 22, 2021.
ISNAD	BECIT KIZILKAYA, Merve vd. "Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability". (2021), 13-22.

APA	BECIT KIZILKAYA M, Aydin Dilsiz S, BAŞARAN N (2021). Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability. FABAD Journal of Pharmaceutical Sciences, 46(1), 13 - 22.
Chicago	BECIT KIZILKAYA Merve,Aydin Dilsiz Sevtap,BAŞARAN NURŞEN Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability. FABAD Journal of Pharmaceutical Sciences 46, no.1 (2021): 13 - 22.
MLA	BECIT KIZILKAYA Merve,Aydin Dilsiz Sevtap,BAŞARAN NURŞEN Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability. FABAD Journal of Pharmaceutical Sciences, vol.46, no.1, 2021, ss.13 - 22.
AMA	BECIT KIZILKAYA M,Aydin Dilsiz S,BAŞARAN N Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability. FABAD Journal of Pharmaceutical Sciences. 2021; 46(1): 13 - 22.
Vancouver	BECIT KIZILKAYA M,Aydin Dilsiz S,BAŞARAN N Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability. FABAD Journal of Pharmaceutical Sciences. 2021; 46(1): 13 - 22.
IEEE	BECIT KIZILKAYA M,Aydin Dilsiz S,BAŞARAN N "Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability." FABAD Journal of Pharmaceutical Sciences, 46, ss.13 - 22, 2021.
ISNAD	BECIT KIZILKAYA, Merve vd. "Effects of Pycnogenol and Its Combinations with Cisplatin on Hepatocellular Carcinoma Cell Viability". FABAD Journal of Pharmaceutical Sciences 46/1 (2021), 13-22.