TY  - JOUR
TI  - Molecular detection of E. faecalis in oral samples of a
population associated with secondary endodontic
infection
AB  - Aim: The objective of this study was to evaluate the prevalence of
Enterococcus faecalis in samples of oral rinse and tongue dorsum of
endodontic patients with secondary/persistent infections (EPSI) using the
PCR method.
Methodology: Oral rinse samples (ORS) and tongue swab samples (TSS)
of 22 patients (EPSI group) and 32 healthy individuals (control group) were
collected. DNA isolation from the TSS and ORS samples was performed
using the modified classical phenol-chloroform and chloroform method. To
detect E. faecalis strains directly from the TSS and ORS samples, the 310
base pair (bp) segment of the 16S rDNA of the E. faecalis genome was
amplified by PCR using specific primers. The prevalence of E. faecalis was
compared between healthy and sick individuals using the Chi-square test,
significance was set at p<0.05.
Results: In the ORS samples, there was a significant difference between
the healthy individuals (n = 11, 34%) and the EPSI group (n = 15, 68%) in
terms of the presence of E. faecalis (p = 0.026). In the TSS, the presence
of E. faecalis was also investigated, and a significant difference was found
between healthy individuals (n = 3, 9%) and the EPSI group (n = 11, 50%) (p
= 0.001). In the EPSI group, no statistically significant difference was
present in the prevalence rate of E. faecalis between the samples of ORS
(68%) and TSS (50%) (p = 0.358).
Conclusion: The prevalence of E. faecalis was found to be statistically
significantly higher in multi-site oral samples of a population with
secondary endodontic infection than healthy individuals.
AU  - ASLAN, GÖNÜL
AU  - Kucuk, Mustafa Oner
AU  - Aslan, Tugrul
AU  - ERSOY, LEYLA
AU  - KESİM, Bertan
AU  - Tezcan Ülger, Seda
AU  - CUDAL, Hamza
DO  - 10.5577/intdentres.2021.vol11.no3.7
PY  - 2021
JO  - International Dental Research
VL  - 11
IS  - 3
SN  - 2146-1767
SP  - 180
EP  - 184
DB  - TRDizin
UR  - http://search/yayin/detay/509261
ER  -