LC-HRMS profiling of phytochemicals, antidiabetic,
anticholinergic and antioxidant activities of evaporated ethanol
extract of Astragalus brachycalyx Fischer

Gulcin, ilhami; Goren, Ahmet C; Alwasel, Saleh; kızıltaş, hatice; bingöl, zeynebe; Pınar, Süleyman Mesut

doi:10.25135/jcm.62.2107.2155

LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer

Hatice KIZILTAŞ, (Van Yüzüncü Yıl Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Van, Türkiye)

Zeynebe BİNGÖL, (Tokat Gaziosmanpaşa Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tokat, Türkiye. Atatürk Üniversitesi, Fen Fakültesi, Kimya Bölümü, Erzurum, Türkiye)

Ahmet C. GÖREN, (Gebze Teknik Üniversitesi,Temel Bilimler Fakültesi, Kimya Bölümü, Kocaeli, Türkiye. Bezmialem Vakıf Üniversitesi, İlaç Uygulama ve Araştırma Merkezi (İLMER), İstanbul, Türkiye)

Süleyman Mesut PINAR, (Yüzüncü Yıl Üniversitesi, Van Sağlık Yüksekokulu, Van, Türkiye)

Saleh H. ALWASEL, (King Saud University, College of Science, Department of Zoology, Riyadh, Saudi Arabia)

İlhami GÜLÇİN (Atatürk Üniversitesi, Fen Fakültesi, Kimya Bölümü, Erzurum, Türkiye)

Journal of Chemical Metrology

16 0

Yıl: 2021 Cilt: 15 Sayı: 2 Sayfa Aralığı: 135 - 151 Metin Dili: İngilizce DOI: 10.25135/jcm.62.2107.2155 İndeks Tarihi: 26-05-2022

LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer

Öz:

Astragalus is a perennial plant that has existed for about 2500-3000 years and consists of more than 250 taxonomic parts. Twenty species of Astragalus are endemic to Turkey, as well as the richest genus with 425 taxa. The roots of Astragalus species are used in folk medicine as hepatoprotective, antioxidant, antibacterial, antihypertensive, antidiabetic and diuretic. Also, it is used to treat diabetes mellitus, leukemia, nephritis and uterine cancer. It is known that in Anatolia, Astragalus roots are traditionally used against leukemia and wound healing. For the purpose, the measuring of antioxidant activity of evaporated ethanol extract of Astragalus brachycalyx FISCHER (EEAB), some bioanalytic methods including DPPH• and ABTS•+ scavenging effects, ferric ions (Fe3+) and cupric ions (Cu2+ ) reducing abilities, and metal (Fe2+) chelating activity were realized. α-Tocopherol, ascorbic acid, and BHT were used as the standard antioxidants. On the other hand, some phenolic compounds, which responsible for antioxidant activities of EEAB was determined by liquid chromatography-high resolution mass spectrometry (LC-HRMS). At the similar concentration, EEAB exhibited efficient antioxidant effects when compared to standard compounds. Additionally, EEAB showed IC50 values of 1.985 μg/mL toward acetylcholinesterase (AChE), 0.620 μg/mL on α-glycosidase and 0.306 μg/mL against α-amylase enzymes.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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[85] A. Labed, M. Ferhat, I. Labed-Zouad, E. Kaplaner, S. Zerizer, L. Voutquenne-Nazabadioko, A.A. Magid, Z. Semra, A. Kabouche, Z. Kabouche and M. Öztürk (2016). Compounds from the pods of Astragalus armatus with antioxidant, anticholinesterase, antibacterial and phagocytic activities, Pharm. Biol. 54(12), 3026-3032.
[86] J. Benites, D. Rios, A. Guerrero-Castilla, C. Enriquez, E. Zavala, R.O. Ybanez-Julca, I. Quispe-Diaz, R. Jara-Aguilar and P.B. Calderon (2021). Chemical composition and assessment of antimicrobial, antioxidant and antiproliferative activities of essential oil from Clinopodium sericeum, a peruvian medicinal plant, Rec. Nat. Prod. 15(3), 175-186.
[87] N. Oztaskın, Y. Çetinkaya, P. Taslimi, S. Göksu and İ. Gülçin (2015). Antioxidant and acetylcholinesterase inhibition properties of novel bromophenol derivatives, Bioorg. Chem. 60, 49-57.
[88] M.H. Sehitoglu, H. Han, P. Kalin, İ. Gülçin, A. Ozkan and H.Y. Aboul-Enein (2015). Pistachio (Pistacia vera L.) gum: A potent inhibitor of reactive oxygen species, J. Enzyme Inhib. Med. Chem. 30, 264-269.
[89] İ. Gülçin, M. Elmastaş and H.Y. Aboul-Enein (2012). Antioxidant activity of clove oil-A powerful antioxidant source, Arab. J. Chem. 5(4), 489-499.
[90] S. Langari and E.A. Salehi (2015). Evaluation of the antioxidant capacity of different extracts of Astragalus glaucacanthus, J. Appl. Environ. Biol. Sci. 4(11S), 14-17.
[91] Y. Çetinkaya, H. Göçer, A. Menzek and İ. Gülçin (2012). Synthesis and antioxidant properties of (3,4- dihydroxyphenyl)(2,3,4-trihydroxyphenyl)methanone and its derivatives, Arch. Pharm. 345, 323-334.
[92] İ. Gülçin, F. Topal, R. Çakmakçı, A.C. Gören, M. Bilsel and U. Erdoğan (2011). Pomological features, nutritional quality, polyphenol content analysis and antioxidant properties of domesticated and three wild ecotype forms of raspberries (Rubus idaeus L.), J. Food Sci. 76(4), C585-C593.
[93] İ. Gülçin (2011). Antioxidant activity of eugenol-a structure and activity relationship study, J. Med. Food, 14(9), 975-985.
[94] B. Butkut, A. Dagilyt, R. Benetis, A. Padarauskas, J. CeseviIien, V. Olšauskait and N. Lemedien (2018). Mineral and phytochemical profiles and antioxidant activity of herbal material from two temperate astragalus species, BioMed Res. Int. 2018, 1-11
[95] J. Asgarpanah, S.M. Motamed, A. Farzaneh, B. Ghanizadeh and S. Tomraee, (2011). Antioxidant activity and total phenolic and flavonoid content of Astragalus squarrosus Bunge, Afr. J. Biotechnol. 10(82), 19176-19180.
[96] A. Baghiani, N. Boussoualim, H. Trabsa, S. Aouachria and L. Arrar (2013). In vivo free radical scavenging, antihemolytic activity and antibacterial effects of Anchusa Azurea extracts, Int. J. Med. Med. Sci. 46(1), 1113-1118.
[97] H. Şerbetçi Tohma and İ. Gülçin (2010). Antioxidant and radical scavenging activity of aerial parts and roots of Turkish liquorice (Glycyrrhiza glabra L.), Int. J. Food Prop. 13(4), 657-671.
[98] İ. Gülçin, E. Kirecci, E. Akkemik, F. Topal and O. Hisar (2010). Antioxidant and antimicrobial activities of an aquatic plant: Duckweed (Lemna minor L.), Turk. J. Biol. 34(2), 175-188.
[99] İ. Gülçin, A.Z. Tel and E. Kirecci (2008). Antioxidant, antimicrobial, antifungal and antiradical activities of Cyclotrichium niveum (Boiss.) Manden and Scheng, Int. J. Food Prop. 11(2), 450-471.
[100] F.M. Abdel Bar (2021) Genus Melaleuca - A review on the phytochemistry and pharmacological activities of the non-volatile components, Rec. Nat. Prod. 15(4), 219-242.
[101] İ. Gülçin, R. Elias, A. Gepdiremen, L. Boyer and E. Köksal (2007). A comparative study on the antioxidant activity of fringe tree (Chionanthus virginicus L.) extracts, Afr. J. Biotechnol. 6(4), 410-418.
[102] İ. Gülçin, M. Elmastas and H.Y. Aboul-Enein (2007). Determination of antioxidant and radical scavenging activity of basil (Ocimum basilicum) assayed by different methodologies. Phytother. Res. 21, 354-361.
[103] A. Musa, K.F. El-Masrry, A.H. El-Ghorab, A. Farouk, H.M. Ali, M.A. Abdelgawad, I.A. Naguib and E.M. Mostafa (2021). Volatile constituents of Cistanche tubulosa and their antioxidant and antimicrobial potentials, Rec. Nat. Prod. 15(4), 301-311.
[104] M. Imran, A. Rauf, T.A. Izneid, M. Nadeem, M.A. Shariati, I.A. Khan, A. Imran, I.E. Orhan, M. Rizwan, M. Atif, T.A. Gondal and M.S. Mubarak (2019). Luteolin, a flavonoid, as an anticancer agent: a review, Biomed. Pharmacother. 112, 108612.
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APA	kızıltaş h, bingöl z, Goren A, Pınar S, Alwasel S, Gulcin i (2021). LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer . , 135 - 151. 10.25135/jcm.62.2107.2155
Chicago	kızıltaş hatice,bingöl zeynebe,Goren Ahmet C,Pınar Süleyman Mesut,Alwasel Saleh,Gulcin ilhami LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer . (2021): 135 - 151. 10.25135/jcm.62.2107.2155
MLA	kızıltaş hatice,bingöl zeynebe,Goren Ahmet C,Pınar Süleyman Mesut,Alwasel Saleh,Gulcin ilhami LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer . , 2021, ss.135 - 151. 10.25135/jcm.62.2107.2155
AMA	kızıltaş h,bingöl z,Goren A,Pınar S,Alwasel S,Gulcin i LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer . . 2021; 135 - 151. 10.25135/jcm.62.2107.2155
Vancouver	kızıltaş h,bingöl z,Goren A,Pınar S,Alwasel S,Gulcin i LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer . . 2021; 135 - 151. 10.25135/jcm.62.2107.2155
IEEE	kızıltaş h,bingöl z,Goren A,Pınar S,Alwasel S,Gulcin i "LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer ." , ss.135 - 151, 2021. 10.25135/jcm.62.2107.2155
ISNAD	kızıltaş, hatice vd. "LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer ". (2021), 135-151. https://doi.org/10.25135/jcm.62.2107.2155

APA	kızıltaş h, bingöl z, Goren A, Pınar S, Alwasel S, Gulcin i (2021). LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer . Journal of Chemical Metrology, 15(2), 135 - 151. 10.25135/jcm.62.2107.2155
Chicago	kızıltaş hatice,bingöl zeynebe,Goren Ahmet C,Pınar Süleyman Mesut,Alwasel Saleh,Gulcin ilhami LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer . Journal of Chemical Metrology 15, no.2 (2021): 135 - 151. 10.25135/jcm.62.2107.2155
MLA	kızıltaş hatice,bingöl zeynebe,Goren Ahmet C,Pınar Süleyman Mesut,Alwasel Saleh,Gulcin ilhami LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer . Journal of Chemical Metrology, vol.15, no.2, 2021, ss.135 - 151. 10.25135/jcm.62.2107.2155
AMA	kızıltaş h,bingöl z,Goren A,Pınar S,Alwasel S,Gulcin i LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer . Journal of Chemical Metrology. 2021; 15(2): 135 - 151. 10.25135/jcm.62.2107.2155
Vancouver	kızıltaş h,bingöl z,Goren A,Pınar S,Alwasel S,Gulcin i LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer . Journal of Chemical Metrology. 2021; 15(2): 135 - 151. 10.25135/jcm.62.2107.2155
IEEE	kızıltaş h,bingöl z,Goren A,Pınar S,Alwasel S,Gulcin i "LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer ." Journal of Chemical Metrology, 15, ss.135 - 151, 2021. 10.25135/jcm.62.2107.2155
ISNAD	kızıltaş, hatice vd. "LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer ". Journal of Chemical Metrology 15/2 (2021), 135-151. https://doi.org/10.25135/jcm.62.2107.2155