TY  - JOUR
TI  - LC-HRMS profiling of phytochemicals, antidiabetic,
anticholinergic and antioxidant activities of evaporated ethanol
extract of Astragalus brachycalyx Fischer
AB  - Astragalus is a perennial plant that has existed for about 2500-3000 years and consists of more than
250 taxonomic parts. Twenty species of Astragalus are endemic to Turkey, as well as the richest genus with 425
taxa. The roots of Astragalus species are used in folk medicine as hepatoprotective, antioxidant, antibacterial,
antihypertensive, antidiabetic and diuretic. Also, it is used to treat diabetes mellitus, leukemia, nephritis and uterine
cancer. It is known that in Anatolia, Astragalus roots are traditionally used against leukemia and wound healing.
For the purpose, the measuring of antioxidant activity of evaporated ethanol extract of Astragalus brachycalyx
FISCHER (EEAB), some bioanalytic methods including DPPH•
and ABTS•+ scavenging effects, ferric ions (Fe3+)
and cupric ions (Cu2+
) reducing abilities, and metal (Fe2+) chelating activity were realized. α-Tocopherol, ascorbic
acid, and BHT were used as the standard antioxidants. On the other hand, some phenolic compounds, which
responsible for antioxidant activities of EEAB was determined by liquid chromatography-high resolution mass
spectrometry (LC-HRMS). At the similar concentration, EEAB exhibited efficient antioxidant effects when
compared to standard compounds. Additionally, EEAB showed IC50 values of 1.985 μg/mL toward
acetylcholinesterase (AChE), 0.620 μg/mL on α-glycosidase and 0.306 μg/mL against α-amylase enzymes.
AU  - Gulcin, ilhami
AU  - Goren, Ahmet C
AU  - Alwasel, Saleh
AU  - kızıltaş, hatice
AU  - bingöl, zeynebe
AU  - Pınar, Süleyman Mesut
DO  - 10.25135/jcm.62.2107.2155
PY  - 2021
JO  - Journal of Chemical Metrology
VL  - 15
IS  - 2
SN  - 1307-6183
SP  - 135
EP  - 151
DB  - TRDizin
UR  - http://search/yayin/detay/509754
ER  -