TY  - JOUR
TI  - Cloning and Expression of Rat Brain Acetylcholinesterase Enzyme in Escherichia coli
AB  - In this study, the gene region of rat (Rattus norvegicus) brain acetylcholinesterase enzyme was amplified in PCR with designed forward and reverse primers and it was ligated into a pET-SUMO vector under suitable conditions. This recombinant vector was transformed to competent Escherichia coli cells and it was grown in liquid LB medium including kanamycin. Colony PCR was performed from growing colony and PCR products were checked with agarose gel electrophoresis. The correct colonies were grown in a liquid medium for plasmid isolation. After plasmid isolation, these recombinant constructs were used for whether the gene inserts properly with cross-PCR. After determining the accuracy of the plasmid, recombinant vectors were transferred into the E. coli BL21 (DE3) cells to perform protein production. Cells were grown in IPTG induced larger media for hours. Enzyme activity and SDS-PAGE analysis were performed from homogenate for each treatment samples.
AU  - Karakus, Ahmet
AU  - ERDOĞAN, Orhan
AU  - ceylan, hamid
DO  - 10.21597/jist.962268
PY  - 2022
JO  - Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi
VL  - 12
IS  - 1
SN  - 2146-0574
SP  - 287
EP  - 296
DB  - TRDizin
UR  - http://search/yayin/detay/509876
ER  -