TY  - JOUR
TI  - Effect of Rutin on Gentamicin-Induced Ototoxicity in Rats: A Biochemical
and Histopathological Examination
AB  - Objective: Gentamicin is a broad-spectrum aminoglycoside antibiotic administered
parenterally for moderate to severe gram-negative infections. Ototoxicity is an important
side effect that limits gentamicin use. The aim of this study is to investigate
the effect of rutin on gentamicin-induced ototoxicity in rats biochemically and histopathologically.
Methods: Distilled water was administered by oral gavage to healthy controls (HG)
and cobalt administered group (GC). 50 mg/kg rutin was administered by oral gavage
to rutin + gentamicin (RGG) group. After one hour, 100 mg/kg gentamicin was injected
intraperitoneally (i.p) to the RGG and GC animal groups. This procedure was repeated
once a day for 14 days.
Results: Malondialdehyde (MDA), nuclear factor-κB(NF-κB), tumor necrosis factor
alpha (TNF-α) and interleukin-1 beta(IL-1β) levels in the cochlear nerve tissue of gentamicin-
treated animals were significantly higher compared to healthy controls and
rutin + gentamicin treated rats. On the other hand, the amount of total Glutathione
(tGSH) was significantly lower compared to the control and rutin group. Histopathological
examination revealed degenerated myelinated nerve fibers in the gentamicin
group and Schwann cell nuclei were generally not seen. There was a high accumulation
of collagen fiber in the tissue and dilated blood capillaries. In the rutin group, myelinated
nerve fibers mostly exhibited normal morphology, Schwann cell nuclei were
evident and the vessels were normal.
Conclusion: Our experimental results suggest that rutin may be effective in the treatment
of gentamicin-associated ototoxicity.
AU  - ÇOBAN, Abdulkadir
AU  - BAYRAM, Rana
AU  - salcan, ismail
AU  - Suleyman, Halis
AU  - YAZICI, Gülce Naz
AU  - Dilber, Muhammet
DO  - 10.5152/entupdates.2021.887158
PY  - 2021
JO  - ENT Updates
VL  - 11
IS  - 1
SN  - 2149-7109
SP  - 8
EP  - 13
DB  - TRDizin
UR  - http://search/yayin/detay/510329
ER  -