TY  - JOUR
TI  - POLY I:C-INDUCED TLR3 ACTIVATION ON OXIDATIVE STRESS LEVELS IN PC-3
(HORMONE-INSENSITIVE) AND LNCAP (HORMONE-SENSITIVE) AS PROSTATE CANCER
CELLS
AB  - Objective: We aimed to determine the role of Poly I:C-induced TLR3 activation on oxidative stress in two different prostate cancer cells [PC-3
(hormone insensitive) and LNCaP (hormone-sensitive)] for the first time. For this purpose, lipid peroxidation (MDA), hydrogen peroxide (H 2O2)
and proline amounts and superoxide dismutase (SOD) enzyme activity were examined.
Methods: The optimal concentration and time required for receptor stimulation with Poly I:C cells were determined by WST-1 analysis.
Spectrophotometric methods determined biochemical parameters.
Results: The less cytotoxic concentration of 5 μM of Poly I:C on PC-3 and LNCaP cells was determined. A significant increase was observed in
LNCaP cells in SOD activities after 6 and 24 hours. A significant increase in PC-3 and LNCaP cells' MDA levels was determined over 6 hours,
while a significant decrease was observed in Poly I:C LNCaP after 24 hours. A significant increase in H 2O2 concentration was detected in LNCaP
cells, but a significant decrease was observed in PC-3 after 6 and 24 hours. The proline level showed a significant increase in LNCaP over 24
hours but not in the proline level in PC-3 cells after 6 and 24 hours.
Conclusion: The MDA, H 2O2 and SOD activity levels were found to be significantly higher in hormone-sensitive LNCaP cells, while no
significant changes were found in PC-3 cells treated with Poly I:C. Results were significantly different at the level of p<0.05 and p<0.001.
AU  - Deveci Özkan, Asuman
AU  - Yuksel, Burcu
DO  - 10.30934/kusbed.915511
PY  - 2022
JO  - Kocaeli Üniversitesi Sağlık Bilimleri Dergisi
VL  - 8
IS  - 1
SN  - 2149-8571
SP  - 18
EP  - 24
DB  - TRDizin
UR  - http://search/yayin/detay/511141
ER  -