TY  - JOUR
TI  - Fragmentation Analysis of Plasma DNA Reveals Its
Prognostic Value in Gastric Cancer
AB  - Background: Gastric cancer (GC) is a common cause of cancer-related deaths. The poor clinical outcome in GC patients is partially
associated with a lack of appropriate diagnostic and prognostic biomarkers. In the present study, we evaluated the diagnostic and
prognostic values of cell-free DNA (cfDNA) integrity and the concentration of circulating nucleosomes (cNUCs).
Methods: In the study, 40 GC patients and 55 GC-free individuals were enrolled. Cell-free DNA integrity was calculated as the ratio of
concentration of the longer ACTB (beta-actin) gene fragment to that of the shorter ACTB fragment, measured using quantitative PCR.
Circulating nucleosomes were measured by an ELISA-based approach.
Results: We found that cfDNA integrity is higher in GC patients than in the control subjects (relative median values 0.51 vs. 0.38, respectively,
P = .56) indicating prominent abundance of longer fragments in the patients. The patients with larger tumors (T3-4) had significantly
higher cfDNA integrity than those with T1-T2 tumors. We also found GC patients to have higher concentrations of cNUCs in their
plasma (relative median values 3.64 vs. 3.1). Importantly, the patients with high cfDNA integrity (i.e., lower fragmentation) had longer
overall survival rates at 3 years than those with lower cfDNA integrity (76.5% vs. 38.9%, P = .02).
Conclusion: Cell-free DNA fragmentation has a prognostic value. However, it has no diagnostic value in GC.
AU  - Ozgur, Ilker
AU  - Yoruker, Ebru Esin
AU  - Özgür, Emre
AU  - Keskin, Metin
AU  - Gezer, Ugur
DO  - 10.5152/tjg.2021.20832
PY  - 2021
JO  - Turkish Journal of Gastroenterology
VL  - 32
IS  - 9
SN  - 1300-4948
SP  - 720
EP  - 726
DB  - TRDizin
UR  - http://search/yayin/detay/512596
ER  -