Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn”
sequences

YILMAZ SANCAR, PELİN; MUNZUROĞLU, Ömer; Kurşat, Murat; Icen Taskin, Irmak

doi:10.46309/biodicon.2021.963174

Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences

Pelin YILMAZ SANCAR, (Fırat Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, 23119, Elazığ, Türkiye)

Irmak İÇEN TAŞKIN, (İnönü Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, 44280, Malatya, Türkiye)

Murat KURŞAT, (Bitlis Eren Üniversitesi, Fen-Edebiyat Fakültesi, Biyoloji Bölümü, 13000, Bitlis, Türkiye)

Ömer MUNZUROĞLU (Fırat Üniversitesi, Mühendislik Fakültesi, Biyomühendislik Bölümü, 23119, Elazığ, Türkiye)

Biological Diversity and Conservation

3 0

Yıl: 2021 Cilt: 14 Sayı: 3 Sayfa Aralığı: 431 - 441 Metin Dili: İngilizce DOI: 10.46309/biodicon.2021.963174 İndeks Tarihi: 02-06-2022

Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences

Öz:

Fritillaria baskilensis Behçet., which was first collected from Elazig - Baskil district in 1998 and introduced to the scientific world as a new species, is one of Turkey's endemic inverted tulip species. This study aims to determine the systematic relationships between Fritillaria baskilensis and other Fritillaria species using molecular techniques. Also; As a result of this study, we tried to determine for the first time in which subgenus the endemic species in question will take place. After the DNA isolation with the CTAB method, the trnT-trnL3' region of 5 samples was amplified by using trna and trnd primers, and a total of 27 Fritillaria species were analyzed together with 2 outgroups in the MEGA program, together with other sequence information obtained from NCBI. While our analyzes are consistent with the literature, the fact that Fritillaria baskilensis is in the same branch as members of the subgenus Fritillaria suggests that F. baskilensis is included in this subgenus. This study is very important as it is the first molecular-based study using sequence information from Fritillaria baskilensis populations, and the new “trn” gene region haplotypes of the mentioned species were determined by us for the first time and added to the GenBank database

Anahtar Kelime:

Endemik Fritillaria baskilensis Behçet (Liliaceae) için filogenetik analiz: cpDNA “trn” dizilerinden kanıtlarla

Öz:

İlk defa 1998 yılında Elazığ’ın Baskil ilçesinden toplanan ve bilim dünyasına yeni tür olarak kazandırılan Fritillaria baskilensis Behçet Türkiye’nin endemik ters lale türlerinden biridir. Bu çalışmanın amacı Fritillaria baskilensis’ in diğer Fritillaria türleri ile aralarındaki sistematik ilişkilerin moleküler teknikler kullanarak belirlenmesidir. Ayrıca; söz konusu endemik türün hangi altcinste yer alacağı da bu çalışmanın sonucu olarak ilk defa tarafımızca tespit edilmeye çalışılmıştır. CTAB metodu ile manuel olarak gerçekleştirilen DNA izolasyonu sonrası trna ve trnd primerleri kullanılarak 5 örneğe ait trnT-trnL3’ bölgesi çoğaltılmış, NCBI’ dan elde edilen diğer sekans bilgileri ile birlikte toplam 27 Fritillaria türü 2 dış grup ile birlikte MEGA programında analiz edilmiştir. Analizlerimiz literatürle uyumluluk gösterirken, F. baskilensis’ in Fritillaria altcinsi üyeleri ile aynı kolda yer alması F. baskilensis’in bu altcinste yer aldığı fikrini düşündürmektedir. Bu çalışma F. baskilensis populasyonlarına ait sekans bilgileri kullanılarak yapılmış ilk moleküler tabanlı çalışma olması açısından oldukça önemlidir ve sözkonusu türe ait yeni “trn” gen bölgesi haplotipleri ilk defa tarafımızca tespit edilerek GenBank veritabanına kazandırılmıştır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	YILMAZ SANCAR P, Icen Taskin I, Kurşat M, MUNZUROĞLU Ö (2021). Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences. , 431 - 441. 10.46309/biodicon.2021.963174
Chicago	YILMAZ SANCAR PELİN,Icen Taskin Irmak,Kurşat Murat,MUNZUROĞLU Ömer Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences. (2021): 431 - 441. 10.46309/biodicon.2021.963174
MLA	YILMAZ SANCAR PELİN,Icen Taskin Irmak,Kurşat Murat,MUNZUROĞLU Ömer Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences. , 2021, ss.431 - 441. 10.46309/biodicon.2021.963174
AMA	YILMAZ SANCAR P,Icen Taskin I,Kurşat M,MUNZUROĞLU Ö Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences. . 2021; 431 - 441. 10.46309/biodicon.2021.963174
Vancouver	YILMAZ SANCAR P,Icen Taskin I,Kurşat M,MUNZUROĞLU Ö Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences. . 2021; 431 - 441. 10.46309/biodicon.2021.963174
IEEE	YILMAZ SANCAR P,Icen Taskin I,Kurşat M,MUNZUROĞLU Ö "Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences." , ss.431 - 441, 2021. 10.46309/biodicon.2021.963174
ISNAD	YILMAZ SANCAR, PELİN vd. "Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences". (2021), 431-441. https://doi.org/10.46309/biodicon.2021.963174

APA	YILMAZ SANCAR P, Icen Taskin I, Kurşat M, MUNZUROĞLU Ö (2021). Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences. Biological Diversity and Conservation, 14(3), 431 - 441. 10.46309/biodicon.2021.963174
Chicago	YILMAZ SANCAR PELİN,Icen Taskin Irmak,Kurşat Murat,MUNZUROĞLU Ömer Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences. Biological Diversity and Conservation 14, no.3 (2021): 431 - 441. 10.46309/biodicon.2021.963174
MLA	YILMAZ SANCAR PELİN,Icen Taskin Irmak,Kurşat Murat,MUNZUROĞLU Ömer Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences. Biological Diversity and Conservation, vol.14, no.3, 2021, ss.431 - 441. 10.46309/biodicon.2021.963174
AMA	YILMAZ SANCAR P,Icen Taskin I,Kurşat M,MUNZUROĞLU Ö Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences. Biological Diversity and Conservation. 2021; 14(3): 431 - 441. 10.46309/biodicon.2021.963174
Vancouver	YILMAZ SANCAR P,Icen Taskin I,Kurşat M,MUNZUROĞLU Ö Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences. Biological Diversity and Conservation. 2021; 14(3): 431 - 441. 10.46309/biodicon.2021.963174
IEEE	YILMAZ SANCAR P,Icen Taskin I,Kurşat M,MUNZUROĞLU Ö "Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences." Biological Diversity and Conservation, 14, ss.431 - 441, 2021. 10.46309/biodicon.2021.963174
ISNAD	YILMAZ SANCAR, PELİN vd. "Phylogenetic analysis for endemic Fritillaria baskilensis Behçet (Liliaceae): Evidence from cpDNA “trn” sequences". Biological Diversity and Conservation 14/3 (2021), 431-441. https://doi.org/10.46309/biodicon.2021.963174