TY  - JOUR
TI  - Determination of lipase activities of actinomycetes isolates from soil
AB  - In this study 156 actinomycetes isolates which were isolated from different soil samples were used. Isolates 
were inoculated proper medium and incubated at 28°C for 7-10 days. In order to investigate the lipolytic activities of 
the isolates in the culture medium, the transparent zone formed by isolates in the prepared lipase medium was measured 
with a ruler. 53 of them (34 %) 10 mm and less, 99 of them (63 %) 20 mm and less, 4 of them (3 %) more than 20 mm 
transparent zone produced. The isolate which showed the highest lipase activity molecular identification has been made 
which based on 16S rRNA gene sequence. The 16S rRNA sequence information obtained from the isolate was recorded 
to GenBank with the access number KY172825. As a result of the study has been made on the NCBI database by using 
BLAST program, it was observed that, isolate showed 97% similarity ‘uncultured actinobacterium clone AS42 16S 
ribosomal RNA gene’ which’s access number on the database is EU283368.1.
AU  - ARIK, Gizem
AU  - Yılmaz Sarıözlü, Nalan
DO  - 10.46309/biodicon.2021.988928
PY  - 2021
JO  - Biological Diversity and Conservation
VL  - 14
IS  - 3
SN  - 1308-5301
SP  - 487
EP  - 492
DB  - TRDizin
UR  - http://search/yayin/detay/513087
ER  -