IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM 
AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION

yüksek, veysel; ÇETIN, SEDAT; USTA, AYŞE; Yaycı, Gülay; Dede, Semiha

doi:10.18038/aubtdc.802794

IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION

Gülay YAYCI, (Sağlık Bilimleri Üniversitesi, Van Eğitim ve Araştırma Hastanesi, Van, Türkiye)

Semiha DEDE, (Van Yüzüncü Yıl Üniversitesi, Veteriner Tıp Fakültesi, Biyokimya Anabilim Dalı, Van, Türkiye)

Sedat ÇETİN, (Van Yüzüncü Yıl Üniversitesi, Veteriner Tıp Fakültesi, Biyokimya Anabilim Dalı, Van, Türkiye)

AYŞE USTA, (Van Yüzüncü Yıl Üniversitesi, Fen Fakültesi, Kimya Bölümü, Van, Türkiye)

Veysel YÜKSEK (Van Yüzüncü Yıl Üniversitesi, Özalp Meslek Yüksekokulu, Van, Türkiye)

Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online)

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Yıl: 2021 Cilt: 10 Sayı: 2 Sayfa Aralığı: 132 - 137 Metin Dili: İngilizce DOI: 10.18038/aubtdc.802794 İndeks Tarihi: 04-06-2022

IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION

Öz:

This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent apoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated by regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 mM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and analyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using commercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. In GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which is the indicator of oxidative DNA damage.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Yaycı G, Dede S, ÇETIN S, USTA A, yüksek v (2021). IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION. , 132 - 137. 10.18038/aubtdc.802794
Chicago	Yaycı Gülay,Dede Semiha,ÇETIN SEDAT,USTA AYŞE,yüksek veysel IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION. (2021): 132 - 137. 10.18038/aubtdc.802794
MLA	Yaycı Gülay,Dede Semiha,ÇETIN SEDAT,USTA AYŞE,yüksek veysel IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION. , 2021, ss.132 - 137. 10.18038/aubtdc.802794
AMA	Yaycı G,Dede S,ÇETIN S,USTA A,yüksek v IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION. . 2021; 132 - 137. 10.18038/aubtdc.802794
Vancouver	Yaycı G,Dede S,ÇETIN S,USTA A,yüksek v IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION. . 2021; 132 - 137. 10.18038/aubtdc.802794
IEEE	Yaycı G,Dede S,ÇETIN S,USTA A,yüksek v "IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION." , ss.132 - 137, 2021. 10.18038/aubtdc.802794
ISNAD	Yaycı, Gülay vd. "IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION". (2021), 132-137. https://doi.org/10.18038/aubtdc.802794

APA	Yaycı G, Dede S, ÇETIN S, USTA A, yüksek v (2021). IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online), 10(2), 132 - 137. 10.18038/aubtdc.802794
Chicago	Yaycı Gülay,Dede Semiha,ÇETIN SEDAT,USTA AYŞE,yüksek veysel IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online) 10, no.2 (2021): 132 - 137. 10.18038/aubtdc.802794
MLA	Yaycı Gülay,Dede Semiha,ÇETIN SEDAT,USTA AYŞE,yüksek veysel IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online), vol.10, no.2, 2021, ss.132 - 137. 10.18038/aubtdc.802794
AMA	Yaycı G,Dede S,ÇETIN S,USTA A,yüksek v IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online). 2021; 10(2): 132 - 137. 10.18038/aubtdc.802794
Vancouver	Yaycı G,Dede S,ÇETIN S,USTA A,yüksek v IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online). 2021; 10(2): 132 - 137. 10.18038/aubtdc.802794
IEEE	Yaycı G,Dede S,ÇETIN S,USTA A,yüksek v "IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION." Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online), 10, ss.132 - 137, 2021. 10.18038/aubtdc.802794
ISNAD	Yaycı, Gülay vd. "IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION". Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online) 10/2 (2021), 132-137. https://doi.org/10.18038/aubtdc.802794