TY  - JOUR
TI  - IN VITRO EVALUATION OF THE EFFECT OF GLUTATHIONE ON CASPASE SYSTEM 
AND OXIDATIVE DNA DAMAGE IN HIGH GLUCOSE CONDITION
AB  - This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent 
apoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated 
by regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 
mM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and 
analyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using 
commercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. 
In GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which 
is the indicator of oxidative DNA damage.
AU  - yüksek, veysel
AU  - ÇETIN, SEDAT
AU  - USTA, AYŞE
AU  - Yaycı, Gülay
AU  - Dede, Semiha
DO  - 10.18038/aubtdc.802794
PY  - 2021
JO  - Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online)
VL  - 10
IS  - 2
SN  - 2667-4203
SP  - 132
EP  - 137
DB  - TRDizin
UR  - http://search/yayin/detay/514138
ER  -