Yıl: 2022 Cilt: 10 Sayı: 1 Sayfa Aralığı: 50 - 57 Metin Dili: İngilizce DOI: 10.33715/inonusaglik.1012011 İndeks Tarihi: 29-07-2022

ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT

Öz:
Metallic nanoparticles are important substances in medicine. These particles could be prepared by various ways including green synthesis. The production of silver nanoparticles by plant extracts is widely used because of their environmentally friendly properties. In this study, silver nanoparticles have been synthesized by biological method and Apricot tree (Prunus armeniaca) leaf extract was used as reducing agent. The color change and formation of the characteristic absorption peak at 400-500 nm were the first indications of AgNP formation. XRD analysis showed the crystalline particles and obtained AgNPs were generally in spherical shape with the average particle size of 24 nm. Their antimicrobial activity studies against various pathogenic microorganisms showed that they have strong antibacterial activity against Escherichia coli and Staphylococcus aureus and antifungal activity against Candida albicans. The minimum inhibitory concentrations for E. coli, S. aureus and C. albicans were 0.125, 0.125 and 0.250 g/mL, respectively. The results confirmed that Prunus armeniaca leaf extract could be used to produce AgNPs with efficient antimicrobial activity.
Anahtar Kelime: Green Synthesis Silver nanoparticles Antimicrobial Apricot Prunus armeniaca leaf extract

HEALTH SERVICES VOCATIONAL SCHOOL STUDENTS’ KNOWLEDGE LEVELS OF ON PARASITIC DISEASES

Öz:
In this study, it has been aimed to determine the knowledge level of the students of Van Yuzuncu Yıl University Health Services Vocational School, who will serve as the health personnel of the future, about parasitic diseases, prevention and control measures from these diseases. A total of 587 students, comprising 418 (71.2%) females and 169 (28.8%) males, studying at Van Yüzüncü Yıl University Health Services Vocational School, were included in the study. The questions in the questionnaire were arranged in three groups, as those about parasitic diseases, transmission routes of these diseases, and prevention and treatment. Cronbach alpha coefficients were calculated within the scope of the reliability analysis of the questionnaire questions. The Cronbach alpha values for the domains of Knowledge Level About Parasitic Diseases, Knowledge Level About the Transmission of Parasitic Diseases, and Knowledge Level About the Prevention and Treatment of Parasitic Diseases were determined as 0.647 (64.7%), 0.707 (70.7%), and 0.566 (56.6%), respectively. A statistically significant difference was found between the departments included in the study in terms of the level of knowledge about parasitic diseases (P < 0.05). A statistically significant difference was found between the departments in the evaluation made between the level of knowledge about the prevention and treatment of parasitic diseases and the personal characteristics of the students (P < 0.05). As the result, it was observed that the students participating in the study did not have sufficient knowledge about parasitic diseases.
Anahtar Kelime: Prevention Health Transmission Parasite

Sağlık Hizmetleri Meslek Yüksekokulu Öğrencilerinin Paraziter Hastalıklar Hakkındaki Bilgi Düzeyleri

Öz:
Bu çalışmada, geleceğin sağlık personeli olarak hizmet verecek olan Van Yüzüncü Yıl Üniversitesi Sağlık Hizmetleri Meslek Yüksekokulu öğrencilerinin paraziter hastalıklar, bu hastalıklardan korunma ve kontrol önlemleri hakkındaki bilgi düzeylerinin belirlenmesi amaçlanmıştır. Van Yüzüncü Yıl Üniversitesi Sağlık Hizmetleri Meslek Yüksekokulunda okuyan 418’i (%71.2) kız, 169’u (%28.8) erkek, toplam 587 öğrenci çalışamaya dâhil edildi. Anketteki sorular, paraziter hastalıklar, bu hastalıkların bulaş yolları, koruma ve tedavi hakkında olmak üzere üç grup olarak ayarlandı. Anket sorularının güvenirlik (reliability) analizi kapsamında Cronbach’s Alpha katsayıları hesaplandı. “Paraziter Hastalıklara İlişkin Bilgi Düzeyi”, “Paraziter Hastalıkların Bulaş Yollarına İlişkin Bilgi Düzeyi” ve “Paraziter Hastalıklardan Korunma ve Tedaviye İlişkin Bilgi Düzeyi” sorularına ait Cronbach’s Alpha değerleri sırasıyla 0.647 (%64.7), 0.707 (%70.7), 0.566 (%56.6) olarak saptandı. Çalışmaya dâhil edilen bölümler arasında, paraziter hastalıklara ilişkin bilgi düzeyi yönünden, istatistiksel olarak anlamlı bir fark bulundu (p<0.05). Paraziter hastalıklardan korunma ve tedaviye ilişkin bilgi düzeyi ile öğrencilerin kişisel özellikleri arasında yapılan değerlendirmede bölümler arasında istatistiksel olarak anlamlı bir fark bulundu (p<0.05). Sonuç olarak çalışmaya katılan öğrencilerin, paraziter hastalıklar hakkında yeterli bilgiye sahip olmadıkları gözlemlendi.
Anahtar Kelime:

Kayısı Yaprak Özütü ile Sentezlenen Gümüş Nanopartiküllerin Antimikrobiyal Potansiyeli

Öz:
Metalik nanopartiküller tıpta önemli maddelerdir. Bu partiküller yeşil sentez dahil çeşitli yollarla üretilebilirler. Gümüş nanopartiküllerin bitki özütleri ile üretimi çevre dostu özelliklerinden dolayı yaygın olarak kullanılmaktadır. Bu çalışmada gümüş nanopartiküller biyolojik yöntemle sentezlenmiş ve Kayısı ağacı (Prunus armeniaca) yaprak özütü redükleyici ajan olarak kullanılmıştır. Renk değişimi ve 400-500 nm’de özgül absorbans piki oluşumu AgNP oluşumunun ilk göstergeleri olmuştur. XRD analizi kristal yapıda partikülleri göstermiştir ve elde edilen AgNP’ler ortalama 24 nm boyutunda sferik şekillidir. Nanopartiküllerin çeşitli patojenik mikroorganizmalar üzerine antimikrobiyal aktivite çalışmaları, bu nanopartiküllerin Escherichia coli ve Staphylococcus aureus üzerine kuvvetli antibakteriyel ve Candida albicans’a karşı da kuvvetli antifungal etkisi olduğunu göstermiştir. E. coli, S. aureus ve C. albicans için minimum inhibe edici konsantrasyonlar sırasıyla 0.125, 0.125 ve 0.250 g /mL’dir. Sonuçlar, Prunus armeniaca yaprak özütünün etkili antimikrobiyal aktiviteye sahip AgNP’lerin üretiminde kullanılabileceğini ortaya koymuştur.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Aschale, A., Adane, M., Getachew, M., Faris, K., Gebretsadik, D., Sisay, T., ... Kloos, H. (2021). Water, sanitation, and hygiene conditions and prevalence of intestinal parasitosis among primary school children in Dessie City, Ethiopia. PloS one, 16(2), e0245463.
  • Ali, Z. A., Yahya, R., Sekaran, S. D., Puteh, R. (2016). Green synthesis of silver nanoparticles using apple extract and its antibacterial properties. Advances in Materials Science and Engineering, Volume 2016, Article ID 4102196, 1-6.
  • Aytar, A. A., Öztürk, E. C., Göçmen, Ş., Çalışkan, E., Özaras, F., Avcıoğlu, F., … Yavuz, M. T (2015). Investigation of intestinal parasites and level of hygiene knowledge of school students. Turkiye Parazitol Derg, 39, 277-285.
  • Apohan, E., Yilmaz, U., Yilmaz, O., Serindag, A., Kucukbay, H., Yesilada, O., Baran, Y. (2017). Synthesis, cytotoxic and antimicrobial activities of novel cobalt and zinc complexes of benzimidazole derivatives, Journal of Organometallic Chemistry. 828, 52-58.
  • Balcı, Y. I., Türk, M., Polat, Y., Erbil, N. (2009). The distrubition of intestinal parasites among children in Denizli. Türkiye Parazitoloji Derg, 33(4), 298-300.
  • Bao, Z. Q., Cao, J. H., Kang, G. B., Lan, C. Q. (2019). Effects of reaction conditions on light-dependent silver nanoparticle biosynthesis mediated by cell extract of green alga Neochloris oleoabundans. Environmental Science and Pollution Research, 26, 2873-2881.
  • Börekçi, G., Üzel, A. (2009). The determination of intestinal parasites, physical growth and hygiene behaviors of children in the Mersin city social service child care centre, Türkiye Parazitol Derg, 33, 63-72.
  • Chintamani, R. B., Salunkhe, K. S., Chavan, M. J. (2018). Emerging use of green synthesis silver nanoparticle: An updated review. International Journal of Pharmeceutical Sciences and Research, 9, 4029-4055.
  • Çaycı, T. Y., Hacıeminoğl, K., Birinci, A. (2017). Distribution of intestinal parasites detected in the laboratory of parasitology in Ondokuz Mayis University faculty of medicine hospital between 2014 and 2016. Journal of Health Sciences of Kocaeli University, 3(3), 6-8.
  • Dauthal, P., Mukhopadhyay, M. (2013). In-vitro free radical scavenging activity of biosynthesized gold and silver nanoparticles using Prunus armeniaca (apricot) fruit extract. Journal of Nanoparticle Research, 15, 1-11.
  • Cengiz, Z., Beyhan, Y., Çiçek, M., Yilmaz, H. (2015). Intestinal and hepatic parasites determined in a university hospital parasitology laboratory. Dicle Medical Journal, 42(3), 350-354.
  • Gopinath, K., Devi, N. P., Govindarajan, M., Bhakyaraj, K., Kumaraguru, S., Arumugam, A., Alharbi, N. S., Kadaikunnan, S., Benelli, G. (2017). One-Pot green synthesis of silver nanoparticles using the orchid leaf extracts of Anoectochilus elatus: Growth inhibition activity on seven microbial pathogens. Journal of Cluster Science, 28, 1541-1550.
  • Değerli, S., Özçelik, S., Çeliksöz, A. (2005). Distribution of intestinal parasites in patients admitted to Cumhuriyet University Faculty of Medicine Parasitology laboratory. Türkiye Parazitol Derg, 29(2), 116-9.
  • Gurunathan, S., Han, J. W., Kwon, D. N., Kim, J. H. (2014). Enhanced antibacterial and anti-biofilm activities of silver nanoparticles against Gram-negative and Gram-positive bacteria. Nanoscale Research Letters, 9, 1-17.
  • Demir, C., Yıldız, H., Yürektürk, Ş. (2020). Van Yuzuncu Yıl University Vocational School of Health Services students' knowledge level on infectious diseases. Van Med J., 27(4), 458-65.
  • Gurunathan, S., Raman, J., Malek, N. A., John, P. A., Vikineswary, S. (2013). Green synthesis of silver nanoparticles using Ganoderma neo-japonicum Imazeki: a potential cytotoxic agent against breast cancer cells. International Journal of Nanomedicine, 8, 4399-4413.
  • Doğan, N., Akdaş, İ., Gitmez, F., Ünsal, A. (2012). Knowledge level of the students in vocational school of health about parasitic diseases, Kafkas Univ Vet Fak Derg, 18, A71-A5.
  • Khan, A. U., Malik, N., Khan, M., Cho, M., Khan, M. M. M. (2018). Fungi-assisted silver nanoparticle synthesis and their applications. Bioprocess and Biosystem Engineering, 41, 1-20.
  • Eraslan, B. (2011). Evaluation of elemantary school teachings students’ candidates of about common communicable diseases behaviors of health protection. Bibad, 4(2), 17-22.
  • Logeswari, P., Silambarasan, S., Abraham, J. (2015). Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property. Journal of Saudi Chemical Society, 19, 311-317.
  • Gökkoca, Z. (2001). Sağlık eğitimi açısından temel ilkeler. Sürekli Tıp Eğitimi Dergisi, 10(10), 371-374.
  • Lopes, C. R. B., Courrol, L. C. (2018). Green synthesis of silver nanoparticles with extract of Mimusops coriacea and light. Journal of Luminescence, 199, 183-187.
  • Gülmez, D., Sarıbaş, Z., Akyön, Y., Ergüven, S. (2013). The results of Hacettepe University Faculty of Medicine Parasitology Laboratory in 2003-2012: evaluation of 10 years. Turkiye Parazitol Derg, 37, 97-101.
  • Mohammed, A. E., Al-Qahtani, A., Al-Mutairi, A., Al-Shamri, B., Aabed, K. (2018). Antibacterial and cytotoxic potential of biosynthesized silver nanoparticles by some plant extracts. Nanomaterials-Basel. 8, 1-15.
  • Sarı, C., Demirbağ, B. C. (2019). Assessment of knowledge level of parasitic diseases of health professionals working in pediatric services in a university hospital. Journal of Anatolia Nursing and Health Sciences, 22(3), 161-9.
  • Onitsuka, S., Hamada, T., Okamura, H. (2019). Preparation of antimicrobial gold and silver nanoparticles from tea leaf extracts. Colloids and Surfaces B, 173, 242-248.
  • Yan-Li, G., Yong-Gen, Z., Yi-Qing, X., Hong, C., Ming-Zhen, H., Lai-Fu, L., … Xiao-Lin, J. (2017). Investigation of major parasitic diseases and related knowledge, attitude and practice among rural residents in Liyang City. Chinese Journal Of Schistosomiasis Control, 30(1), 72-75.
  • Pirtarighat, S., Ghannadnia, M., Baghshahi, S. (2019). Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. Journal of Nanostructure in Chemistry, 9, 1-9.
  • Yılmazel, G., Yapar, D., Özkan, A. T. (2019). Knowledge and attitudes of nursing students about cystic hydatid in Çorum Province. Turk Hij Den Biyol Derg, 76(4), 431-40.
  • Raj, S., Mali, S. C., Trivedi, R. (2018). Green synthesis and characterization of silver nanoparticles using Enicostemma axillare (Lam.) leaf extract. Biochemical and Biophysical Reserach Communications, 503, 2814-2819.
  • Zakai, H. A. (2007). Level of awareness about parasitic diseases among students, Jeddah, Saudi Arabia. Journal of King Abdulaziz University-Medical Sciences, 14(2), 37-47.
  • Rajakumar, G., Gomathi, T., Thiruvengadam, M., Rajeswari, V. D., Kalpana, V. N., Chung, I. M. (2017). Evaluation of anti-cholinesterase, antibacterial and cytotoxic activities of green synthesized silver nanoparticles using from Millettia pinnata flower extract. Microbial Pathogenesis, 103, 123-128.
  • Ramanathan, S., Gopinath, S. C. B., Anbu, P., Lakshmipriya, T., Kasim, F. H., Lee, C. G. (2018). Eco-friendly synthesis of Solanum trilobatum extract-capped silver nanoparticles is compatible with good antimicrobial activities. Journal of Molecular Structure, 1160, 80-91.
  • Sharma, P., Pant, S., Rai, S., Yadav, R. B., Dave, V. (2018). Green Synthesis of silver nanoparticle capped with Allium cepa and their catalytic reduction of textile dyes: An ecofriendly approach. Journal of Polymers and The Environonment, 26, 1795-1803.
  • Singh, P., Kim, Y. J., Singh, H., Wang, C., Hwang, K. H., Farh, M. E. A., Yang, D. C. (2015). Biosynthesis, characterization and antimicrobial applications of silver nanoparticles. International Journal of Nanomedicine, 10, 2567-2577.
  • Veerasamy, R., Xin, T. Z., Gunasagaran, S., Xiang, T. F. W., Yang, E. F. C., Jeyakumar, N., Dhanaraj, S. A. (2011). Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. Journal of Saudi Chemical Society, 15, 113-120.
  • Velammal, S. P., Devi, T. A., Amaladhas, T. P. (2016). Antioxidant, antimicrobial and cytotoxic activities of silver and gold nanoparticles synthesized using Plumbago zeylanica bark. Journal of Nanostructure in Chemistry, 6, 247-260.
  • Venugopal, K., Rather, H. A., Rajagopal, K., Shanthi, M. P., Sheriff, K., Illiyas, M., Rather, R. A., Manikandan, E., Uvarajan, S., Bhaskar, M., Maaza, M. (2017). Synthesis of silver nanoparticles (AgNPs) for anticancer activities (MCF 7 breast and A549 lung cell lines) of the crude extract of Syzygium aromaticum. Journal of Photochemistry and Photobiology B: Biology, 167, 282-289.
  • Wang, M. M., Zhang, W. J., Zheng, X. S., Zhu, P. Z. (2017). Antibacterial and catalytic activities of biosynthesized silver nanoparticles prepared by using an aqueous extract of green coffee bean as a reducing agent. Rsc Advances, 7, 12144-12149.
APA BARAN A, Ekici A, Yesilada O, YÜREKTÜRK Ş, ELASAN S, HALİDİ A, karakuş s, AYDEMIR S, Şahin M, Yasul M, YILMAZ H (2022). ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT. , 50 - 57. 10.33715/inonusaglik.1012011
Chicago BARAN Ayşe,Ekici Abdurrahman,Yesilada Ozfer,YÜREKTÜRK ŞEHRİBAN,ELASAN Sadi,HALİDİ AHMED GALİP,karakuş sinan,AYDEMIR SELAHATTIN,Şahin Maksut,Yasul Muhammed,YILMAZ Hasan ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT. (2022): 50 - 57. 10.33715/inonusaglik.1012011
MLA BARAN Ayşe,Ekici Abdurrahman,Yesilada Ozfer,YÜREKTÜRK ŞEHRİBAN,ELASAN Sadi,HALİDİ AHMED GALİP,karakuş sinan,AYDEMIR SELAHATTIN,Şahin Maksut,Yasul Muhammed,YILMAZ Hasan ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT. , 2022, ss.50 - 57. 10.33715/inonusaglik.1012011
AMA BARAN A,Ekici A,Yesilada O,YÜREKTÜRK Ş,ELASAN S,HALİDİ A,karakuş s,AYDEMIR S,Şahin M,Yasul M,YILMAZ H ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT. . 2022; 50 - 57. 10.33715/inonusaglik.1012011
Vancouver BARAN A,Ekici A,Yesilada O,YÜREKTÜRK Ş,ELASAN S,HALİDİ A,karakuş s,AYDEMIR S,Şahin M,Yasul M,YILMAZ H ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT. . 2022; 50 - 57. 10.33715/inonusaglik.1012011
IEEE BARAN A,Ekici A,Yesilada O,YÜREKTÜRK Ş,ELASAN S,HALİDİ A,karakuş s,AYDEMIR S,Şahin M,Yasul M,YILMAZ H "ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT." , ss.50 - 57, 2022. 10.33715/inonusaglik.1012011
ISNAD BARAN, Ayşe vd. "ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT". (2022), 50-57. https://doi.org/10.33715/inonusaglik.1012011
APA BARAN A, Ekici A, Yesilada O, YÜREKTÜRK Ş, ELASAN S, HALİDİ A, karakuş s, AYDEMIR S, Şahin M, Yasul M, YILMAZ H (2022). ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT. İnönü üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi, 10(1), 50 - 57. 10.33715/inonusaglik.1012011
Chicago BARAN Ayşe,Ekici Abdurrahman,Yesilada Ozfer,YÜREKTÜRK ŞEHRİBAN,ELASAN Sadi,HALİDİ AHMED GALİP,karakuş sinan,AYDEMIR SELAHATTIN,Şahin Maksut,Yasul Muhammed,YILMAZ Hasan ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT. İnönü üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi 10, no.1 (2022): 50 - 57. 10.33715/inonusaglik.1012011
MLA BARAN Ayşe,Ekici Abdurrahman,Yesilada Ozfer,YÜREKTÜRK ŞEHRİBAN,ELASAN Sadi,HALİDİ AHMED GALİP,karakuş sinan,AYDEMIR SELAHATTIN,Şahin Maksut,Yasul Muhammed,YILMAZ Hasan ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT. İnönü üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi, vol.10, no.1, 2022, ss.50 - 57. 10.33715/inonusaglik.1012011
AMA BARAN A,Ekici A,Yesilada O,YÜREKTÜRK Ş,ELASAN S,HALİDİ A,karakuş s,AYDEMIR S,Şahin M,Yasul M,YILMAZ H ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT. İnönü üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi. 2022; 10(1): 50 - 57. 10.33715/inonusaglik.1012011
Vancouver BARAN A,Ekici A,Yesilada O,YÜREKTÜRK Ş,ELASAN S,HALİDİ A,karakuş s,AYDEMIR S,Şahin M,Yasul M,YILMAZ H ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT. İnönü üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi. 2022; 10(1): 50 - 57. 10.33715/inonusaglik.1012011
IEEE BARAN A,Ekici A,Yesilada O,YÜREKTÜRK Ş,ELASAN S,HALİDİ A,karakuş s,AYDEMIR S,Şahin M,Yasul M,YILMAZ H "ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT." İnönü üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi, 10, ss.50 - 57, 2022. 10.33715/inonusaglik.1012011
ISNAD BARAN, Ayşe vd. "ANTIMICROBIAL POTENTIAL OF SILVER NANOPARTICLES PRODUCED BY APRICOT LEAF EXTRACT". İnönü üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi 10/1 (2022), 50-57. https://doi.org/10.33715/inonusaglik.1012011