Yıl: 2005 Cilt: 29 Sayı: 3 Sayfa Aralığı: 171 - 176 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Echinococcus cinsinin moleküler genetik karakterizasyonu

Öz:
Echinococcus cinsinin, erişkin ve metacestod safhalarının; morfolojik, biyolojik ve epidemiyolojik özellikleri tür karakterizasyonunda en çok kullanılan kriterlerdir. Fakat suş tayini daha karmaşık olup morfolojik, biyolojik, biyokimyasal, epidemiyolojik ve moleküler kriterlere bağlıdır. Morfolojik ve biyolojik çalışmalar suş tayininde çok faydalı bilgiler sağlamasına rağ¬men, bu özelliklerin değişken olabileceği göz önüflde bulundurulmalıdır. Bunlar konak ve çevresel faktörlerden etkilenebilir ve genetik düzeydeki farklılığı yansıtmayabilirler. Klasik teknikler çevreden ve konaktan etkilenmesine rağmen, moleküler teknikler parazit genomunun direk karakterizasyonuna olanak sağlar ve çevresel faktörlerden etkilenmezler. Bu derlemede Echinococcus cinsinin tiplendirilmesinde kullanılan moleküler teknikler özetlenmiştir.
Anahtar Kelime: Polimeraz zincir reaksiyonu Polimorfizm, kesim parçacığı uzunluk Echinococcus Rastgele genişletilmiş polimorfik DNA tekniği

Konular: Genel ve Dahili Tıp Parazitoloji

Molecular genetic characterization of genus echinococcus

Öz:
Morphological, biological and epidemiological features of the adult and metacestode stage of the genus Echinococcus have been the most frequently used criteria for the characterization of species. But strain characterization is more complex and is based on the morphological, biological, biochemical, epidemiological and molecular features. Although morphological and biological studies have provided extremely useful information for strain characterization, that these features may be variable must be taken into consideration. They may be influenced by host and environmental factors and may not reflect distinctness at a genetic level. Although, conventional techniques may be affected by the environment or host, molecular techniques allow a direct characterization of the genome of the parasite and they are not affected by environmental factors. In this review, various data concerning molecular typing methods of the genus Echinococcus have been summarized.
Anahtar Kelime: Echinococcus Random Amplified Polymorphic DNA Technique Polymerase Chain Reaction Polymorphism, Restriction Fragment Length

Konular: Genel ve Dahili Tıp Parazitoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1. Amman RW, Eckcrt J, 1995. Clinical diagnosis and treatment of echinococcosis in humans. Thompson RCA. Lymbery AJ Eds. Echinococcus and Hydatid Disease. Wallingford, Oxon:CAB International, p. 411-463.
  • 2. Anon. RAPDs. İnternet erişim adresi. http://www.usask.ca/agriculture/plantsci/classes/plsc416/projects _2002/pawlin/resources
  • 3. Anon. SSCP (Single Stranded Conformational Polymorphism). Internet erişim adresi. http//www.egn.wageningenur.nl/pgr/research/molgen/sses.htm
  • 4. Arda M, 1994. Biyoteknoloji (Bazı Temel İlkeler). İkinci baskı. Ankara: Kükem Derneği Bilimsel Yayınlan. No:2
  • 5. Bowles J, Blair D, McManus DP, 1992. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol., 54: 165-173.
  • 6. Bowles J, Blair D, McManus DP, 1994. Molecular genetic characterization of the cervid strain (" northern form") of Echinococcus granulosus. Parasitology, 109(2): 215-221.
  • 7. Bowles J, McManus DP, 1993. Molecular variation in Echinococcus. Açta Trop., 53: 291-305.
  • 8. Bowles J, McManus DP, 1993. Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction- . based RFLP method. Mol Biochem Parasitol., 57: 231-239.
  • 9. Bowles J, McManus DP, 1993. NADH dehydrogenase I gene sequences compared for species and strains of the genus Echinococcus. hit J Parasitol. ,23: 969-972.
  • 10. Cotton RGH, 1997. Mutation Detection. Oxford : Oxford University Pres.
  • 11. Eckert J, Thompson RCA, 1997. Intraspesific variation of Echinococcus granulosus and- related species with emphises on their infecdvity to humans. Acta Tropica, 64: 19-34.
  • 12. Ellsworth DL, Rittenhouse KD, Honeycutt RL 3. Artifactual variation in randomly amplified polymorphic UNA banding patterns. Bio Techniques, 14(2): 214-217.
  • 13- Gasser RB, 1997. Mutation scaning methods for the analysis of parasite genes, bit J Parasitol., 27: 1449-1463.
  • 14. Gasser RB, 1999. PCR-based technology in veterinary parasitology. Vet Parasitol., 84: 229-258.
  • 15. Gasser RB, Monti JR, 1997. Identification of parasitic nematodes by PCR-SSCP of ITS-2 rDNA. Mol Cell Prob 11,201-209.
  • 16. Gasser RB, Zhu X, McManus DP, 1998. Dideoxy fingerprinting: application to the genotyping of Echinococcus.Int J Parasitol, 28: 1775-1779.
  • 17. Gasser RB, Zhu X, McManus DP, 1998. Display of sequence variation in PCR-amplified mitochondrial DNA regions of Echinococcus by single-strand conformation polymorphism. Acta Tropica, 71: 107-115.
  • 18. Gonzalez LM, Daniel-Mwambete K, Montero E, Rosenzvit MC, McManus DP, Gârate T, Cuesta-Bandera C, 2002. Further molecular discrimination of Spanish strains of Echinococcus granulosus. Exp Parasitol., 102: 46-56.
  • 19. Güralp N, 1981. Helmintoloji. İkinci baskı. Ank. Üniv. Vet Fak. Yayın. No:368. Ankara
  • 20. Haag KL, Araüjo AM, Gottstein B, Zaha A, 1998. Selection,recombination and history in a parasitic flatworm (Echinococcus) inferred from nucleotide sequences. Mem Inst Oswaldo Cruz., 93(5): 695-702.
  • 21. Haag KL, Zaha A, Araujo AM, Gottstein B, 1997. Reduced genetic variability within coding and non-coding regions of the Echinococcus multilocularis genome. Parasitology, 115: 521-529
  • 22. Harandi MF, Hobbs RP, Adams PJ, Mobedi I, Morgan-Ryan UM, Thompson RCA, 2002. Molecular and morphological characterization of Echinococcus granulosus of human and animal origin in Iran. Parasitology, 125: 367-373
  • 23. Hobbs RP, Lymbery AJ, Thompson RCA, 1990. Rostellar hook morphology of Echinococcus granulosus (Batsch, 1786) from natural and experimental Australian hosts, and its implications for strain recognition. Parasitology, 101(2): 273-281.
  • 24. Hongyo T, Buzard GS, Calvert RJ, Weghorst CM, 1993. Cold SSCP: a simple, rapid and non-radyoactive method for optimized single strand conformation polymorphism, analyses. Nucleic Acid Research, 21: 3637-3642.
  • 25. Kamenetzky L, Gutierrez AM, Canova SG, Haag KL, Guarnera EA, Parra A, Garcia GE, Rosenzvit MC, 2002. Several strains of Echinococcus granulosus infect livestock and humans in Argentina. Infection Genetics and Evolution, 2: 129-136.
  • 26. Lavikainen A, Lehtinen MJ, Meri T, Hirvela-Koski V, Meri S, 2003. Molecular genetic characterization of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus. Parasitology, 127(3): 207-215
  • 27. Le TH, Pearson MS, Blair D, Dai N, Zhang LH, McManus DP, 2002. Complete mitochondrial genomes confirm the distinctivenes of the horse-dog and sheep-dog strains of Echinococcus granulosus, Parasitology, 124: 97-112.
  • 28. MacPherson JM, Eckstein PE, Scoles GJ, Gajadhar AA,1993. Variability of the random amplified polymorphic DNA assay among thermal cyclers, and effects of primer and DNA concentration. Molecular and Cellular Probes, 7(4). 293-294.
  • 29. McManus DP, 2002. The molecular epidemiology of echinococcus granulosus and cystic hydatid disease. Trans R Sac Trap Med Hyg., 96(1): 151-157.
  • 30. McManus DP, Ding Z, Bowles J, 1994. A molecular genetic survey indicates the presence of a single, homogeneous strain of Echinococcus granulosus in north-western China. Ada Trap,56(1): 7-14.
  • 31. Melchcr U, 2003. SSCPs http://opbs.okstate.edu/~melcher/MG/MGWl/MGl 1129.html.
  • 32. Nikulina NA, Benediktov H, Garaev MM, 2003. Echinococcus genotyping by PCR-RFLP. Med Parasitol., 2:29-32.
  • 33. Olive M, Bean P, 1999. Principles and applications of methods for DNA based typing of microbial organisms. J Clin MicrobioL, 37: 1661-1669.
  • 34. Orita M, Suzuki Y, Sekiya T, Hayashi K, 1989. Rapid and sensitive detection! of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics, 5(4): 874-879.
  • 35. Ortona E, Margutti P, Rigano R, Siracusano A, 1996. Genetic variabilty in Italian sheep isolates of Echinococcus granulosus. Appl Parasitol., 3: 205-208.
  • 36. Ralp D, McCleland M, 1998. Arbitrary primed PCR methods for studying bacterial disease. Woodforn N, Johnson P, Eds. Methods in Molecular Medicine. Humana Pres Inc, Totowa, NJ, p.60-75.
  • 37. Reddy YA, Rao JR, Butchaiah G, Sharma B, 1998. Random amplified polymorphic DNA for the specific detection of the bubaline Echinococcus gnmulosus by hybridization assay. Vet Parasitol.. 79:315-323.
  • 38. Rozenzvit MC, Zhang LH, Kamenetzky L, Canova SG, Guarnera EA, McManus DP, 1999. Genetic variation and epidemiology of Echinococcus granulosus in Argentina. Parasitology, 118(5): 523-530.
  • 39. Sarkar G, Yoon HS, Sommer SS, 1992. Dideoxy fingerprinting: a rapid and efficient screen for the presence of mutations. Genonfics, 13(2): 441-443.
  • 40. Scott JC, Stefaniak J, Pawlowski ZS, McManus DP, 1997. Molecular genetic analysis of human cystic hydatid cases from Poland: identification of a new genotipic group (G9) of Echinococcus granulosus. Parasitology, 114: 37-43.
  • 41. Sheffield VD, Beck JS, Kwitek AE, Sandstrom D, W&Stone EM, 1993. The sensitivity of single strand conformation polymorphism analysis for the detection of single base substitutions. Genomics, 16: 325-332.
  • 42. Siles-Lucas M, Benito MC, Cuesta-Bandera C, 1996. Echinococcus granulosus: genomic and izoenzymatic study of Spanish strains isolated from different intermediate hosts. Vet Parasitol., 63: 273-282.
  • 43. Siles-Lucas M, Felleisen R, Cuesta-Bandera C, Gottstein B, Eckert J, 1994. Comparative genetic analysis of Swiss and Spanish isolates of Echinococcus granulosus by southern hybridization and Random Amplified Polymorphic DNA technique. Appl Parasitol., 35: 107-117.
  • 44. Snabel V, D'Amelio S, Mathiopoulos K, Turcekova L, Dubinsky P, 2000. Molecular evidence for the presence of a G7 genotype of Echinococcus granulosus in Slovakia. J Helminth., 74(2): 177-181.
  • 45. Sunnucks P, Wilson ACC, Beheregaray LB, Zenger K, French J, Taylor AC, 2000. SSCP is not so difficult: the application and utility of single-stranded conformation polymorphism in evolutionary biology and molecular ecology. Molecular Ecology, 9: 1699-1710.
  • 46. Thompson RCA, 1998. Species and strains in the genus £6/HH0ONXM.v-epidemiologica! and evolutionary perspectives. Parasit Int., 47: 133-281.
  • 47. Thompson RCA, Lymbery AJ, 1988. The nature, extent and significance of variation within the genus Echinococcus. Adv Parasitol., 27:209-258.
  • 48. Thompson RCA, Lymbery AJ, Constantine CC, 1995. Variation in Echinococcus: towards a taxonomic revision of the genus. Adv Parasitol., 35: 145-176.
  • 49. Thompson RCA, McManus DP, 2002. Towards a taxonomic revision of the genus Echinococcus. Trends in.. Parasitology, 18(10): 452-457.
  • 50. Travis Glenn, 1996. Step by step SSCP. http://www.uga.edu/srel/DNA_Lab/SSCP'96V2.rtf.
  • 51. Turcekova L, Snâbel V, D'Amelio S, Busi M., Dubinsky P, 2003. Morphological and genetic characterization of Echinococcus granulosus in the Slovak Republic. Ada Tropica., 85: 223-229.
  • 52. Xue HC, Qiu LS, Zhu CW, 1993. RFLP analysis of DNA from Echinococcus granulosus collected from four provinces/autonomous region in China. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chang Bing Za Zhl, 11(3): 201-203.
  • 53. Yağcı A, 2001. Restriction Fragment Length Polymorphism ve Polimeraz Zincir Reaksiyon Bazlı Tiplendirme Yöntemleri.Durmaz R, Ed. Uygulamalı Moleküler Mikrobiyoloji, 2.baskı,Malatya: İnönü Üniv.Tıp Fak. Mikrobiyoloji veJ Klinik Mikrobiyoloji AD, p. 149-160.
  • 54. Zhang L, Eslanıi A, Hosseini H, McManus DP, 1998. Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mitocondrial DNA markers. Am J Trop Med Hyg., 1998; 59: 171 -174.
  • 55. Zhang L, Gasser RB, Zhu X, McManus DP, 1999 Screening for different genotypes of Echinococcus granulosus within China and Argentina by single-strand conformation polymorphism (SSCP) analysis. Trans R Soc Trop Med Hyg.,93:329-334.
APA ÜTÜK A, Simsek S, KÖROĞLU E (2005). Echinococcus cinsinin moleküler genetik karakterizasyonu. , 171 - 176.
Chicago ÜTÜK Armağan Erdem,Simsek Sami,KÖROĞLU Ergün Echinococcus cinsinin moleküler genetik karakterizasyonu. (2005): 171 - 176.
MLA ÜTÜK Armağan Erdem,Simsek Sami,KÖROĞLU Ergün Echinococcus cinsinin moleküler genetik karakterizasyonu. , 2005, ss.171 - 176.
AMA ÜTÜK A,Simsek S,KÖROĞLU E Echinococcus cinsinin moleküler genetik karakterizasyonu. . 2005; 171 - 176.
Vancouver ÜTÜK A,Simsek S,KÖROĞLU E Echinococcus cinsinin moleküler genetik karakterizasyonu. . 2005; 171 - 176.
IEEE ÜTÜK A,Simsek S,KÖROĞLU E "Echinococcus cinsinin moleküler genetik karakterizasyonu." , ss.171 - 176, 2005.
ISNAD ÜTÜK, Armağan Erdem vd. "Echinococcus cinsinin moleküler genetik karakterizasyonu". (2005), 171-176.
APA ÜTÜK A, Simsek S, KÖROĞLU E (2005). Echinococcus cinsinin moleküler genetik karakterizasyonu. Türkiye Parazitoloji Dergisi, 29(3), 171 - 176.
Chicago ÜTÜK Armağan Erdem,Simsek Sami,KÖROĞLU Ergün Echinococcus cinsinin moleküler genetik karakterizasyonu. Türkiye Parazitoloji Dergisi 29, no.3 (2005): 171 - 176.
MLA ÜTÜK Armağan Erdem,Simsek Sami,KÖROĞLU Ergün Echinococcus cinsinin moleküler genetik karakterizasyonu. Türkiye Parazitoloji Dergisi, vol.29, no.3, 2005, ss.171 - 176.
AMA ÜTÜK A,Simsek S,KÖROĞLU E Echinococcus cinsinin moleküler genetik karakterizasyonu. Türkiye Parazitoloji Dergisi. 2005; 29(3): 171 - 176.
Vancouver ÜTÜK A,Simsek S,KÖROĞLU E Echinococcus cinsinin moleküler genetik karakterizasyonu. Türkiye Parazitoloji Dergisi. 2005; 29(3): 171 - 176.
IEEE ÜTÜK A,Simsek S,KÖROĞLU E "Echinococcus cinsinin moleküler genetik karakterizasyonu." Türkiye Parazitoloji Dergisi, 29, ss.171 - 176, 2005.
ISNAD ÜTÜK, Armağan Erdem vd. "Echinococcus cinsinin moleküler genetik karakterizasyonu". Türkiye Parazitoloji Dergisi 29/3 (2005), 171-176.