Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.)
plants after particle bombardment

Bakhsh, Allah; OZTÜRK GOKÇE, ZAHIDE NESLIHAN; Aksoy, Emre; Hossain, Md Jakir; Joyia, Faiz; Khan, Muhammad Sarwar

doi:10.3906/tar-2010-103

Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment

Md Jakir HOSSAIN, (Niğde Ömer Halisdemir Üniversitesi, Tarım Bilimleri ve Teknolojileri Fakültesi, Zirai Genetik Mühendisliği Bölümü, Niğde, Türkiye)

Emre AKSOY, (Niğde Ömer Halisdemir Üniversitesi, Tarım Bilimleri ve Teknolojileri Fakültesi, Zirai Genetik Mühendisliği Bölümü, Niğde, Türkiye)

Neslihan Zahide ÖZTÜRK GÖKÇE, (Niğde Ömer Halisdemir Üniversitesi, Tarım Bilimleri ve Teknolojileri Fakültesi, Zirai Genetik Mühendisliği Bölümü, Niğde, Türkiye)

Faiz Ahmad JOYIA, (University of Agriculture Faisalabad, Center of Agricultural Biochemistry and Biotechnology (CABB), Faisalabad, Pakistan)

Muhammad Sarwar KHAN, (University of Agriculture Faisalabad, Center of Agricultural Biochemistry and Biotechnology (CABB), Faisalabad, Pakistan)

Allah BAKHSH (Niğde Ömer Halisdemir Üniversitesi, Tarım Bilimleri ve Teknolojileri Fakültesi, Zirai Genetik Mühendisliği Bölümü, Niğde, Türkiye)

Turkish Journal of Agriculture and Forestry

8 0

Yıl: 2021 Cilt: 45 Sayı: 3 Sayfa Aralığı: 313 - 323 Metin Dili: İngilizce DOI: 10.3906/tar-2010-103 İndeks Tarihi: 06-06-2022

Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment

Öz:

In contrast with traditional nuclear gene transformation, transplastomic technology has opened a new horizon for the transgenic plant research that offers several beneficial aspects including the convenient use of transgene stacking and the generation of high expression levels of foreign proteins. However, this technology has been well adopted and established in tobacco, the introduction and adoption of cost-effective, swift, and reproducible protocol for in vitro regeneration of transplastomic potato is challenging and laborious. The present research aimed to develop such prompt and efficient protocol to instigate and revive the regeneration potential with the combinations of different plant growth regulators (PGRs). Leaves and internodal explants from four potato cultivars were transformed with chloroplast transformation vector via particle bombardment and cultured on MS media supplemented with suitable PGRs and selection agents. Leaf explants of cultivar Kuroda induced highest (92%) number of calli where cultivar Sante produced the highest (85.7%) transplastomic shoots. Thidiazuron was found more proficient (41%) for shoot regeneration. Finally, within only seven weeks, we got 21 spectinomycin resistant shoot, and 16 of those showed integration of target genes into the plastome in PCR screening.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Hossain M, Aksoy E, OZTÜRK GOKÇE Z, Joyia F, Khan M, Bakhsh A (2021). Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment. , 313 - 323. 10.3906/tar-2010-103
Chicago	Hossain Md Jakir,Aksoy Emre,OZTÜRK GOKÇE ZAHIDE NESLIHAN,Joyia Faiz,Khan Muhammad Sarwar,Bakhsh Allah Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment. (2021): 313 - 323. 10.3906/tar-2010-103
MLA	Hossain Md Jakir,Aksoy Emre,OZTÜRK GOKÇE ZAHIDE NESLIHAN,Joyia Faiz,Khan Muhammad Sarwar,Bakhsh Allah Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment. , 2021, ss.313 - 323. 10.3906/tar-2010-103
AMA	Hossain M,Aksoy E,OZTÜRK GOKÇE Z,Joyia F,Khan M,Bakhsh A Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment. . 2021; 313 - 323. 10.3906/tar-2010-103
Vancouver	Hossain M,Aksoy E,OZTÜRK GOKÇE Z,Joyia F,Khan M,Bakhsh A Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment. . 2021; 313 - 323. 10.3906/tar-2010-103
IEEE	Hossain M,Aksoy E,OZTÜRK GOKÇE Z,Joyia F,Khan M,Bakhsh A "Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment." , ss.313 - 323, 2021. 10.3906/tar-2010-103
ISNAD	Hossain, Md Jakir vd. "Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment". (2021), 313-323. https://doi.org/10.3906/tar-2010-103

APA	Hossain M, Aksoy E, OZTÜRK GOKÇE Z, Joyia F, Khan M, Bakhsh A (2021). Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment. Turkish Journal of Agriculture and Forestry, 45(3), 313 - 323. 10.3906/tar-2010-103
Chicago	Hossain Md Jakir,Aksoy Emre,OZTÜRK GOKÇE ZAHIDE NESLIHAN,Joyia Faiz,Khan Muhammad Sarwar,Bakhsh Allah Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment. Turkish Journal of Agriculture and Forestry 45, no.3 (2021): 313 - 323. 10.3906/tar-2010-103
MLA	Hossain Md Jakir,Aksoy Emre,OZTÜRK GOKÇE ZAHIDE NESLIHAN,Joyia Faiz,Khan Muhammad Sarwar,Bakhsh Allah Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment. Turkish Journal of Agriculture and Forestry, vol.45, no.3, 2021, ss.313 - 323. 10.3906/tar-2010-103
AMA	Hossain M,Aksoy E,OZTÜRK GOKÇE Z,Joyia F,Khan M,Bakhsh A Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment. Turkish Journal of Agriculture and Forestry. 2021; 45(3): 313 - 323. 10.3906/tar-2010-103
Vancouver	Hossain M,Aksoy E,OZTÜRK GOKÇE Z,Joyia F,Khan M,Bakhsh A Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment. Turkish Journal of Agriculture and Forestry. 2021; 45(3): 313 - 323. 10.3906/tar-2010-103
IEEE	Hossain M,Aksoy E,OZTÜRK GOKÇE Z,Joyia F,Khan M,Bakhsh A "Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment." Turkish Journal of Agriculture and Forestry, 45, ss.313 - 323, 2021. 10.3906/tar-2010-103
ISNAD	Hossain, Md Jakir vd. "Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.) plants after particle bombardment". Turkish Journal of Agriculture and Forestry 45/3 (2021), 313-323. https://doi.org/10.3906/tar-2010-103