TY  - JOUR
TI  - Rapid and efficient in vitro regeneration of transplastomic potato (Solanum tuberosum L.)
plants after particle bombardment
AB  - In contrast with traditional nuclear gene transformation, transplastomic technology has opened a new horizon for the
transgenic plant research that offers several beneficial aspects including the convenient use of transgene stacking and the generation of
high expression levels of foreign proteins. However, this technology has been well adopted and established in tobacco, the introduction
and adoption of cost-effective, swift, and reproducible protocol for in vitro regeneration of transplastomic potato is challenging and
laborious. The present research aimed to develop such prompt and efficient protocol to instigate and revive the regeneration potential
with the combinations of different plant growth regulators (PGRs). Leaves and internodal explants from four potato cultivars were
transformed with chloroplast transformation vector via particle bombardment and cultured on MS media supplemented with suitable
PGRs and selection agents. Leaf explants of cultivar Kuroda induced highest (92%) number of calli where cultivar Sante produced the
highest (85.7%) transplastomic shoots. Thidiazuron was found more proficient (41%) for shoot regeneration. Finally, within only seven
weeks, we got 21 spectinomycin resistant shoot, and 16 of those showed integration of target genes into the plastome in PCR screening.
AU  - Bakhsh, Allah
AU  - OZTÜRK GOKÇE, ZAHIDE NESLIHAN
AU  - Aksoy, Emre
AU  - Hossain, Md Jakir
AU  - Joyia, Faiz
AU  - Khan, Muhammad Sarwar
DO  - 10.3906/tar-2010-103
PY  - 2021
JO  - Turkish Journal of Agriculture and Forestry
VL  - 45
IS  - 3
SN  - 1300-011X
SP  - 313
EP  - 323
DB  - TRDizin
UR  - http://search/yayin/detay/514457
ER  -