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Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.)

Selçuk KODAZ, (Atatürk Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Erzurum, Türkiye)
Kamil HALİLOĞLU, (Atatürk Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Erzurum, Türkiye)
Arash HOSSEIN POUR, (Atatürk Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Erzurum, Türkiye)
Murat AYDIN (Atatürk Üniversitesi, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü, Erzurum, Türkiye)
Turkish Journal of Agriculture and Forestry
3 0
Yıl: 2021 Cilt: 45 Sayı: 4 Sayfa Aralığı: 454 - 464 Metin Dili: İngilizce DOI: 10.3906/tar-1910-61 İndeks Tarihi: 06-06-2022

Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.)

Öz:
To establish an efficient in vitro regeneration protocol for orchardgrass (Dactylis glomerata L.), type of medium, several plant growth regulators and their various concentrations were evaluated in a factorial experiment for callus induction and plant regeneration. Endosperm supported mature embryo were used as explants and optimum response in callus induction was observed on Chu medium with 12 mg/L dicamba (3.6-dichloro-2-methoxybenzoic acid). The highest responding embryogenic callus (15%) was found with Murashige and Skoog medium and 8 mg/L 2.4-D (2,4-dichlorophenoxy acetic acid). The highest regeneration efficiency was achieved on MS medium (17 plantlets) with 8 mg/L 2,4-D. Endosperm-supported mature embryos of orchardgrass can be successfully used as explants. Therefore, this protocol provides a basis for future studies on genetic improvement and in vitro mutation studies.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA KODAZ S, Haliloglu K, Hossein pour A, AYDIN M (2021). Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.). , 454 - 464. 10.3906/tar-1910-61
Chicago KODAZ Selçuk,Haliloglu Kamil,Hossein pour Arash,AYDIN Murat Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.). (2021): 454 - 464. 10.3906/tar-1910-61
MLA KODAZ Selçuk,Haliloglu Kamil,Hossein pour Arash,AYDIN Murat Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.). , 2021, ss.454 - 464. 10.3906/tar-1910-61
AMA KODAZ S,Haliloglu K,Hossein pour A,AYDIN M Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.). . 2021; 454 - 464. 10.3906/tar-1910-61
Vancouver KODAZ S,Haliloglu K,Hossein pour A,AYDIN M Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.). . 2021; 454 - 464. 10.3906/tar-1910-61
IEEE KODAZ S,Haliloglu K,Hossein pour A,AYDIN M "Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.)." , ss.454 - 464, 2021. 10.3906/tar-1910-61
ISNAD KODAZ, Selçuk vd. "Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.)". (2021), 454-464. https://doi.org/10.3906/tar-1910-61
APA KODAZ S, Haliloglu K, Hossein pour A, AYDIN M (2021). Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.). Turkish Journal of Agriculture and Forestry, 45(4), 454 - 464. 10.3906/tar-1910-61
Chicago KODAZ Selçuk,Haliloglu Kamil,Hossein pour Arash,AYDIN Murat Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.). Turkish Journal of Agriculture and Forestry 45, no.4 (2021): 454 - 464. 10.3906/tar-1910-61
MLA KODAZ Selçuk,Haliloglu Kamil,Hossein pour Arash,AYDIN Murat Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.). Turkish Journal of Agriculture and Forestry, vol.45, no.4, 2021, ss.454 - 464. 10.3906/tar-1910-61
AMA KODAZ S,Haliloglu K,Hossein pour A,AYDIN M Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.). Turkish Journal of Agriculture and Forestry. 2021; 45(4): 454 - 464. 10.3906/tar-1910-61
Vancouver KODAZ S,Haliloglu K,Hossein pour A,AYDIN M Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.). Turkish Journal of Agriculture and Forestry. 2021; 45(4): 454 - 464. 10.3906/tar-1910-61
IEEE KODAZ S,Haliloglu K,Hossein pour A,AYDIN M "Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.)." Turkish Journal of Agriculture and Forestry, 45, ss.454 - 464, 2021. 10.3906/tar-1910-61
ISNAD KODAZ, Selçuk vd. "Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.)". Turkish Journal of Agriculture and Forestry 45/4 (2021), 454-464. https://doi.org/10.3906/tar-1910-61
Sayfa Oluşturulma Tarihi
23-04-2024 22:53

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