TY  - JOUR
TI  - Culture media and growth regulators influence callus induction and plant regeneration
of mature embryos of orchardgrass (Dactylis glomerata L.)
AB  - To establish an efficient in vitro regeneration protocol for orchardgrass (Dactylis glomerata L.), type of medium, several plant
growth regulators and their various concentrations were evaluated in a factorial experiment for callus induction and plant regeneration.
Endosperm supported mature embryo were used as explants and optimum response in callus induction was observed on Chu medium
with 12 mg/L dicamba (3.6-dichloro-2-methoxybenzoic acid). The highest responding embryogenic callus (15%) was found with
Murashige and Skoog medium and 8 mg/L 2.4-D (2,4-dichlorophenoxy acetic acid). The highest regeneration efficiency was achieved
on MS medium (17 plantlets) with 8 mg/L 2,4-D. Endosperm-supported mature embryos of orchardgrass can be successfully used as
explants. Therefore, this protocol provides a basis for future studies on genetic improvement and in vitro mutation studies.
AU  - Haliloglu, Kamil
AU  - KODAZ, Selçuk
AU  - Hossein pour, Arash
AU  - AYDIN, Murat
DO  - 10.3906/tar-1910-61
PY  - 2021
JO  - Turkish Journal of Agriculture and Forestry
VL  - 45
IS  - 4
SN  - 1300-011X
SP  - 454
EP  - 464
DB  - TRDizin
UR  - http://search/yayin/detay/514517
ER  -