Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars
Yıl: 2021 Cilt: 45 Sayı: 6 Sayfa Aralığı: 704 - 716 Metin Dili: İngilizce DOI: 10.3906/tar-2009-49 İndeks Tarihi: 06-06-2022
Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars
Öz: Tomato (Solanum lycopersicum) is the second most important horticultural crop worldwide that is widely used as a model
plant in genetic manipulation of Solanaceae. CRISPR/Cas9 system has been successfully utilized in several studies for genome edition
of model tomato cultivars. However, these genome editing systems should be also optimized for commercial tomato cultivar for direct
application of genome editing in field conditions. In this study, we have optimized an Agrobacterium-mediated gene transfer and
regeneration system for CRISPR/Cas9 genome editing in two commercial tomato cultivars for the first time. The effect of explant
type, genotype, pre-transformation time, Agrobacterium concentration, infection time, and different co-culture periods of bacteria were
evaluated to optimize the regeneration and transformation parameters. The highest regeneration capacity of 83% was obtained from
cotyledons of Crocker incubated in a medium supplemented with BA (3 mg/L) and IAA (0.1 mg/L). The maximum transformation
frequency was obtained by using the following parameters: cotyledon explants of commercial Crocker cultivar that were left for 2 days
of pre-transformation incubation, infected with Agrobacterium for 10 min at a concentration of OD600 of 0.6 and co-cultivated with
Agrobacterium cells for 48 h. CRISPR/Cas9 system was tested with two gRNAs targeting the phytoene desaturase gene. Fully albino and
chimeric plants were successfully produced with optimized transformation and culture conditions in up to 71% of all regenerated plants.
In the current study, we optimized the implementation of the CRISPR/Cas9 technique in a commercial tomato cultivar and our method
will enable breeders to make necessary changes in traits of interest to improve tomato crops for commercial applications.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | secgin z, KAVAS M, yıldırım k (2021). Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars. , 704 - 716. 10.3906/tar-2009-49 |
| Chicago | secgin zafer,KAVAS MUSA,yıldırım kubilay Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars. (2021): 704 - 716. 10.3906/tar-2009-49 |
| MLA | secgin zafer,KAVAS MUSA,yıldırım kubilay Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars. , 2021, ss.704 - 716. 10.3906/tar-2009-49 |
| AMA | secgin z,KAVAS M,yıldırım k Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars. . 2021; 704 - 716. 10.3906/tar-2009-49 |
| Vancouver | secgin z,KAVAS M,yıldırım k Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars. . 2021; 704 - 716. 10.3906/tar-2009-49 |
| IEEE | secgin z,KAVAS M,yıldırım k "Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars." , ss.704 - 716, 2021. 10.3906/tar-2009-49 |
| ISNAD | secgin, zafer vd. "Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars". (2021), 704-716. https://doi.org/10.3906/tar-2009-49 |
| APA | secgin z, KAVAS M, yıldırım k (2021). Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars. Turkish Journal of Agriculture and Forestry, 45(6), 704 - 716. 10.3906/tar-2009-49 |
| Chicago | secgin zafer,KAVAS MUSA,yıldırım kubilay Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars. Turkish Journal of Agriculture and Forestry 45, no.6 (2021): 704 - 716. 10.3906/tar-2009-49 |
| MLA | secgin zafer,KAVAS MUSA,yıldırım kubilay Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars. Turkish Journal of Agriculture and Forestry, vol.45, no.6, 2021, ss.704 - 716. 10.3906/tar-2009-49 |
| AMA | secgin z,KAVAS M,yıldırım k Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars. Turkish Journal of Agriculture and Forestry. 2021; 45(6): 704 - 716. 10.3906/tar-2009-49 |
| Vancouver | secgin z,KAVAS M,yıldırım k Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars. Turkish Journal of Agriculture and Forestry. 2021; 45(6): 704 - 716. 10.3906/tar-2009-49 |
| IEEE | secgin z,KAVAS M,yıldırım k "Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars." Turkish Journal of Agriculture and Forestry, 45, ss.704 - 716, 2021. 10.3906/tar-2009-49 |
| ISNAD | secgin, zafer vd. "Optimization of Agrobacterium-mediated transformation and regeneration for CRISPR/ Cas9 genome editing of commercial tomato cultivars". Turkish Journal of Agriculture and Forestry 45/6 (2021), 704-716. https://doi.org/10.3906/tar-2009-49 |
