Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process
Yıl: 2022 Cilt: 26 Sayı: 2 Sayfa Aralığı: 272 - 278 Metin Dili: İngilizce DOI: 10.29228/jrp.125 İndeks Tarihi: 07-06-2022
Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process
Öz: Nuclear receptors are a superfamily of transcription factors that regulate gene transcription and
intracellular function. They have a key role in various biological processes. Liver X receptors (LXR) mainly play
role in lipid metabolism, cholesterol synthesis, glucose homeostasis, and inflammation whereas retinoid X
receptor alpha (RXRα) plays important role in inflammation and immunity. In this study, Plantago holosteum, P.
major subsp. major, P. lagopus, P. scabra, and Scutellaria salviifolia were selected for further investigation due to
their anti-inflammatory activities. For this purpose, liver x receptor alpha (LXRα) and retinoid x receptor alpha
(RXRα) agonist activity of the extracts was determined through luciferase reporter gene assay on HEK293 cells
at a concentration of 100 μg/ml. P. holosteum, P. major subsp. major, P. lagopus, and P. scabra extracts showed weak
LXRα agonist activity with the fold values in a range of 0.95-1.39. P. major showed the highest agonist activity
among tested Plantago species. For LXRα agonist activity, S. salviifolia extract had a fold value of 2.37 which was
comparable to the positive control T09011317 at 10 nM concentration. Results for RXRα agonist activity found to
be similar to LXRα for all extracts. S. salviifolia showed the highest RXRα agonist activity with a fold value of
1.62. Results suggested that Scutellaria species, which are used as traditional medicine especially in eastern Asia
due to its anti-inflammatory effects, might show this effect due to LXRα activation.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- [1] Güner A, Aslan S, Ekim T, Vural M, Babaç MT. Türkiye Bitkileri Listesi (Damarli Bitkiler). Nezahat Gökyigit Botanik Bahçesi ve Flora Araştirmalari Dernegi Yayini. 2012.
- [2] Gonçalves S, Romano A. The medicinal potential of plants from the genus Plantago (Plantaginaceae). Ind Crops Prod. 2016;83:213-226. [CrossRef]
- [3] Genc Y, Harput US, Saracoglu I. Active compounds isolated from Plantago subulata L. via wound healing and antiinflammatory activity guided studies. J Ethnopharmacol. 2019;241. [CrossRef]
- [4] Genc Y. PhD Thesis. Pharmacognostical investigations on Plantago holosteumScop. Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey, 2017.
- [5] Taskova R, Evstatieva L, Handjieva N, Popov S. Iridoid patterns of genus Plantago L. and their systematic significance. Z Naturforsch C J Biosci. 2002;57(1-2):42-50. [CrossRef]
- [6] Shen J, Li P, Liu SS, Liu Q, Li Y, Sun Y, He C, Xiao P. Traditional uses, ten-years research progress on phytochemistry and pharmacology, and clinical studies of the genus Scutellaria. J Ethnopharmacol. 2021; 265: 113198. [CrossRef]
- [7] Wang S, Zheng Z, Weng Y, Yu Y, Zhang D, Fan W, Dai R, Hu Z. Angiogenesis and anti-angiogenesis activity of Chinese medicinal herbal extracts. Life Sci. 2004;74(20):2467-2478. [CrossRef]
- [8] Lin CC, Shieh DE, Yen MH. Hepatoprotective effect of the fractions of Ban-zhi-lian on experimental liver injuries in rats. J Ethnopharmacol. 1997;56(3):193-200. [CrossRef]
- [9] Zhang Z, Lian XY, Li S, Stringer JL. Characterization of chemical ingredients and anticonvulsant activity of American skullcap (Scutellaria lateriflora). Phytomedicine. 2009;16(5):485-493. [CrossRef]
- [10] Dogan Z, Harput S, Saracoglu I. Studies on antioxidant potential and active compounds of Scutellaria salviifolia Bentham. Planta Med. 2013;79(13):1213. [CrossRef]
- [11] Lam TL, Lam ML, Au TK, Ip DT, Ng TB, Fong WP, Wan DCC. A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs. Life Sci. 2000;67(23):2889-2896. [CrossRef]
- [12] Deng YX, Shi QZ, Chen B, Zhang XJ, Liu SZ, Qiu XM. Comparative pharmacokinetics of baicalin in normal and the type 2 diabetic rats after oral administration of the Radix scutellariae extract. Fitoterapia. 2012;83(8):1435-1442. [CrossRef]
- [13] Arituluk ZC, Ozkul Kocak C, Renda G, Ekizoglu M, Ezer N. Antimicrobial activity of three Scutellaria L. species from Turkey. J Res Pharm. 2019;23(3):552-558. [CrossRef]
- [14] Dogan Z.PhD Thesis. Pharmacognostical Investigations on some Scutellaria L. species as source of drug raw material. Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey, 2018. [15] Dogan Z, Ishiuchi K, Makino T, Saracoglu I. Alpha-pyrone glycosides from Scutellaria salviifolia Benth. Turk J Chem. 2019;43(3):972-981. [CrossRef]
- [16] Yilmaz G, Cicek M, Demirci B, Baser KHC. Essential oil compositions of subspecies of Scutellaria brevibracteata Stapf from Turkey. J Essent Oil Res. 2019;31(4):255-262. [CrossRef]
- [17] Bardakci H, Skaltsa H, Milosevic-Ifantis T, Lazari D, Hadjipavlou-Litina D, Yesilada E, et al. Antioxidant activities of several Scutellaria taxa and bioactive phytoconstituents from Scutellaria hastifolia L. Ind Crops Prod. 2015;77:196-203. [CrossRef]
- [18] Willy PJ, Umesono K, Ong ES, Evans RM, Heyman RA, Mangelsdorf DJ. Lxr, a nuclear receptor that defines a distinct retinoid responsepathway. Gene Dev. 1995;9(9):1033-1045. [CrossRef]
- [19] Hiebl V, Ladurner A, Latkolik S, Dirsch VM. Natural products as modulators of the nuclear receptors and metabolic sensors LXR, FXR and RXR. Biotechnol Adv. 2018;36(6):1657-1698. [CrossRef]
- [20] Fessler MB. The challenges and promise of targeting the liver X receptors for treatment of inflammatory disease. Pharmacol Therapeut. 2018;181:1-12. [CrossRef]
- [21] Wang W, Nakashima K, Hirai T, Inoue M. Anti-inflammatory effects of naturally occurring retinoid X receptor agonists isolated from Sophora tonkinensis Gagnep. via retinoid X receptor/liver X receptor heterodimers. J Nat Med-Tokyo. 2019;73(2):419-430. [CrossRef]
- [22] Saba E, Irfan M, Jeong D, Ameer K, Lee YY, Park CK, Hong S, Rhee MH. Mediation of antiinflammatory effects of Rg3-enriched red ginseng extract from Korean Red Ginseng via retinoid X receptor alpha-peroxisomeproliferating receptor gamma nuclear receptors. J Ginseng Res. 2019;43(3):442-451. [CrossRef]
- [23] Zelcer N, Tontonoz P. Liver X receptors as integrators of metabolic and inflammatory signaling. J Clin Invest. 2006;116(3):607-614. [CrossRef]
- [24] Joseph SB, Castrillo A, Laffitte BA, Mangelsdorf DJ, Tontonoz P. Reciprocal regulation of inflammation and lipid metabolism by liver X receptors. Nat Med. 2003;9(2):213-219. [CrossRef]
- [25] Baytop T, Türkiye'de bitkilerle tedavi, Nobel Tıp Kitabevi, Istanbul, Turkey 1999.
- [26] Genc Y, Sohretoglu D, Harput US, Ishiuchi K, Makino T, Saracoglu I. Chemical constituents of Plantago holosteum and evaluation of their chemotaxonomic significance. Chem Nat Compd+. 2020;56(3):566-568. [CrossRef]
- [27] Dereli FTG, Genc Y, Saracoglu I, Akkol EK. Enzyme inhibitory assessment of the isolated constituents from Plantago holosteum Scop. Z Naturforsch C. 2020;75(3-4):121-128. [CrossRef]
- [28] Genc Y, Dereli FTG, Saracoglu I, Akkol EK. The inhibitory effects of isolated constituents from Plantago major subsp. major L. on collagenase, elastase and hyaluronidase enzymes: Potential wound healer. Saudi Pharm J. 2020;28(1):101-106. [CrossRef]
- [29] Harput US, Genc Y, Saracoglu I. Cytotoxic and antioxidative activities of Plantago lagopus L. and characterization of its bioactive compounds. Food Chem Toxicol. 2012;50(5):1554-1559. [CrossRef]
- [30] Galvez M, Martin-Cordero C, Lopez-Lazaro M, Cortes F, Ayuso MJ. Cytotoxic effect of Plantago spp. on cancer cell lines. JEthnopharmacol. 2003;88(2-3):125-130. [CrossRef]
- [31] Lin HL, Zhou J, Lin KC, Wang HJ, Liang ZH, Ren XS, Huang L, Xia C. Efficacy of Scutellaria baicalensis for the treatment of hand, foot, and mouth disease associated with encephalitis in patients infected with EV71: A Multicenter, Retrospective Analysis. Biomed Res Int. 2016; Article ID 5697571. [CrossRef]
- [32] Arweiler NB, Pergola G, Kuenz J, Hellwig E, Sculean A, Auschill TM. Clinical and antibacterial effect of an anti-inflammatory toothpaste formulation with Scutellaria baicalensis extract on experimental gingivitis. Clin Oral Invest. 2011;15(6):909-913. [CrossRef]
- [33] Seok JK, Kwak JY, Choi GW, An SM, Kwak JH, Seo HH, Suh HJ, Boo YC. Scutellaria radix extract as a natural UV protectant for human skin. Phytother Res. 2016;30(3):374-9. [CrossRef]
- [34] Hong GE, Kim JA, Nagappan A, Yumnam S, Lee HJ, Kim EH, Lee WS, Shin SC, Park HS, Kim GS. Flavonoids identified from Korean Scutellaria baicalensis Georgi inhibit inflammatory signaling by suppressing activation of NF-kappa B and MAPK in RAW 264.7 Cells. Evid-Based Compl Alt. 2013;Article ID 912031. [CrossRef]
- [35] Panche AN, Diwan AD, Chandra SR. Flavonoids: an overview. J Nutr Sci. 2016;5:e47. [CrossRef]
- [36] Nakashima K, Murakami T, Tanabe H, Inoue M. Identification of a naturally occurring retinoid X receptor agonist from Brazilian green propolis. Biochim Biophys Acta. 2014;1840(10):3034-3041. [CrossRef]
- [37] Kutluay VM, Inoue M, Saracoglu I. Assessment of LXR alpha agonist activity and selective antiproliferative efficacy: a study on different parts of Digitalis species. Turk J Biochem. 2019;44(6):745-751. [CrossRef]
| APA | KUTLUAY V, GENC Y, DOGAN Z, Inoue M, SARACOGLU I (2022). Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process. , 272 - 278. 10.29228/jrp.125 |
| Chicago | KUTLUAY Vahap Murat,GENC Yasin,DOGAN Zeynep,Inoue Makoto,SARACOGLU Iclal Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process. (2022): 272 - 278. 10.29228/jrp.125 |
| MLA | KUTLUAY Vahap Murat,GENC Yasin,DOGAN Zeynep,Inoue Makoto,SARACOGLU Iclal Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process. , 2022, ss.272 - 278. 10.29228/jrp.125 |
| AMA | KUTLUAY V,GENC Y,DOGAN Z,Inoue M,SARACOGLU I Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process. . 2022; 272 - 278. 10.29228/jrp.125 |
| Vancouver | KUTLUAY V,GENC Y,DOGAN Z,Inoue M,SARACOGLU I Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process. . 2022; 272 - 278. 10.29228/jrp.125 |
| IEEE | KUTLUAY V,GENC Y,DOGAN Z,Inoue M,SARACOGLU I "Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process." , ss.272 - 278, 2022. 10.29228/jrp.125 |
| ISNAD | KUTLUAY, Vahap Murat vd. "Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process". (2022), 272-278. https://doi.org/10.29228/jrp.125 |
| APA | KUTLUAY V, GENC Y, DOGAN Z, Inoue M, SARACOGLU I (2022). Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process. Journal of research in pharmacy (online), 26(2), 272 - 278. 10.29228/jrp.125 |
| Chicago | KUTLUAY Vahap Murat,GENC Yasin,DOGAN Zeynep,Inoue Makoto,SARACOGLU Iclal Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process. Journal of research in pharmacy (online) 26, no.2 (2022): 272 - 278. 10.29228/jrp.125 |
| MLA | KUTLUAY Vahap Murat,GENC Yasin,DOGAN Zeynep,Inoue Makoto,SARACOGLU Iclal Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process. Journal of research in pharmacy (online), vol.26, no.2, 2022, ss.272 - 278. 10.29228/jrp.125 |
| AMA | KUTLUAY V,GENC Y,DOGAN Z,Inoue M,SARACOGLU I Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process. Journal of research in pharmacy (online). 2022; 26(2): 272 - 278. 10.29228/jrp.125 |
| Vancouver | KUTLUAY V,GENC Y,DOGAN Z,Inoue M,SARACOGLU I Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process. Journal of research in pharmacy (online). 2022; 26(2): 272 - 278. 10.29228/jrp.125 |
| IEEE | KUTLUAY V,GENC Y,DOGAN Z,Inoue M,SARACOGLU I "Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process." Journal of research in pharmacy (online), 26, ss.272 - 278, 2022. 10.29228/jrp.125 |
| ISNAD | KUTLUAY, Vahap Murat vd. "Nuclear receptor agonist activity studies on some Plantago species and Scutellaria salviifolia Benth.: A particular focus on liver x receptor alpha and retinoid x receptor alpha connected with the inflammation process". Journal of research in pharmacy (online) 26/2 (2022), 272-278. https://doi.org/10.29228/jrp.125 |
