TY  - JOUR
TI  - Chemical profile by LC-Q-TOF-MS of Nigella sativa seed
extracts and in vitro antimicrobial activity on bacteria
which are determined resistance gene and isolated from
nosocomial infection
AB  - Healthcare-associated infection (HCAI) is a global health challenge and methicillin-resistant
Staphylococcus aureus (MRSA) and extended-spectrum beta-lactamases (ESBL) producing gram-negative bacilli, which
were isolated from HCAIs, are the most common antimicrobial-resistant pathogens. The present research aimed to
determine the chemical composition and antimicrobial activity of ethyl acetate and methanol extracts of Nigella sativa
seeds. Antimicrobial activity of plant extracts was observed against 20 clinical yeast (which isolated from swab samples
of tongue dorsal surfaces in patients with stomatitis), 18 clinical bacteria isolates (which found from HCAIs), and also
eight bacteria, and three yeast standard strains by using a broth microdilution assay. The methanol extract had the
highest inhibitory activity against S. aureus standard strain at 0.4 mg/mL. The ethyl acetate extract showed the highest
antibacterial activity against Bacillus cereus standard strain at 0.4 mg/mL. The methanol and ethyl acetate extracts did
not show inhibitory activity against Gram-negative bacteria and yeast isolates. The LC-Q-TOF-MS profiles of the
extracts were found to contain saponin, alkaloid, and fatty acid. Mass spectra revealed the presence of 14 fatty acids,
four saponins, and three alkaloids for methanol extract and 13 fatty acids, three saponins, and two alkaloids for ethyl
acetate extract.
AU  - SERVI, HÜSEYIN
AU  - AYSAL, Ayhan Ibrahim
AU  - KISA, ÖZGÜL
AU  - Satana, Dilek
DO  - 10.29228/jrp.127
PY  - 2022
JO  - Journal of research in pharmacy (online)
VL  - 26
IS  - 2
SN  - 2630-6344
SP  - 287
EP  - 297
DB  - TRDizin
UR  - http://search/yayin/detay/514855
ER  -