TY  - JOUR
TI  - L-Theanine Reduces Epileptiform Activity In Brain Slices
AB  - Objective: L-theanine is one of the main amino acid of tea plant which can cross the blood brain barrier. In the central nervous system, L-theanine has certain effects on a number of neurotransmitter systems. In this study we tested whether acute application of L-theanine could induce any seizure like or inhibitory activity in the brain slice. 
Materials and Methods: Effects of L-theanine on epileptiform activity were investigated in two different brain regions. Horizontal hippocampal-entorhinal cortex slices were obtained from 30-35 days old C57BL/6 mice. Extracellular field potentials were recorded from medial entorhinal cortex (EC) and CA3 region of hippocampus. Epileptiform activity was induced by application of 4 Aminopyridine (4AP, 100 µM) in the brain slices and 50 µM L-theanine was applied. From the recordings, the duration and frequencies of ictal like events as well as frequency and amplitude of inter-ictal like activities were calculated.
Results: L-theanine alone did not initiate any synchronized activity. Although, bath application of L-theanine after 4AP induces epileptiform acitivities, did not significantly alter the duration and frequency of ictal discharges, L-theanine attenuated the interictal discharges. In EC the frequencies of interictal discharges were significantly decreased. In CA3, the amplitudes of interictal activities were significantly reduced. 
Conclusion: The present findings suggest that L-theanine do not induce any seizure like event but rather had a suppressive effect on ongoing epileptiform activity.
AU  - abidin, ismail
AU  - Basoglu, Harun
AU  - KESER, HATICE
AU  - Aydin-Abidin, Selcen
AU  - Öztürk, Hilal
DO  - 10.53394/akd.1057342
PY  - 2022
JO  - Akdeniz Tıp Dergisi
VL  - 8
IS  - 2
SN  - 1300-1779
SP  - 121
EP  - 127
DB  - TRDizin
UR  - http://search/yayin/detay/514872
ER  -