Yıl: 2021 Cilt: 45 Sayı: 3 Sayfa Aralığı: 275 - 286 Metin Dili: İngilizce DOI: 10.3906/biy-2011-16 İndeks Tarihi: 07-06-2022

Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region

Öz:
Polyhydroxyalkanoate (PHA) is stored as an important carbon and energy source in bacterial cells. For biomedical applications, gram-positive bacteria can be better sources of PHAs, since they lack outer membrane lipopolysaccharide. Although gram-positive Streptomyces coelicolor A3(2) has been indicated as a high potential PHA producer, phaC gene that encodes the key enzyme PHA synthase in the metabolic pathway is not determined in its genome. BLAST search results of the GenBank database argued that SCO7613 could specify a putative polyhydroxyalkanoate synthase (PhaC) responsible for PHA biosynthesis. Deduced amino acid sequence of SCO7613 showed the presence of conserved lipase box like sequence, 555GASAG559, in which serine residue was present as the active nucleophile. Present study describes deletion of putative S. coelicolor phaC gene via PCR dependent method. We showed that SCO7613 is not an essential gene in S. coelicolor and its deletion affected PHA accumulation negatively although it is not ceased. Transcomplementation abolished the mutant phenotype, demonstrating that the decrease in PHA resulted from the deletion of SCO7613.
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APA Demir Z, DORUK T, Yagci N, Tunca Gedik S (2021). Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region. , 275 - 286. 10.3906/biy-2011-16
Chicago Demir Zeynep,DORUK TUGRUL,Yagci Nevin,Tunca Gedik Sedef Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region. (2021): 275 - 286. 10.3906/biy-2011-16
MLA Demir Zeynep,DORUK TUGRUL,Yagci Nevin,Tunca Gedik Sedef Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region. , 2021, ss.275 - 286. 10.3906/biy-2011-16
AMA Demir Z,DORUK T,Yagci N,Tunca Gedik S Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region. . 2021; 275 - 286. 10.3906/biy-2011-16
Vancouver Demir Z,DORUK T,Yagci N,Tunca Gedik S Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region. . 2021; 275 - 286. 10.3906/biy-2011-16
IEEE Demir Z,DORUK T,Yagci N,Tunca Gedik S "Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region." , ss.275 - 286, 2021. 10.3906/biy-2011-16
ISNAD Demir, Zeynep vd. "Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region". (2021), 275-286. https://doi.org/10.3906/biy-2011-16
APA Demir Z, DORUK T, Yagci N, Tunca Gedik S (2021). Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region. Turkish Journal of Biology, 45(3), 275 - 286. 10.3906/biy-2011-16
Chicago Demir Zeynep,DORUK TUGRUL,Yagci Nevin,Tunca Gedik Sedef Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region. Turkish Journal of Biology 45, no.3 (2021): 275 - 286. 10.3906/biy-2011-16
MLA Demir Zeynep,DORUK TUGRUL,Yagci Nevin,Tunca Gedik Sedef Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region. Turkish Journal of Biology, vol.45, no.3, 2021, ss.275 - 286. 10.3906/biy-2011-16
AMA Demir Z,DORUK T,Yagci N,Tunca Gedik S Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region. Turkish Journal of Biology. 2021; 45(3): 275 - 286. 10.3906/biy-2011-16
Vancouver Demir Z,DORUK T,Yagci N,Tunca Gedik S Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region. Turkish Journal of Biology. 2021; 45(3): 275 - 286. 10.3906/biy-2011-16
IEEE Demir Z,DORUK T,Yagci N,Tunca Gedik S "Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region." Turkish Journal of Biology, 45, ss.275 - 286, 2021. 10.3906/biy-2011-16
ISNAD Demir, Zeynep vd. "Polyhydroxyalkanoate accumulation in Streptomyces coelicolor affected by SCO7613 gene region". Turkish Journal of Biology 45/3 (2021), 275-286. https://doi.org/10.3906/biy-2011-16