TY  - JOUR
TI  - Eugenol aggravates UVA-induced cytotoxic and genotoxic
response in HaCaT human keratinocytes
AB  - Eugenol is a phenolic compound isolated from clove essential oil. It is used in dentistry, fragrance,
cosmetic, and food industries. According to the fragrance ingredient safety assessment report, eugenol does not cause
phototoxic reactions and genotoxicity. However, its effect on UV-induced cytotoxicity and genotoxicity has not been
well examined. Here in this study, cytotoxic and genotoxic effects of eugenol are investigated on UVA-induced damage
using human keratinocyte cells (HaCaT). HaCaT cells were treated with increasing concentrations of eugenol (10-500
μM) for 1 hour and irradiated with 5-10-15 J/cm2 UVA. 24 hours later the neutral red uptake (NRU) assay was used to
evaluate cytotoxicity. For genotoxicity assay cells were exposed to 1-10 μM eugenol for one hour and non-cytotoxic
UVA irradiation doses (1, 2.5 J/cm2) were used. The alkaline comet assay was carried out immediately after the UVA
irradiation to measure the genotoxic potential of eugenol. The cytotoxicity assay results indicate that eugenol caused a
cytotoxic effect in a dose-dependent manner in HaCaT cells and increasing doses of UVA-irradiation enhanced the
cytotoxic effect of eugenol. The alkaline comet assay results showed that eugenol causes DNA single-strand breaks and
increasing doses of UVA-irradiation aggravates the genotoxic potential of eugenol. These data demonstrate that eugenol
has cytotoxic and genotoxic potential and eugenol aggravates UVA-induced cytotoxic and genotoxic response in HaCaT
human keratinocytes.
AU  - JANNUZZI, Ayse Tarbin
DO  - 10.29228/jrp.133
PY  - 2022
JO  - Journal of research in pharmacy (online)
VL  - 26
IS  - 2
SN  - 2630-6344
SP  - 354
EP  - 361
DB  - TRDizin
UR  - http://search/yayin/detay/514922
ER  -