Yıl: 2022 Cilt: 26 Sayı: 2 Sayfa Aralığı: 370 - 382 Metin Dili: İngilizce DOI: 10.29228/jrp.135 İndeks Tarihi: 07-06-2022

Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity

Öz:
Berberine (BER) is a benzylisoquinoline alkaloid found in many plants. It has high water solubilitybut its bioavailability is low in oral administration due to the first-pass effect in the liver and intestine. Phytosomesare are prepared by complexing herbal active ingredients and phospholipids (PLs). The aim of this study is to prepare BERphospholipidcomplexes (BPCs) using the reverse phase evaporation method. In the preparation of the formulations, the effects of BER:PL ratio (w/w), reaction temperature (°C) and reaction time (h) on the specifications of the complexes such as particle size (PS), zeta potential (ZP) and encapsulation efficiency (EE%) were investigated. The distribution of PS of obtained complexes was ranging between 339 -1259 nm and their ZP were within -4,27- -5,15 mV. The drug EE% was relatively high. Quality by Design (QbD) methods have been used in the formulation design and selection of the optimum formulation. BPCs anti-inflammatory, analgesic and antipyretic activities are evaluated by using in vivo methods.Carrageenan-, Prostaglandin E2 (PGE2)- and serotonin-induced hind paw edema, acetic acid-induced increase in capillary permeability and subcutaneous air-pouch models for the anti-inflammatory activity, inhibition of pbenzoquinone- induced abdominal constriction and hot plate test for the analgesic activity, Freund’s complete adjuvantinduced pyrexia model for the antipyretic activity were used in mice and rats. Results showed that BPCs showed potent anti-inflammatory, analgesic and antipyretic activities at the dose of 209 mg/kg.
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APA AK A, AKKOL E, Aksu B, Karatas A (2022). Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. , 370 - 382. 10.29228/jrp.135
Chicago AK Ayça GÜNGÖR,AKKOL Esra KÜPELİ,Aksu Buket,Karatas Aysegul Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. (2022): 370 - 382. 10.29228/jrp.135
MLA AK Ayça GÜNGÖR,AKKOL Esra KÜPELİ,Aksu Buket,Karatas Aysegul Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. , 2022, ss.370 - 382. 10.29228/jrp.135
AMA AK A,AKKOL E,Aksu B,Karatas A Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. . 2022; 370 - 382. 10.29228/jrp.135
Vancouver AK A,AKKOL E,Aksu B,Karatas A Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. . 2022; 370 - 382. 10.29228/jrp.135
IEEE AK A,AKKOL E,Aksu B,Karatas A "Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity." , ss.370 - 382, 2022. 10.29228/jrp.135
ISNAD AK, Ayça GÜNGÖR vd. "Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity". (2022), 370-382. https://doi.org/10.29228/jrp.135
APA AK A, AKKOL E, Aksu B, Karatas A (2022). Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. Journal of research in pharmacy (online), 26(2), 370 - 382. 10.29228/jrp.135
Chicago AK Ayça GÜNGÖR,AKKOL Esra KÜPELİ,Aksu Buket,Karatas Aysegul Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. Journal of research in pharmacy (online) 26, no.2 (2022): 370 - 382. 10.29228/jrp.135
MLA AK Ayça GÜNGÖR,AKKOL Esra KÜPELİ,Aksu Buket,Karatas Aysegul Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. Journal of research in pharmacy (online), vol.26, no.2, 2022, ss.370 - 382. 10.29228/jrp.135
AMA AK A,AKKOL E,Aksu B,Karatas A Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. Journal of research in pharmacy (online). 2022; 26(2): 370 - 382. 10.29228/jrp.135
Vancouver AK A,AKKOL E,Aksu B,Karatas A Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity. Journal of research in pharmacy (online). 2022; 26(2): 370 - 382. 10.29228/jrp.135
IEEE AK A,AKKOL E,Aksu B,Karatas A "Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity." Journal of research in pharmacy (online), 26, ss.370 - 382, 2022. 10.29228/jrp.135
ISNAD AK, Ayça GÜNGÖR vd. "Preparation and optimization of berberine phospholipid complexes using QbD approach and in vivo evaluation for anti-inflammatory, analgesic and antipyretic activity". Journal of research in pharmacy (online) 26/2 (2022), 370-382. https://doi.org/10.29228/jrp.135