TY  - JOUR
TI  - Investigation of the efficacy of paclitaxel on some miRNAs profiles in breast
cancer stem cells
AB  - Understanding of the functions of microRNAs in breast cancer and breast cancer stem cells have been a hope for the
development of new molecular targeted therapies. Here, it is aimed to investigate the differences in the expression levels of let-7a, miR10b, miR-21, miR-125b, miR-145, miR-155, miR-200c, miR-221, miR-222 and miR-335, which associated with gene and proteins in
MCF-7 (parental) and MCF-7s (Mammosphere/stem cell-enriched population/CD44+/CD24-cells) cells treated with paclitaxel. MCF7s were obtained from parental MCF-7 cells. Cytotoxic activity of paclitaxel was determined by ATP assay. Total RNA isolation and
cDNA conversion were performed from the samples. Changes in expression levels of miRNAs were examined by RT-qPCR. Identified
target genes and proteins of miRNAs were analyzed with RT-qPCR and western blot analysis, respectively. miR-125b was significantly
expressed (2.0946-fold; p = 0.021) in MCF-7s cells compared to control after treatment with paclitaxel. Downregulation of SMO, STAT3,
NANOG, OCT4, SOX2, ERBB2 and ERBB3 and upregulation of TP53 genes were significant after 48 h treatment in MCF-7s cells.
Protein expressions of SOX2, OCT4, SMAD4, SOX2 and OCT4 also decreased. Paclitaxel induces miR-125b expression in MCF-7s cells.
Upregulation of miR-125b may be used as a biomarker for the prediction of response to paclitaxel treatment in breast cancer.
AU  - Ari, Ferda
AU  - akgün, oğuzhan
AU  - Ertürk, Elif
AU  - Ulukaya, Engin
AU  - ZEYBEK, Umit
AU  - Kucukali, Cem Ismail
DO  - 10.3906/biy-2103-46
PY  - 2021
JO  - Turkish Journal of Biology
VL  - 45
IS  - 5
SN  - 1300-0152
SP  - 613
EP  - 623
DB  - TRDizin
UR  - http://search/yayin/detay/514970
ER  -